The Effects of Solvents and Extraction Methods on the Antioxidant Activity of P. niruri

Hadzri, Hafizah Mohd.; Yunus, Mohd Azizi Che; Zhari, Salman; Rithwan, Fahim

doi:10.11113/jt.v68.3030

Cited by 11 publications

(9 citation statements)

References 31 publications

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“…Their results showed that low concentrations of ethanol and acetonitrile are favorable for the extraction of phenolics with high antioxidant activity. In another study, using Pinus niruri, methanol was more efficient than other solvents such as ethanol, hexane, and ethyl acetate, showing an enhanced extraction rate of phenolic and flavonoid compounds with higher biological activities [88].…”

Section: Extraction Solventsmentioning

confidence: 97%

“…Several studies have been done demonstrating the importance of these solvents in the recovery of natural molecules and active extracts from different plants and by-products [65,[84][85][86], including the lignocellulosic by-products [87]. Researchers also studied the influence of these different solvents in obtaining antioxidant phenolic compounds from pine by-products, and depending on the solvent used, the extracted fraction (extract composition) is different [59,85,88]. For example, in a work by Venkatesan and collaborators [85], the impact of different extraction solvents (such as ethanol, methanol, isopropanol, acetonitrile, and acetone) was analyzed to obtain phenolic extracts with antioxidant activity from Pinus densiflora bark.…”

Section: Extraction Solventsmentioning

confidence: 99%

“…The amount of these active constituents varies depending on the pine specie and geographical location of growth [71]. Moreover, the solvent polarity and different methods of extraction used contribute to the different content in natural antioxidant and antioxidant activity of the extracts [88]. It is very important to ensure the chemical stability of polyphenols during the extraction processes, using mild extraction methods.…”

Section: Polyphenols As Extracted Biocompoundsmentioning

confidence: 99%

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Green and Sustainable Valorization of Bioactive Phenolic Compounds from Pinus By-Products

et al. 2020

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In Europe, pine forests are one of the most extended forests formations, making pine residues and by-products an important source of compounds with high industrial interest as well as for bioenergy production. Moreover, the valorization of lumber industry residues is desirable from a circular economy perspective. Different extraction methods and solvents have been used, resulting in extracts with different constituents and consequently with different bioactivities. Recently, emerging and green technologies as ultrasounds, microwaves, supercritical fluids, pressurized liquids, and electric fields have appeared as promising tools for bioactive compounds extraction in alignment with the Green Chemistry principles. Pine extracts have attracted the researchers’ attention because of the positive bioproperties, such as anti-inflammatory, antimicrobial, anti-neurodegenerative, antitumoral, cardioprotective, etc., and potential industrial applications as functional foods, food additives as preservatives, nutraceuticals, pharmaceuticals, and cosmetics. Phenolic compounds are responsible for many of these bioactivities. However, there is not much information in the literature about the individual phenolic compounds of extracts from the pine species. The present review is about the reutilization of residues and by-products from the pine species, using ecofriendly technologies to obtain added-value bioactive compounds for industrial applications.

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Section: Extraction Solventsmentioning

confidence: 97%

Section: Extraction Solventsmentioning

confidence: 99%

Section: Polyphenols As Extracted Biocompoundsmentioning

confidence: 99%

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Green and Sustainable Valorization of Bioactive Phenolic Compounds from Pinus By-Products

et al. 2020

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“…Moreover, ethyl acetate and hexane promotes the extraction of lipophilic compounds, such as flavonoids and carotenoids (Bae et al, 2012). Another study also showed the ability of ethyl acetate to extract more flavonoid compounds from medicinal plant compared to other organic solvents (Mohd Hadzri et al, 2014). Natural antioxidants have various functions and show synergistic interactions.…”

Section: Discussionmentioning

confidence: 99%

From Weed to Medicinal Plant: Antioxidant Capacities and Phytochemicals of Various Extracts of Mikania micrantha

Ishak¹

2018

IJAB

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Mikania micrantha is commonly consumed as traditional medicine in some countries, including Malaysia. Little is known about the antioxidant properties and phytochemicals of M. micrantha. This study was aimed to investigate the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacities of the leaves and stems of M. micrantha of hot water, cold water, 70% ethanol, ethyl acetate, and hexane extracts. Folin-Ciocalteu and aluminium chloride colorimetric assays were used to determine the TPC and TFC, respectively. The antioxidant capacities were determined using rapid, inexpensive and small-scale microplate of five different antioxidant assays. Gas chromatography-mass spectrometry (GC-MS) was used to chemically profile and characterize the phytochemicals. In comparison with different solvents, the ethyl acetate stems (EAS) and leaves (EAL) extracts of M. micrantha had the significantly greatest TPC (141 ± 0.51 mg gallic acid equivalent/g) and TFC (70.1 ± 0.92 catechin equivalent/g), respectively. Moreover, EAS extract had the significantly greatest antioxidant capacities using DPPH (EC50 = 324 ± 61.4 μg/mL), ABTS (0.53 ± 0.01 mmol trolox equivalent/g), FRAP (1.28 ± 0.05 mmol Fe 2+ /g), phosphomolybdenum antioxidative power (219 ± 7.03 mg ascorbic acid equivalent/g), and β-carotene bleaching (108 ± 2.23%) assays. GC-MS analysis of EAS showed the presence of sesquiterpenes (30.46%), phenol (16.38%), and alkane hydrocarbons (10.45%), which may contribute to its antioxidant capacities. These findings suggest the stems extract of M. micrantha using ethyl acetate as the potential source of natural antioxidant agents and its utilization to prevent oxidative damage-related diseases could be further explored.

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“…In this study, supercritical carbon dioxide (SC-CO 2 ) extraction was introduced to attain a better yield extract of O. stamineus leaves compared with other extraction methods. Moreover, few studies have proved that SC-CO 2 extraction gave better yield of extraction on plant matrices such as manjakani [12], dukung anak [13], jering [14] and rubber seed [15] compared to conventional extraction method.…”

Section: Introductionmentioning

confidence: 99%

ENHANCEMENT AND OPTIMIZATION OF SINENSETIN EXTRACT FROM Orthosiphon stamineus USING SUPERCRTITICAL CARBON DIOXIDE EXTRACTION

Aziz¹,

Yunus²,

Lee³

et al. 2018

MJAS

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Sinensetin is a rare valuable flavonoid compound that contains 5 methoxy groups attached on the flavone backbone. This compound is abundantly established in citrus peel and can also be found in medicinal herb, 'misai kucing'. Scientifically, 'misai kucing' is known as Orthosiphon stamineus; mostly used to treat kidney stones by old folks. Orthosiphon stamineus was listed in National Key Economic Area (NKEA) as contributor towards the Malaysian Economic Growth. Thus, this study was conducted with objectives to determine suitable modifier for supercritical carbon dioxide (SC-CO 2 ) extraction and to optimize the SC-CO 2 extraction condition (pressure, temperature and average particle size) on the concentration of sinensetin. Water, methanol and acetone were used on the determination of modifier for SC-CO 2 extraction by using water bath extraction at 40 °C in 8 hours. The results showed that acetone gave the highest percentage of sinensetin extract of 0.11%. Therefore, acetone was used as a modifier to enhance the extraction of sinensetin. The concentration of sinensetin was increased and the highest percentage of sinensetin obtained was 6.99%. Response surface methodology (RSM) through Box-Behnken design via Design Expert Software was applied to optimize the SC-CO 2 conditions. The first-order polynomial was used to express the percentage of sinensetin with R 2 value of 0.542. The optimum SC-CO 2 operating condition was 30 MPa, 40 °C and 308.24 µm with sinensetin percentage of 4.35%. Hence, SC-CO 2 with small amount of modifier was the best method to extract sinensetin as compared to water bath extraction method. AbstrakSinensetin merupakan sebatian flavonoid yang jarang ditemui dan sangat berharga terdiri daripada 5 kumpulan metoksi yang terikat pada struktur flavone. Sebatian ini banyak dijumpai dalam kulit limau dan juga boleh didapati dalam herba 'misai kucing'. Secara saintifik, 'misai kucing' dikenali sebagai Orthosiphon stamineus; di mana 'misai kucing' digunakan untuk merawat batu karang oleh orang-orang tua. Orthosiphon stamineus telah disenaraikan dalam Kawasan Ekonomi Utama Negara (NKEA) sebagai penyumbang kepada pertumbuhan ekonomi negara. Oleh itu, kajian ini dijalankan dengan objektif untuk menentukan pelarut tambahan yang sesuai untuk pengekstrakan bendalir supergenting karbon dioksida (SC-CO 2 ) dan mengoptimumkan ISSN -2506 Ahmad Hazim et al: ENHANCEMENT AND OPTIMIZATION OF SINENSETIN EXTRACT FROMOrthosiphon stamineus USING SUPERCRTITICAL CARBON DIOXIDE EXTRACTION 868 pemboleh ubah SC-CO 2 (tekanan, suhu dan purata saiz zarah) terhadap kepekatan sinensetin. Air, metanol dan aseton telah digunakan untuk menentukan pelarut tambahan untuk pengekstrakan SC-CO 2 dengan menggunakan pengekstrakan rendaman air pada suhu 40 °C dalam masa 8 jam. Keputusan menunjukkan bahawa aseton memberikan peratusan sinensetin tertinggi sebanyak 0.11%. Oleh itu, aseton digunakan sebagai pelarut tambahan untuk meningkatkan pengekstrakan sinensetin dalam SC-CO 2 . Kepekatan sinensetin meningkat dan peratusan tertinggi sinen...

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The Effects of Solvents and Extraction Methods on the Antioxidant Activity of P. niruri

Cited by 11 publications

References 31 publications

Green and Sustainable Valorization of Bioactive Phenolic Compounds from Pinus By-Products

Green and Sustainable Valorization of Bioactive Phenolic Compounds from Pinus By-Products

From Weed to Medicinal Plant: Antioxidant Capacities and Phytochemicals of Various Extracts of Mikania micrantha

ENHANCEMENT AND OPTIMIZATION OF SINENSETIN EXTRACT FROM Orthosiphon stamineus USING SUPERCRTITICAL CARBON DIOXIDE EXTRACTION

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