The effects of selenium and vitamin E on lung tissue in rats with sepsis

Atli, Mustafa; Erikoğlu, Mehmet; Kaynak, Adnan; Esen, Hacı Hasan; Kurban, Sevil

doi:10.25011/cim.v35i2.16288

Cited by 17 publications

(18 citation statements)

References 26 publications

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“…Moreover, a more recent study suggested that the cytoprotective effects of silymarin can be attributed to normalization of lipid metabolism and preservation of mitochondrial energy production in high fat feeding-induced murine model of obesity [58]. Nevertheless, several studies illustrated the protective effects of other antioxidants such as N-acetylcysteine, resveratrol, vitamin E and curcumin on different organs following induction of sepsis [14,49,59,60].…”

Section: Discussionmentioning

confidence: 99%

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Silymarin protects against sepsis-induced acute liver and kidney injury via anti-inflammatory and antioxidant mechanisms in the rat

Al-Kadi

Ahmed

El‐Tahawy

et al. 2020

Journal of advanced Biomedical and Pharmaceutical Sciences

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Sepsis is a leading cause of death among intensive care patients. During sepsis, exaggerated reaction to infection leads to massive production of reactive oxygen species and inflammatory mediators, which eventually leads to multiple organ damage. Silymarin is a well-known antioxidant and cytoprotective agent, which showed protective effects in different models of disease. Thus, we hypothesized that silymarin would be protective against sepsis-induced liver and kidney injury. Sepsis was induced in rats by the cecal ligation and puncture (CLP) method. Rats were divided into sham, CLP-non-treated and CLP treated with silymarin (100 mg/kg, i.p. 1 h following CLP). After 24 h, rats were euthanized for blood and tissue samples, which were used for assessment of MDA, NO, GSH, IL-6 and TNF-α levels and SOD activity, in addition to renal and hepatic function parameters. Survival study was conducted using another set of animals following the same previously mentioned procedure. Silymarin showed protective effects evidenced by enhanced overall survival following sepsis (80% in silymarin-treated vs. 20% in septic group), in addition to improvement of hepatic and renal function parameters and reduction of MDA, NO, IL-6 and TNF-α levels. Moreover, silymarin supported the endogenous antioxidant mechanisms via elevation of GSH levels and reinforcement of SOD activity. In conclusion, silymarin protects against sepsis-induced hepatic and renal injury, possibly via antioxidant and anti-inflammatory mechanisms.

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Section: Discussionmentioning

confidence: 99%

“…Thus, these pathways are important targets to improve survival and prevent organ damage in septic patients. Indeed, several studies established the protective effect of antioxidants against sepsis-induced organ injury [7,[12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Silymarin protects against sepsis-induced acute liver and kidney injury via anti-inflammatory and antioxidant mechanisms in the rat

Al-Kadi

Ahmed

El‐Tahawy

et al. 2020

Journal of advanced Biomedical and Pharmaceutical Sciences

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“…It is known that coenzyme Q10 exerts a powerful antioxidant property by directly reacting with free radicals in the lysosome, the Golgi apparatus and the plasma membrane, in addition to the mitochondria (15). Although the effi cacy of antioxidant support treatment for sepsis has not been suffi ciently determined yet, there are many studies in which positive results have been obtained with antioxidant treatment (16,17,18,19).…”

Section: Discussionmentioning

confidence: 99%

Effect of coenzyme Q10 on organ damage in sepsis

Abitağaoğlu¹,

Akıncı²,

Saricaoglu³

et al. 2015

BLL

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OBJECTIVE: Investigating the effects of coenzyme Q10 on organ damage and survival on mice in cecal ligation perforation (CLP) model in sepsis. BACKGROUND: Coenzyme Q10 is an antioxidant molecule playing an important role in mitochondria. Mitochondrial dysfunction is an important mechanism in sepsis pathophysiology. METHODS: Nintyfour Swiss Albino male mice were divided into 8 groups. CLP was performed in Group I. Coenzyme Q10, 100 mg/kg subcutaneously, was given 5 hours after CLP to Group II and 20 hours after CLP to Group III. Sham operation was performed in Group IV, 100 mg/kg coenzyme Q10 subcutaneously was given 5 hours after sham operation to Group V and 20 hours after sham operation to Group VI. No operation was performed in Group VII; coenzyme Q10, 100 mg/kg subcutaneously, was given to Group VIII. Antibiotics and fl uid replacement were applied for 3 days. The mice still living were sacrifi ced at 576th hour. The organ damages were scored under light microscopy. RESULTS: The survival of Group I and Group II was lower than that of the control groups, but the survival in the Group III was similar to control groups. It was established that spleen, kidney, heart damage and total organ damage were decreased when compared to CLP group. CONCLUSIONS: Coenzyme Q10 is effective in decreasing histological organ damage in sepsis (Tab. 3. Fig. 1, Ref. 30).

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“…However, some studies indicated that rat CRP is used for the marker of inflammation (2,12,18). In this study it was determined that CRP concentrations were raised after administrations in three group but this increase was not statistically significant.…”

Section: Discussionmentioning

confidence: 99%

“…CRP is a useful marker of inflammatory clinical situation (12). In the rat CRP has been studied as a marker of inflammation using experimental models (2,12,18). But some researchers investigated that CRP was less sensitive marker of inflammation in the rat than in the human (3,6).…”

Section: Introductionmentioning

confidence: 99%

Procalcitonine, Nitric oxide and C-Reactive Protein concentrations in rats with experimentally-induced infectious and noninfectious inflammation

KIRAL

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of this study is to determine the alterations in procalcitonine (PCT), nitric oxide (NO) and C-reactive protein (CRP) levels in infectious and noninfectious inflammation and to evaluate their potential in the differentiation of inflammation. Totally 70 female Wistar rats (Group 1: n=21; Group 2, n=21, Group 3: n=21; Day 0: n=7), weighing 250-300 grams, were used in this study. Before the applications, blood samples were collected in seven rats and the time is accepted as Day 0. Three groups were formed each containing 21 rats. Stapylococcus aureus were administered at a concentration of 10 6 CFU in the infection group (Group 1), subcutaneously. Turbentine oil at a dose of 0.5 mg/kg were injected subcutaneously to twenty-one rats in the noninfectious inflammation group (Group 2). In the control group, 21 rats were injected with saline subcutaneously. Blood samples were collected from randomly selected 7 rats in each group on the 1 st , 4 th and the 7 th days. PCT, NO, and CRP analyzes were performed with commercial test kits in the ELISA reader. According to the obtained results, we determined that serum PCT, NO and CRP levels increased after administrations. The rises of CRP and PCT concentrations were not statistically significant but it was higher in infectious group levels than the other two groups. Based on these results, it can be said that NO shows significant increases during infectious inflammation and can be used for diferential diagnostic purposes in clinics. Key words: CRP, inflammation, NO, PCT, rat. Deneysel Olarak İnfeksiyöz ve Noninfeksiyöz Yangı Oluşturulmuş Ratlarda Prokalsitonin, Nitrik Oksit ve C-Reaktif Protein KonsantrasyonlarıÖzet: Bu çalışmanın amacı, infeksiyöz ve noninfeksiyöz yangı durumunda prokalsitonin(PCT), Nitrik oksit (NO) ve C-reaktif protein (CRP) düzeylerini belirlemek ve yangının ayrımının yapılmasında kullanılabilirliğini değerlendirmektir. Bu çalışmada toplam olarak 250-300 gram ağırlığında 70 dişi Wistar rat (Grup 1: n=21, Grup 2: n=21, Grup 3: n=21 ve 0. Gün n=7) kullanıldı. Uygulamalardan önce 7 rattan kan örnekleri alınıp 0. gün olarak kabul edildi. Ayrıca her biri 21 rat içeren 3 grup oluşturuldu. İnfeksiyon grubunda (Grup 1) bulunan 21 rata Staphylococcus aureus 10 6 CFU derialtı olarak verildi. Noninfeksiyöz yangı grubunda bulunan 21 rata Turbentin yağı 0.5 mg/kg derialtı uygulandı. Kontrol grubundaki 21 rata derialtı fizyolojik tuzlu su verildi. Uygulamalardan sonra her gruptan rastgele seçilen yedişer hayvandan, 1., 4. ve 7. günlerde kan örnekleri toplandı. PCT, CRP ve NO düzeyleri ELISA cihazında ticari test kitleriyle çalışıldı. Elde edilen sonuçlara göre PCT, NO ve CRP düzeylerinin uygulamalardan sonra arttığını belirlendi. CRP ve PCT düzeylerindeki artış infeksiyöz grupta diğer iki gruba göre daha fazlaydı ancak istatistiki önem saptanamadı. Bu çalışmanın sonuçlarına dayanarak, NO'in infeksiyöz yangıda önemli düzeyde arttığı ve kliniklerde ayırıcı tanı amaçlı kullanılabileceği söylenebilir.

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The effects of selenium and vitamin E on lung tissue in rats with sepsis

Cited by 17 publications

References 26 publications

Silymarin protects against sepsis-induced acute liver and kidney injury via anti-inflammatory and antioxidant mechanisms in the rat

Silymarin protects against sepsis-induced acute liver and kidney injury via anti-inflammatory and antioxidant mechanisms in the rat

Effect of coenzyme Q10 on organ damage in sepsis

Procalcitonine, Nitric oxide and C-Reactive Protein concentrations in rats with experimentally-induced infectious and noninfectious inflammation

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