The effects of rhBMP-2 released from biodegradable polyurethane/microsphere composite scaffolds on new bone formation in rat femora

Li, Bing; Yoshii, Toshitaka; Hafeman, Andrea E.; Nyman, Jeffrey S.; Wenke, Joseph C.; Guelcher, Scott A.

doi:10.1016/j.biomaterials.2009.08.038

Cited by 156 publications

(146 citation statements)

References 47 publications

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“…With an improved understanding and application of growth factors to improve bone regeneration, dual-delivery implants, implants that deliver both a growth factor and antimicrobial, may present a means to simultaneously promote bone growth and prevent infection. Arguably, biocompatible and bioabsorbable carriers that can deliver growth factors to improve bone regeneration [6][7][8][9] and antibiotics to prevent infection 8,[10][11][12][13] from the same implant may be superior therapeutics in the context of severe open fractures.…”

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confidence: 99%

Effect of various concentrations of antibiotics on osteogenic cell viability and activity

Rathbone

Cross

Brown

et al. 2011

Journal Orthopaedic Research

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Infection is a common complication of open fractures. Systemic antibiotics often cause adverse events before eradication of infected bone occurs. The local delivery of antibiotics and the use of implants that deliver both growth factors and antimicrobials are ways to circumvent systemic toxicity while decreasing infection and to reach extremely high levels required to treat bacterial biofilms. When choosing an antibiotic for a local delivery system, one should consider the effect that the antibiotic has on cell viability and osteogenic activity. To address this concern, osteoblasts were treated with 21 different antibiotics over 8 concentrations from 0 to 5,000 mg/ml. Osteoblast deoxyribonucleic acid content and alkaline phosphatase activity (ALP) were measured to determine cell number and osteogenic activity, respectively. Antibiotics that caused the greatest decrement include rifampin, minocycline, doxycycline, nafcillin, penicillin, ciprofloxacin, colistin methanesulfonate, and gentamicin; their cell number and ALP were significantly less than control at drug concentrations 200 mg/ ml. Conversely, amikacin, tobramycin, and vancomycin were the least cytotoxic and did not appreciably affect cell number and ALP until very high concentrations were used. This comprehensive evaluation of numerous antibiotics' effects on osteoblast viability and activity will enable clinicians and researchers to choose the optimal antibiotic for treatment of infection and maintenance of healthy host bone. ß

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mentioning

confidence: 99%

Effect of various concentrations of antibiotics on osteogenic cell viability and activity

Rathbone

Cross

Brown

et al. 2011

Journal Orthopaedic Research

Self Cite

289

224

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“…Previous studies have demonstrated that the addition of millimolar concentrations of calcium ions could enhance the proliferation and phenotype of osteoblast cells by membrane-mediated ion transfer. 33,34 Calcium ions play an important role in the regulation of osteoblastic proliferation and differentiation by changing the expression levels of specific calcium ion channel isoforms in osteoblasts. 35 In this study, we also compare the in vivo effect of SMC/ PHBHHx and PHBHHx on osteogenesis by implanting the rhBMP-2 adsorption scaffolds into the radius defects of New Zealand rabbits.…”

Section: Discussionmentioning

confidence: 99%

Siliceous mesostructured cellular foams/ poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite biomaterials for bone regeneration

Yang¹,

Xu²,

Zhou³

et al. 2014

IJN

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Osteoinductive and biodegradable composite biomaterials for bone regeneration were prepared by combining poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) with siliceous mesostructured cellular foams (SMC), using the porogen leaching method. Surface hydrophilicity, morphology, and recombinant human bone morphogenetic protein 2 adsorption/release behavior of the SMC/PHBHHx scaffolds were analyzed. Results of scanning electron microscopy indicated that the SMC was uniformly dispersed in the PHBHHx scaffolds, and SMC modification scaffolds have an interconnected porous architecture with pore sizes ranging from 200 to 400 μm. The measurements of the water contact angles suggested that the incorporation of SMC into PHBHHx improves the hydrophilicity of the composite. In vitro studies with simulated body fluid show great improvements to bioactivity and biodegradability versus pure PHBHHx scaffolds. Cell adhesion and cell proliferation on the scaffolds was also evaluated, and the new tools provide a better environment for human mesenchymal stem cell attachment, spreading, proliferation, and osteogenic differentiation on PHBHHx scaffolds. Moreover, micro-computed tomography and histological evaluation confirmed that the SMC/PHBHHx scaffolds improved the efficiency of new bone regeneration with excellent biocompatibility and biodegradability and faster and more effective osteogenesis in vivo.

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“…This modality has the benefit of being biodegradable while providing a scaffold for re-vascularization and bone formation [128]. It follows that manipulation of the scaffold to enable provision of osteoinductive matter has also been investigated to enable enhanced conditions for bone healing and infection control [129].…”

Section: Infection In Conflict Wounded-future Directions In Research mentioning

confidence: 99%

Infection in conflict wounded

Eardley

Brown

Bonner

et al. 2011

Phil. Trans. R. Soc. B

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Although mechanisms of modern military wounding may be distinct from those of ancient conflicts, the infectious sequelae of ballistic trauma and the evolving microbial flora of war wounds remain a considerable burden on both the injured combatant and their deployed medical systems. Battlefield surgeons of ancient times favoured suppuration in war wounding and as such Galenic encouragement of pus formation would hinder progress in wound care for centuries. Napoleonic surgeons eventually abandoned this mantra, embracing radical surgical intervention, primarily by amputation, to prevent infection. Later, microscopy enabled identification of microorganisms and characterization of wound flora. Concurrent advances in sanitation and evacuation enabled improved outcomes and establishment of modern military medical systems. Advances in medical doctrine and technology afford those injured in current conflicts with increasing survivability through rapid evacuation, sophisticated resuscitation and timely surgical intervention. Infectious complications in those that do survive, however, are a major concern. Addressing antibiotic use, nosocomial transmission and infectious sequelae are a current clinical management and research priority and will remain so in an era characterized by a massive burden of combat extremity injury. This paper provides a review of infection in combat wounding from a historical setting through to the modern evidence base.

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The effects of rhBMP-2 released from biodegradable polyurethane/microsphere composite scaffolds on new bone formation in rat femora

Cited by 156 publications

References 47 publications

Effect of various concentrations of antibiotics on osteogenic cell viability and activity

Effect of various concentrations of antibiotics on osteogenic cell viability and activity

Siliceous mesostructured cellular foams/ poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite biomaterials for bone regeneration

Infection in conflict wounded

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