The effects of Pueraria mirifica extract, diadzein and genistein in testosterone-induced prostate hyperplasia in male Sprague Dawley rats

Mohamad, Jamaludin; Masrudin, Siti Saleha; Alias, Zazali; Muhamad, Nur Airina

doi:10.1007/s11033-019-04638-5

Cited by 8 publications

(18 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Astaxanthin, at low concentration, was able to inhibit in vitro 5α-reductase by 98% and to decrease growth of cultured prostate cancer cell lines (96). The accumulation of zinc in prostate tissue depends, more than on dietary intake, on the activity of specific transporters (ZnT4 and ZIP4) that can be modulated by natural products as diadzein and genistein (97,98). In patients with symptomatic BPH a combination of zinc, daidzein and Isolase (a mixture of enzymes that increases the bioavailability of plant polyphenols) improved at 6-month follow up IPSS score, peak urinary flow rate and quality of life (99).…”

Section: Carotenoids and Mineral Supplements (Vittorio Magri)mentioning

confidence: 99%

Nutraceutical treatment and prevention of benign prostatic hyperplasia and prostate cancer

Cicero

Allkanjari

Busetto

et al. 2019

Arch Ital Urol Androl

View full text Add to dashboard Cite

During the last years, pharmaceutical innovations in primary care are dramatically less frequent and will be even more rare in the next future. In this context, preclinical and clinical research oriented their interest toward natural compounds efficacy and safety, supporting the development of a new “nutraceutical” science. Medicinal plants, in the form of plant parts or extracts of them, are commonly used for the treatment of prostate diseases such as benign hypertrophy, prostatitis and chronic pelvic pain syndrome. The pharmacological properties searched for the treatment of prostatic diseases are anti-androgenic, anti-estrogenic, antiproliferative, antioxidant and anti-inflammatory. The most studied and used medicinal plants are Serenoa repens, Pygeum africanum and Urtica dioica. Other promising plants are Cucurbita pepo, Epilobium spp, Lycopersum esculentum, Secale cereale, Roystonea regia, Vaccinium macrocarpon. In parallel, epidemiological studies demonstrated that diet may play an important role on incidence and development of prostatic diseases. The Mediterranean diet is rich of elements with anti-oxidant properties that act as a protective factor for prostatic cancer. Similarly, low intake of animal protein, high intake of fruits and vegetable, lycopene and zinc are a protective factor for benign prostatic hyperplasia (BPH). Serenoa repens in the treatment of symptoms of BPH has been tested either alone or, more frequently, in combination with other medicinal plants, alpha-blockers and inhibitors of 5- alpha reductase (5-ARI). Recent meta-analyses found the effectiveness of Serenoa repens similar or inferior of that of finasteride and tamsulosin but clearly higher than that of placebo in the treatment of mild and moderate low urinary tract symptoms (LUTS), nocturia and discomfort. Clinical trials showed potential synergistic effect of Serenoa repens with other medicinal plants and drugs. In addition to Serenoa repens, there are many other medicinal plants for which clinical evidence is still controversial. Urtica dioica, Pygeum africanum and Curcubita pepo can be considered as an adjunct to the common therapies and their use is supported by studies showing improvement of symptoms and flowmetric indices. Lycopene and selenium are natural products with antioxidant and anti-inflammatory action. The combination of lycopene and selenium with Serenoa repens was able to reduce inflammation in histological prostate sections and to further improve symptom scores and urinary flow in patients with BPH on tamsulosin treatment. Similar effects could be obtained with the use of other carotenoids, such as astaxanthin, and/or zinc. Efficacy on symptoms of patients with BPH of some polyphenols such as quercitin, equol and curcumin have been demonstrated by clinical studies. Pollen extract is a mixture of natural components able to inhibit several cytokines and prostaglandin and leukotriene synthesis resulting in a potent anti-inflammatory effect. Pollen extracts significantly improve symptoms, pain, and quality of life in patients affected by chronic pelvic pain syndrome and chronic prostatitis. Beta-sitosterol is a sterol able to improve urinary symptoms and flow measures, but not to reduce the size of the prostate gland. Palmitoylethanolamide (PEA) is an endogenous fatty acid amide-signaling molecule with anti-inflammatory and neuroprotective effects that can have an interesting role in the management of chronic pelvic pain syndrome and chronic urological pain. Finally, several plant-based products have been subjected to preclinical, in vitro and in vivo, investigations for their potential pharmacological activity against prostate cancer. Some epidemiological studies or clinical trials evaluated the effects of beverages, extracts or food preparations on the risk of prostate cancer. Some plant species deserved more intense investigation, such as Camelia sinensis (green or black tea), Solanum lycopersicum (common tomato), Punica granatum (pomegranate), Glycine max (common soy) and Linum usitatissimum (linen).

show abstract

Section: Carotenoids and Mineral Supplements (Vittorio Magri)mentioning

confidence: 99%

Nutraceutical treatment and prevention of benign prostatic hyperplasia and prostate cancer

Cicero

Allkanjari

Busetto

et al. 2019

Arch Ital Urol Androl

View full text Add to dashboard Cite

show abstract

“…Clinical observations confirmed possible effects of isoflavones on BPH/LUTSs, as preparations from isoflavone-rich plants partly reduced symptoms and the risk for symptomatic BPH [ 37 ]. However, previous experimental studies addressing isoflavone impacts in the prostate focused on estrogenic effects, hormone-disrupting properties, epithelial prostate cells, animal models and prostate cancer [ 38 , 39 , 40 , 41 , 42 , 43 ]. Consequently, the effects of isoflavones on human prostate smooth muscle contraction and on the growth of stromal cells are mostly unknown, and directed comparisons to identify the most active isoflavones in the prostate are still pending.…”

Section: Discussionmentioning

confidence: 99%

“…Our current findings, together with previous findings from epithelial cells, may suggest that genistein could reduce prostate growth in BPH in vivo, provided adequate concentrations occur in the prostate. In fact, genistein inhibited prostate growth in rodent models of testosterone-induced BPH and high-fat-induced prostate growth [ 38 , 40 , 43 ], as well as ex vivo growth in prostate tissues from patients with BPH [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…Similar to genistein, isoflavone-rich plant preparations reduced experimentally induced and age-related prostate growth in in vivo studies on rodents, including extracts from red clover, black soybeans, Kudzu ( Pueraria montana ) and Pueraria mirifica [ 38 , 40 , 42 , 50 , 51 ], as did a soy-based, phytoestrogen-rich diet [ 39 ]. These and similar preparations contain several different isoflavones, with compositions and predominant compounds varying between plant species.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of α1-Adrenergic, Non-Adrenergic and Neurogenic Human Prostate Smooth Muscle Contraction and of Stromal Cell Growth by the Isoflavones Genistein and Daidzein

Huang

Liu

et al. 2022

Nutrients

View full text Add to dashboard Cite

Isoflavone-rich legumes, including soy, are used for food production, as dietary supplements and in traditional medicine. Soy consumption correlates negatively with benign prostatic hyperplasia (BPH) and voiding symptoms. However, isoflavone effects on the prostate are hardly known. Here, we examined the effects on human prostate smooth muscle contractions and stromal cell growth, which are driving factors of voiding symptoms in BPH. Smooth muscle contractions were induced in prostate tissues from radical prostatectomy. Growth-related functions were studied in cultured stromal cells (WPMY-1). Neurogenic, α1-adrenergic and non-adrenergic contractions were strongly inhibited with 50 µM and by around 50% with 10 µM genistein. Daidzein inhibited neurogenic contractions using 10 and 100 µM. Agonist-induced contractions were inhibited by 100 µM but not 10 µM daidzein. A combination of 6 µM genistein with 5 µM daidzein still inhibited neurogenic and agonist-induced contractions. Proliferation of WPMY-1 cells was inhibited by genistein (>50%) and daidzein (<50%). Genistein induced apoptosis and cell death (by seven-fold relative to controls), while daidzein induced cell death (6.4-fold) without apoptosis. Viability was reduced by genistein (maximum: 87%) and daidzein (62%). In conclusion, soy isoflavones exert sustained effects on prostate smooth muscle contractions and stromal cell growth, which may explain the inverse relationships between soy-rich nutrition, BPH and voiding symptoms.

show abstract

“…No enhancement of anti-proliferative effect was observed for combinations of zinc and isoflavones in our study nor, to our knowledge, described in literature. However, isoflavones influenced zinc levels and expression of zinc transporters in prostate tissue in vivo [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Essential Elements and Isoflavonoids in the Prevention of Prostate Cancer

et al. 2022

View full text Add to dashboard Cite

The intake of selected minerals, especially zinc, calcium and selenium, and high consumption of dietary isoflavones are recognised as factors influencing prostate cancer risk. Moreover, changes in levels of some essential elements are characteristic of the disease. Here, we examined the combined effects of main dietary isoflavonoids (genistein, daidzein and its metabolite, equol) and minerals implicated in prostate cancer, namely zinc, selenium, copper, iron and calcium, on LNCaP prostate cancer cells proliferation. Secondly, we evaluated the influence of the combinations on genotoxicity of model mutagens, 4-nitroquinoline oxide (4NQO) and 2-aminoanthracene (2AA), in the umu test. All combinations of isoflavonoids and minerals inhibited prostate cancer cells growth. However, only mixtures with iron ions had significantly stronger effect than the phytochemicals. Interestingly, we observed that only genistein attenuated genotoxicity of 4NQO. The addition of any tested mineral abolished this effect. All tested isoflavonoids had anti-genotoxic activity against 2AA, which was significantly enhanced in the presence of copper sulphate. Our results indicate that the tested minerals in physiological concentrations had minimal influence on the anti-proliferative activity of isoflavonoids. However, they significantly modulated the anti-genotoxic effects of isoflavonoids against both metabolically activated and direct mutagens. Thus, the minerals intake and nutritional status may modulate protective action of isoflavonoids.

show abstract

The effects of Pueraria mirifica extract, diadzein and genistein in testosterone-induced prostate hyperplasia in male Sprague Dawley rats

Cited by 8 publications

References 68 publications

Nutraceutical treatment and prevention of benign prostatic hyperplasia and prostate cancer

Nutraceutical treatment and prevention of benign prostatic hyperplasia and prostate cancer

Inhibition of α1-Adrenergic, Non-Adrenergic and Neurogenic Human Prostate Smooth Muscle Contraction and of Stromal Cell Growth by the Isoflavones Genistein and Daidzein

Essential Elements and Isoflavonoids in the Prevention of Prostate Cancer

Contact Info

Product

Resources

About