The effects of proteases on proteins and glycoproteins of Dictyostelium discoideum plasma membranes

Parish, Roger W.; Schmidlin, Sylvia; Müller, Ursula

doi:10.1016/0014-4827(77)90292-0

Cited by 40 publications

(16 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasma membrane isolation, SDS-gel electrophoresis,amido black protein staining, concanavalin A-peroxidase glycoprotein staining and autoradiography of gels were carried out as in [4,5,9,13,14].…”

Section: Dictyostelium Discoideummentioning

confidence: 99%

See 1 more Smart Citation

Detection of developmentally controlled plasma membrane antigens of dictyostelium discoideum cells in SDS‐polyacrylamide gels

Parish¹,

Schmidlin²,

Parish³

1978

FEBS Letters

Self Cite

View full text Add to dashboard Cite

Section: Dictyostelium Discoideummentioning

confidence: 99%

“…The method chosen resembles the concanavalin A-peroxidase technique we use for detecting glycoproteins in SDS-gels [9]. Antisera against plasma membranes was added to gels of plasma membrane proteins; antibodies binding to specific proteins were detected using anti-IgG coupled to peroxidase [IO].…”

Section: Introductionmentioning

confidence: 99%

Detection of developmentally controlled plasma membrane antigens of dictyostelium discoideum cells in SDS‐polyacrylamide gels

Parish¹,

Schmidlin²,

Parish³

1978

FEBS Letters

Self Cite

View full text Add to dashboard Cite

“…Cell differentiation is largely the result of regulated gene activity which may involve the interaction of DNA with regulatory proteins [ 1,2]. The nucleosome is the prime structural unit of chromatin [3].…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic isolation of nucleosomes from Dictyostelium nuclei and nucleoli

et al. 1980

Self Cite

View full text Add to dashboard Cite

“…The current applied was 17.5 mamp and electrophoresis was continued for 1 h after the tracking dye ran off the gel ( 15 min for 12 cm gels). Gels were fixed and stained using standard methods with Coomassie brilliant blue R-250 for protein visualization or with a coupled Con A-peroxidase method for glycoprotein (24).…”

Section: Methodsmentioning

confidence: 99%

Orientation and Integrity of Plasma Membrane Vesicles Obtained from Carrot Protoplasts

Randall

Ruesink²

1983

Plant Physiol.

View full text Add to dashboard Cite

Two fractions enriched in plasma membrane derived from suspensioncultured carrot (Daucus carota L.) cells were examined to determine if they differed from each other either in physical nature or in orientation. Parameters studied included the protein composition of purified membranes derived from trypsinized and nontrypsinized protoplasts as well as from trypsinized purified plasma membranes, the effect of inhibitors and membrane perturbants on ATPase activity, the binding of acetyl-14(Cconcanavalin A to purified membrane fractions, and the competitive removal of lacetyl-"Clconcanavalin A from purified membranes derived from lacetyl-4"Cconcanavalin A-labeled protoplasts. One fraction (at density of 1.102 grams per cubic centimeter on Renografin gradients) appears to be a mixed population of 'tightly' sealed vesicles with the majority being rightside-out vesicles of plasma membrane, and the other fraction (density 1.128 grams per cubic centimeter) apparently is a population of predominantly 'leaky' vesicles and/or nonvesicular fragments of plasma membrane, a large portion of which appear to be 'leaky' inside-out vesicles. In addition, it is shown that plasma membraneenriched fractions can be distinguished from cellular endomembranes on the basis of protein and glycoprotein composition.Membrane preparations of high purity and known physical orientation (sidedness) have been extremely valuable in the elucidation of membrane properties in various non-plant systems (10,14,31). Knowledge of the purity and orientation of plasma membrane vesicles is critical for proper interpretation of transport and other biochemical activities of the plasma membrane such as cell wall synthesis. Relatively pure plant plasma membrane preparations have been obtained from a number ofsources (2,12,13,21,25). In only one instance (2) was the protein composition of the plasma membrane fraction (as determined by electrophoretic analysis) clearly distinguishable from a mitochondria-enriched fraction, but even this plasma membraneenriched fraction did not appear to be significantly different from other fractions enriched in Golgi, ER, and possibly tonoplast. This was attributed to the plasma membrane being a significant contaminant in the other fractions. Although a preparation may be primarily plasma membrane as measured by PACP3 staining (29), the protein composition as determined by electrophoretic

show abstract

The effects of proteases on proteins and glycoproteins of Dictyostelium discoideum plasma membranes

Cited by 40 publications

References 23 publications

Detection of developmentally controlled plasma membrane antigens of dictyostelium discoideum cells in SDS‐polyacrylamide gels

Detection of developmentally controlled plasma membrane antigens of dictyostelium discoideum cells in SDS‐polyacrylamide gels

Electrophoretic isolation of nucleosomes from Dictyostelium nuclei and nucleoli

Orientation and Integrity of Plasma Membrane Vesicles Obtained from Carrot Protoplasts

Contact Info

Product

Resources

About