Detection of developmentally controlled plasma membrane antigens of <i>dictyostelium discoideum</i> cells in SDS‐polyacrylamide gels

Parish, Roger W.; Schmidlin, Sylvia; Parish, C R

doi:10.1016/0014-5793(78)81031-x

Cited by 39 publications

(13 citation statements)

References 14 publications

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“…When synthesis of some developmentally regulated proteins ceases (e.g., 82 000 and 100 000 M r in D. discoideum, 71 000 M r in P. pallidum) they are subsequently lost from the plasma membrane [4,5,9]. The means by which these glycoproteins are selectively removed is unknown, although shedding or endocytosis followed by degradation may be involved.…”

Section: Resultsmentioning

confidence: 99%

“…Cells were labelled with . Plasma membrane isolation, SDS-gel electrophoresis, gel staining and autoradiography were as in [4,5,21 ].…”

Section: Methodsmentioning

confidence: 99%

“…Changes in the surface properties of cells accompany the development of the cellular slime molds [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17]. These changes are potentially involved in cellular adhesion, chemotaxis, aggregation and the induction of developmentally-regulated enzymes [ 18,19 ].…”

Section: Introductionmentioning

confidence: 99%

“…These changes are potentially involved in cellular adhesion, chemotaxis, aggregation and the induction of developmentally-regulated enzymes [ 18,19 ]. We have examined the plasma membrane proteins synthesized during development of the two species Dictyostelium discoideum [4][5][6] and Polysphondylium pallidum [8]. A number of proteins were synthesized at specific morphological stages.…”

Section: Introductionmentioning

confidence: 99%

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Labelling of Dictyostelium and Polysphondylium plasma membranes with a photosensitive hydrophobic probe

et al. 1981

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Section: Resultsmentioning

confidence: 99%

“…Cells were labelled with . Plasma membrane isolation, SDS-gel electrophoresis, gel staining and autoradiography were as in [4,5,21 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Labelling of Dictyostelium and Polysphondylium plasma membranes with a photosensitive hydrophobic probe

et al. 1981

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“…The synthesis of contact sites A is developmentally controlled, occurring during aggregation and ceasing when aggregation is complete [4,5]. The sites are subsequently lost from the membrane [4,6]. Contact sites A are antigenic [ l- 3,6] and cell adhesion can be completely blocked by univalent antibody fragments (Fab) directed against them [2].…”

Section: Introductionmentioning

confidence: 99%

Identification of a developmentally regulated plasma membrane glycoprotein involved in adhesion of polysphondylium pallidum cells

1979

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The position of the microtubule‐organizing center relative to the nucleus is independent of the direction of cell migration in dictyostelium discoideum

Sameshima

Imai²,

Hashimoto

1988

Cell Motil. Cytoskeleton

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Dictyostelium amoebae can migrate in several different modes. We tested for correlations of the direction of cell locomotion with the relative positions of the nucleus and microtubule-organizing center (MTOC). Five cases were analyzed on electron micrographs with a microcomputer. Each mode of movement showed characteristic locations of the MTOC relative to the nucleus; however, they differed in the various cases. In randomly migrating interphase amoebae, the number of cells with the MTOC located behind the nucleus was twice as great as those with the MTOC located ahead of the nucleus. During chemotactic migration toward folic acid, cells with the MTOC behind the nucleus were more numerous, with a concomitant reduction of anterior MTOCs.When amoebae aggregated on agar plates, a posterior location of the MTOC was most strikingly favored, whereas in cells aggregating under submerged conditions, the MTOC was indifferently anterior or posterior to the nucleus. (It may be significant that EDTA-resistant cell-cell adhesion was fully expressed in the former cells, but weaker in the latter.) Finally, in the case of chemotactically migrating cells from dissociated pseudoplasmodia, which adhere by means of other molecules, the MTOC was consistently ahead of the nucleus. Thus the MTOC shows no necessary preferential position anterior or posterior to the nucleus; its position, rather, correlates with the type of migration and perhaps with the nature of cell-cell adhesion.

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Detection of developmentally controlled plasma membrane antigens of dictyostelium discoideum cells in SDS‐polyacrylamide gels

Cited by 39 publications

References 14 publications

Labelling of Dictyostelium and Polysphondylium plasma membranes with a photosensitive hydrophobic probe

Labelling of Dictyostelium and Polysphondylium plasma membranes with a photosensitive hydrophobic probe

Identification of a developmentally regulated plasma membrane glycoprotein involved in adhesion of polysphondylium pallidum cells

The position of the microtubule‐organizing center relative to the nucleus is independent of the direction of cell migration in dictyostelium discoideum

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