The Effects of Pomegranate Seed Extract and β-Sitosterol on Rat Uterine Contractions

Promprom, Wilawan; Kupittayanant, P.; Indrapichate, Korakod; Wray, Susan; Kupittayanant, Sajeera

doi:10.1177/1933719109352687

Cited by 38 publications

(31 citation statements)

References 29 publications

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“…This could be similar to the contraction induced by wild ginger (Costus speciosus) rhizome [47] and pomegranate (Punica granatum L., Punicaceae) seed [48] extracts which was also shown to solely depend on the extracellular Ca 2+ . In this study, FDA binding to the muscarinic, oxytocin and PGF2α receptors may trigger the extracellular Ca 2+ influx prior to contraction.…”

Section: Discussionsupporting

confidence: 54%

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Salleh

Ahmad

2013

BMC Complement Altern Med

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BackgroundFicus deltoidea, is a perennial herb that is used to assist labor, firm the uterus post-delivery and to prevent postpartum bleeding. In view of its claimed uterotonic action, the mechanisms underlying plant’s effect on uterine contraction were investigated.MethodsAdult female SD rats were injected with 2 mg/kg 17β-oestradiol (E2) to synchronize their oestrous cycle. A day after injection, uteri were removed for in-vitro contraction studies. The dose dependent effect of Ficus deltoidea aqeous extract (FDA) on the tension produced by the isolated rat’s uteri was determined. The effects of atropine (2×10-8 M), atosiban (0.5 IU), THG113.31 (10 μM), oxodipine (0.25 mM), EDTA (1 mM), 2-amino-ethoxy-diphenylborate (2-APB) (40 mM) and thapsigargin (1 mM) on the maximum force of contraction (Emax) achieved following 2 mg/ml FDA administration were also investigated.ResultsFDA induced in-vitro contraction of the isolated rat’s uteri in a dose-dependent manner. Administration of atropine, atosiban and THG113.31 reduced the Emax with atosiban having the greatest effect. The Emax was also reduced following oxodipine and EDTA administration. There was no significant change observed following 2-APB administration. Thapsigargin, however, augmented Emax.ConclusionsFDA-induced contraction of the isolated rat’s uteri is mediated via multiple uterotonin receptors (muscarinic, oxytocin and prostaglandin F2α) and was dependent on the extracellular Ca2+. Contraction, however, was not dependent on the Ca2+ release from the internal stores. This in-vitro study provides the first scientific evidence on the claimed effect of Ficus Deltoidea on uterine contraction.

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Section: Discussionsupporting

confidence: 54%

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Salleh

Ahmad

2013

BMC Complement Altern Med

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“…It is suggested that the extract is a potent phasic activity stimulator in rat uterus, which happens due to the non-estrogenic effects of beta-sitosterol on inhibiting sarco-endoplasmic reticulum Ca 2+ -ATPase (SERCA) and K channel, which resulted in contraction by calcium entry on L-type calcium channels and myosin light chain kinase (MLCK). [58] It is demonstrated that pomegranate fruit extract has an embryonic protective nature against adrianycin-induced oxidative stress (adrianycin is a chemotherapeutic drug used in cancer treatment). [59] Moreover, pomegranate juice consumption could increase epididymal sperm concentration, motility, spermatogenic cell density, diameter of seminiferous tubules and germinal cell layer thickness.…”

Section: Chemical Compositionmentioning

confidence: 99%

Potent health effects of pomegranate

2014

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Accumulating data clearly claimed that Punica granatum L. (pomegranate) has several health benefits. Pomegranates can help prevent or treat various disease risk factors including high blood pressure, high cholesterol, oxidative stress, hyperglycemia, and inflammatory activities. It is demonstrated that certain components of pomegranate such as polyphenols have potential antioxidant, anti-inflammatory, and anticarcinogenic effects. The antioxidant potential of pomegranate juice is more than that of red wine and green tea, which is induced through ellagitannins and hydrosable tannins. Pomegranate juice can reduce macrophage oxidative stress, free radicals, and lipid peroxidation. Moreover, pomegranate fruit extract prevents cell growth and induces apoptosis, which can lead to its anticarcinogenic effects. In addition, promoter inhibition of some inflammatory markers and their production are blocked via ellagitannins. In this article, we highlight different studies on the therapeutic effects of pomegranate and their suggested mechanisms of actions.

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“…Phytosterols have also attracted attention as inhibitors of sarcoplasmic reticulum calcium ATPase and potassium ion channels [4,5]. As part of a collaboration investigating the structural influences on uptake and processing of sterol esters[6], we required semipreparative amounts of β-sitosterol.…”

Section: Introductionmentioning

confidence: 99%

A concise synthesis of β-sitosterol and other phytosterols

Hang

Dussault

2010

Steroids

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A convenient synthesis of sidechain-modified phytosterols is achieved via a temporary masking of the stigmasterol 5,6-alkene as an epoxide. Following performance of the desired modification, the alkene is regenerated through a mild deoxygenation. The approach is applied to the syntheses of β-sitosterol and campesterol acetate, and suggests a facile route to the (Z)-isomers of Δ 22-23 phytosterols.

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The Effects of Pomegranate Seed Extract and β-Sitosterol on Rat Uterine Contractions

Cited by 38 publications

References 29 publications

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

In-Vitro effect of Ficus deltoidea on the contraction of isolated rat’s uteri is mediated via multiple receptors binding and is dependent on extracellular calcium

Potent health effects of pomegranate

A concise synthesis of β-sitosterol and other phytosterols

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