The effects of phosphate on arsenic uptake and toxicity alleviation in tobacco genotypes with differing arsenic tolerances

Zvobgo, Gerald; Hu, Hongliang; Shang, Shenghua; Shamsi, Imran Haider; Zhang, Guoping

doi:10.1002/etc.2776

Cited by 22 publications

(13 citation statements)

References 39 publications

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“…These observations relate to the findings of the present study in which the exposed tobacco genotypes showed greater MDA content, which indicated high lipid peroxidation. Lead toxicity induces oxidative stress by accumulation of ROS . The ROS are capable of attacking and oxidizing all types of biomolecules, such as nucleic acids, proteins, and lipids .…”

Section: Discussionmentioning

confidence: 99%

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Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

Maodzeka

Hussain

Wei

et al. 2016

Enviro Toxic and Chemistry

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In the present study, the effects of lead (Pb) uptake and toxicity were investigated in a hydroponic culture using 7 tobacco (Nicotiana tabacum L.) genotypes (Bina 1 [B1], Kutsaga Mammoth 10 [KM10], Nanjing 3 [N3], Kutsaga 35 [K35], Kutsaga E1 [KE1], Cocker 176 [C176], and Kutsaga RK6 [KRK6]) that differed in Pb tolerance. Lead was applied as a solution of Pb nitrate at concentrations of 0 μM, 10 μM, 250 μM, and 500 μM. After 4 wk of Pb treatment, tissue biomass and photosynthetic parameters were measured and elemental analysis was performed. The results showed decreases in growth and photosynthetic parameters with increases in Pb concentration compared with the control. The least reduction in the recorded physiological parameters was noted in K35, whereas the greatest reduction was observed in N3, which is an obvious indication of genotypic differences. Activities of peroxidase, catalase, and malondialdehyde increased significantly with increases in Pb concentration, with genotypes K35 and N3 showing the least and the greatest reduction, respectively. The results demonstrate the phototoxic nature of Pb on plants, and it can be concluded that in Pb-prone areas genotypes K35 and B1 can be used for cultivation because they can grow efficiently in the presence of high Pb concentrations while restricting Pb uptake in the aboveground parts, as seen by the higher Pb tolerance index. Environ Toxicol Chem 2017;36:175-181. © 2016 SETAC.

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Section: Discussionmentioning

confidence: 99%

“…Lead toxicity induces oxidative stress by accumulation of ROS [8,[44][45][46]. The ROS are capable of attacking and oxidizing all types of biomolecules, such as nucleic acids, proteins, and lipids [1].…”

Section: Discussionmentioning

confidence: 99%

Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

Maodzeka

Hussain

Wei

et al. 2016

Enviro Toxic and Chemistry

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“…The inhibition of key enzyme systems leads to the formation of ROS, which in turn causes lipid peroxidation [39]. Many previous studies have demonstrated that the tolerant plant varieties or genotypes have better ability of scavenging ROS by producing higher levels of antioxidants [26,28,34]. The high levels of SOD, CAT, and POD activities in gannuoxiang indicate efficient scavenging of superoxide radical and H 2 O 2 .…”

Section: Shoot Rootmentioning

confidence: 99%

Varietal differences in the growth of rice seedlings exposed to perchlorate and their antioxidative defense mechanisms

Chen

et al. 2015

Enviro Toxic and Chemistry

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A hydroponic experiment was conducted to investigate perchlorate (ClO4 (-) ) phytotoxicity in different rice varieties. Considerable variations were observed when 24 rice varieties were treated with ClO4 (-) . The shoot height, root length, and biomass of most varieties were significantly reduced by ClO4 (-) . The roots were more sensitive than the shoots. Hierarchical clustering analysis demonstrated primarily 4 groups: ClO4 (-) -sensitive, medium ClO4 (-) -sensitive, medium ClO4 (-) tolerant, and ClO4 (-) -tolerant. Gannuoxiang (a ClO4 (-) -tolerant variety) and IR65598-112-2 (a ClO4 (-) -sensitive variety) were chosen to explore their antioxidant response when exposed to 0.2 mmol/L, 2.0 mmol/L, and 4.0 mmol/L ClO4 (-) . The results showed that the activities of superoxide dismutase and catalase increased in the shoots and roots of gannuoxiang with increasing doses of ClO4 (-) , but both of them decreased at higher concentrations of ClO4 (-) in IR65598-112-2. The addition of ClO4 (-) led to a significant increase in peroxidase activities for both of the varieties, whereas the increase was more pronounced in gannuoxiang than in IR65598-112-2. No significant difference was found in malondialdehyde (MDA) contents in gannuoxiang, whereas the addition of ClO4 (-) increased the MDA level significantly in IR65598-112-2. The results indicated that gannuoxiang has higher activities of antioxidant enzymes than IR65598-112-2 to cope with oxidative damage caused by ClO4 (-) stress, which may be the main cause of its high tolerance.

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“…Nicotiana tabacum is one of the most socially and economically important crops worldwide. It is not a hyperaccumulator of semimetals/metals although it is able to take and accumulate them at consistent level (Zvobgo et al 2015 ). In addition, it is a good candidate for phytoextraction because of its high biomass, moderate soil chemical/physical requirement, fast growth rate and easy to harvesting (Sarret et al 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of AtPCS1 in tobacco increases arsenic and arsenic plus cadmium accumulation and detoxification

Zanella

Fattorini

Brunetti

et al. 2015

Planta

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Main conclusionThe heterologous expression ofAtPCS1in tobacco plants exposed to arsenic plus cadmium enhances phytochelatin levels, root As/Cd accumulation and pollutants detoxification, but does not prevent root cyto-histological damages.High phytochelatin (PC) levels may be involved in accumulation and detoxification of both cadmium (Cd) and arsenic (As) in numerous plants. Although polluted environments are frequently characterized by As and Cd coexistence, how increased PC levels affect the adaptation of the entire plant and the response of its cells/tissues to a combined contamination by As and Cd needs investigation. Consequently, we analyzed tobacco seedlings overexpressing Arabidopsis phytochelatin synthase1 gene (AtPCS1) exposed to As and/or Cd, to evaluate the levels of PCs and As/Cd, the cyto-histological modifications of the roots and the Cd/As leaf extrusion ability. When exposed to As and/or Cd the plants overexpressing AtPCS1 showed higher PC levels, As plus Cd root accumulation, and detoxification ability than the non-overexpressing plants, but a blocked Cd-extrusion from the leaf trichomes. In all genotypes, As, and Cd in particular, damaged lateral root apices, enhancing cell-vacuolization, causing thinning and stretching of endodermis initial cells. Alterations also occurred in the primary structure region of the lateral roots, i.e., cell wall lignification in the external cortex, cell hypertrophy in the inner cortex, crushing of endodermis and stele, and nuclear hypertrophy. Altogether, As and/or Cd caused damage to the lateral roots (and not to the primary one), with such damage not counteracted by AtPCS1 overexpression. The latter, however, positively affected accumulation and detoxification to both pollutants, highlighting that Cd/As accumulation and detoxification due to PCS1 activity do not reduce the cyto-histological damage.Electronic supplementary materialThe online version of this article (doi:10.1007/s00425-015-2428-8) contains supplementary material, which is available to authorized users.

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The effects of phosphate on arsenic uptake and toxicity alleviation in tobacco genotypes with differing arsenic tolerances

Cited by 22 publications

References 39 publications

Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

Varietal differences in the growth of rice seedlings exposed to perchlorate and their antioxidative defense mechanisms

Overexpression of AtPCS1 in tobacco increases arsenic and arsenic plus cadmium accumulation and detoxification

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