Elucidating the physiological and biochemical responses of different tobacco (<i>Nicotiana tabacum</i>) genotypes to lead toxicity

Maodzeka, Antony; Hussain, N.; Wei, Liquan; Zvobgo, Gerald; Mapodzeke, James Mutemachani; Adil, Muhammad Faheem; Jabeen, Sidra; Feng, Wang; Jiang, Lixi; Shamsi, Imran Haider

doi:10.1002/etc.3522

Cited by 15 publications

(6 citation statements)

References 40 publications

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“…In-situ localization of Pb ions in roots revealed enhancement in Pb content in response to increment in metal concentration. Similar elevation in Pb content was reported in Oryza sativa 73 , Nicotiana tabaccum 74 , Medicago sativa 75 and Triticum aestivum 76 . The bulk of Pb in soil is absorbed by roots of plants and bind to the carboxyl group of mucilage uronic acids 77 .…”

Section: Discussionsupporting

confidence: 81%

In-situ localization and biochemical analysis of bio-molecules reveals Pb-stress amelioration in Brassica juncea L. by co-application of 24-Epibrassinolide and Salicylic Acid

Kohli

Bali

Tejpal

et al. 2019

Sci Rep

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Lead (Pb) toxicity is a major environmental concern affirming the need of proper mitigation strategies. In the present work, potential of combined treatment of 24-Epibrassinolide (24-EBL) and Salicylic acid (SA) against Pb toxicity to Brassica juncea L. seedlings were evaluated. Seedlings pre-imbibed in EBL (0.1 mM) and SA (1 mM) individually and in combination, were sown in Pb supplemented petri-plates (0.25, 0.50 and 0.75 mM). Various microscopic observations and biochemical analysis were made on 10 days old seedlings of B . juncea . The toxic effects of Pb were evident with enhancement in in-situ accumulation of Pb, hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), nuclear damage, membrane damage, cell death and polyamine. Furthermore, free amino acid were lowered in response to Pb toxicity. The levels of osmoprotectants including total carbohydrate, reducing sugars, trehalose, proline and glycine betaine were elevated in response to Pb treatment. Soaking treatment with combination of 24-EBL and SA led to effective amelioration of toxic effects of Pb. Reduction in Pb accumulation, reactive oxygen content (ROS), cellular damage and GSH levels were noticed in response to treatment with 24-EBL and SA individual and combined levels. The contents of free amino acid, amino acid profiling as well as in-situ localization of polyamine (spermidine) was recorded to be enhanced by co-application of 24-EBLand SA. Binary treatment of 24-EBL and SA, further elevated the content of osmoprotectants. The study revealed that co-application of combined treatment of 24-EBL and SA led to dimination of toxic effects of Pb in B . juncea seedlings.

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Section: Discussionsupporting

confidence: 81%

In-situ localization and biochemical analysis of bio-molecules reveals Pb-stress amelioration in Brassica juncea L. by co-application of 24-Epibrassinolide and Salicylic Acid

Kohli

Bali

Tejpal

et al. 2019

Sci Rep

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“…Obviously the addition of Ni and Pb induced a decrease in biomass value with the greatest decrease being noted in the tobacco cultivars after addition of N 1 (NPK+10 mg kg -1 Ni) and P 1 (NPK+10 mg kg -1 Pb) doses. Pb accumulation in plant tissue caused plant stress which affected plant root and shoot growth thereby leading to a reduction in biomass (Maodzeka et al, 2017). With increasing Pb applications, it was observed that there were statistically significant decreases in root and green parts dry matter yields (Kınay and Erdem, 2019).…”

Section: Biomassmentioning

confidence: 99%

“…Tobacco (Nicotiana tabacum L.) is recognized as an effective accumulator of metals from the soil, and can accumulate relatively high concentrations in its organs, mainly leaves (Mench et al, 1989;Doroszewska and Bebec, 2004;Vera-Estrella et al, 2017;Rong et al, 2020). It is reported that tobacco is able to produce high biomass rates, easy to find, can be grown over large areas, and can be accepted as hyper-accumulator for some heavy (Vanlı, 2007;Vamerali et al, 2010;da Silva et al, 2016;Maodzeka et al 2017;Palusińska et al, 2020). The uptake of heavy metal concentrations in tobacco leaves differs widely, and it mainly depends on the tobacco cultivars/genotypes, soil types or conditions, soil organic matter content, pH of the soil, environment and some other factors (Adamu et al, 1989;Wagner et al, 1988 ;Tsadilas et al, 2005;Piano et al, 2008;Golia et al, 2009;Zaprjanova et al, 2010;Regassa and Chandravanshi, 2016;Zhao et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

The effects of increasing doses of nickel and lead applications on some oriental tobacco varieties

Tepecik¹,

Irget²

2021

Turk J Agric For

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Heavy metals are hazardous pollutants for humans, animals and plants, when their threshold concentration exceeds. Tobacco can accumulate higher concentrations of heavy metals, and the genotypic differences of tobacco in heavy metal uptake and their growth responses have not been clearly examined. In this study, the effects of nickel (Ni) and lead (Pb) on phytoremediation capabilities were tested in four local Oriental tobacco cultivars (Basma, Akhisar, Sarıbağlar and Dibek). In two pot experiments, nitrogen (N), phosphorus (P) and potassium (P) were applied in fıxed doses, while Pb and Ni were applied at 4 different doses (10, 50, 100 and 150 mg kg -1 ). Plants were harvested after 50 days of growing period, and separated into roots, stems, leaves. The biomass values were measured, and Pb and Ni concentrations were quantified in plant organs. Results revealed that Basma cultivar had the highest total biomass value with 16.63 and 15.92 g pot -1 for Ni and Pb contents, respectively. While, the lowest total biomass was recorded in Dibek cultivar with 7.09 and 5.71 g pot -1 for Ni and Pb respectively. The biomass, Ni and Pb uptake and accumulation capabilities remained in the following order of cultivars Basma>Akhisar>Sarıbağlar>Dibek. Depending on the application doses, Ni and Pb concentrations of different plant parts (roots, stems and leaves) of all varieties showed significant (p<0.01) increases compared to the control treatments. All of the plant parts of Basma variety had higher Ni and Pb concentrations than the Akhisar, Sarıbağlar and Dibek variety. Nickel concentrations in the roots, stemsand leaves of different cultivars were determined to be the least in the control application and the highest in the NPK+150 mg kg -1 Ni (Ni 4 ) application. Lead concentrations in the roots, stems and leaves of different cultivars were determined to be the least in the control application and the highest in the NPK+150 mg kg -1 Pb (Pb 4 ) 3 application. Basma variety with higher Enrichment Factor (EF) and Bioaccumulation Factor (BAF) values were also found to be more effective than the other varieties for phytoremediation of Ni and Pb.The objective of this study was to examine the Ni and Pb uptake, transport and accumulation properties of oriental tobacco with special emphasis on its different varieties.

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“…Tobacco has the potential to accumulate Cd and Pb from contaminated soil [7]. Excessive Cd taken up by tobacco inhibits the absorption of N, P, and K, reducing the biomass of shoot and root [8], while Pb mainly influences seed germination and photosynthesis [9]. In contrast, Cu and Zn, which are essential mineral elements for plant growth, are crucial for maintaining chloroplast structure, enzyme activity, tobacco aroma and growth [10].…”

Section: Introductionmentioning

confidence: 99%

Humic Acid Reduces the Available Cadmium, Copper, Lead, and Zinc in Soil and Their Uptake by Tobacco

et al. 2020

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Tobacco (Nicotiana tabacum L.) is a crop that is able to accumulate metals. In this study, humic acid was selected as a Cd, Cu, Pb, and Zn passivator, and added to calcareous field soil in amounts of 6.4, 10.3, and 14.8 kg·ha−1. Its impact on the soil fractions of the metals in the soil was extracted by the Community Bureau of Reference (BCR) sequential extraction method, and their accumulation of the metals in tobacco leaves was investigated. Application of 14.8 kg·ha−1 humic acid decreased the DTPA-extracted concentrations of Pb, Cd, Zn, and Cu by 39%, 37%, 29%, and 18%, respectively, as compared with untreated soil. The fractions of Cd, Pb, Cu, and Zn in soil were extracted by the BCR sequential extraction method, and the relationship between the difference metal fractions in the soil and the metal contents in the plant materials were analyzed. The exchangeable fractions of Cd, Pb, Cu, and Zn and the reducible fractions of Pb and Cu are the main bioavailable fractions. Additionally, the reducible fractions of Cd and Zn, the oxidizable fractions of Pb, Cu, and Zn, and all residual fractions of metals were nonbioavailable fractions in the soil. The soils were treated with humic acid (HA) to shift bioavailable metals to stable phases that were less bioavailable. The available Cd, Pb, Cu, and Zn were strongly retained in the soil after the application of humic acid, which decreased the uptake in tobacco in the upper, middle, and lower leaves.

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Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

Cited by 15 publications

References 40 publications

In-situ localization and biochemical analysis of bio-molecules reveals Pb-stress amelioration in Brassica juncea L. by co-application of 24-Epibrassinolide and Salicylic Acid

In-situ localization and biochemical analysis of bio-molecules reveals Pb-stress amelioration in Brassica juncea L. by co-application of 24-Epibrassinolide and Salicylic Acid

The effects of increasing doses of nickel and lead applications on some oriental tobacco varieties

Humic Acid Reduces the Available Cadmium, Copper, Lead, and Zinc in Soil and Their Uptake by Tobacco

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