The effects of overexpression of formaldehyde dehydrogenase gene from Brevibacillus brevis on the physiological characteristics of tobacco under formaldehyde stress

Nian, Hongjuan; Meng, Qingchao; Cheng, Qipeng; Zhang, W.; Chen, L. M.

doi:10.1134/s1021443713060083

Cited by 8 publications

(2 citation statements)

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“…62 Moreover, as formaldehyde is detoxified in the C 1 pathway to formate via formaldehyde dehydrogenase (FALDH), transgenic tobacco overexpressing FALDH conferred tolerance up to formaldehyde concentrations of 6 mM. 63 Relative to wild type, the transgenic tobacco lines under formaldehyde stress produced higher biomass, total chlorophyll, protein, and soluble sugar content, as well as reduced levels of oxidative stress detected by lower levels of lipid peroxidation and H 2 O 2 as compared with the wild-type tobacco. Thus, future studies on the role of methanol in temperature acclimation could take advantage of transgenic plants overexpressing FALDH and demonstrated to have enhanced tolerance to formaldehyde stress.…”

Section: Discussionmentioning

confidence: 99%

“…Under moderately high levels, Arabidopsis seedlings exposed to 2–4 mM formaldehyde were observed to reduce total chlorophyll content, P n , and the expression of numerous photosynthesis related genes . Moreover, as formaldehyde is detoxified in the C 1 pathway to formate via formaldehyde dehydrogenase (FALDH), transgenic tobacco overexpressing FALDH conferred tolerance up to formaldehyde concentrations of 6 mM . Relative to wild type, the transgenic tobacco lines under formaldehyde stress produced higher biomass, total chlorophyll, protein, and soluble sugar content, as well as reduced levels of oxidative stress detected by lower levels of lipid peroxidation and H 2 O 2 as compared with the wild-type tobacco.…”

Section: Discussionmentioning

confidence: 99%

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High Temperature Acclimation of Leaf Gas Exchange, Photochemistry, and Metabolomic Profiles in Populus trichocarpa

Dewhirst

Handakumbura

Clendinen

et al. 2021

ACS Earth Space Chem.

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High temperatures alter the thermal sensitivities of numerous physiological and biochemical processes that impact tree growth and productivity. Foliar and root applications of methanol have been implicated in plant acclimation to high temperature via the C 1 pathway. Here, we characterized temperature acclimation at 35°C of leaf gas exchange, chlorophyll fluorescence, and extractable metabolites of potted Populus trichocarpa saplings and examined potential influences of mM concentrations of methanol added during soil watering over a twomonth period. Relative to plants grown under the low growth temperature (LGT), high growth temperature (HGT) plants showed a suppression of leaf water use and carbon cycling including transpiration (E), net photosynthesis (P n ), an estimate of photorespiration (R p ), and dark respiration (R d ), attributed to reductions in stomatal conductance and direct negative effects on gas exchange and photosynthetic machinery. In contrast, HGT plants showed an upregulation of nonphotochemical quenching (NPQ t ), the optimum temperature for ETR, and leaf isoprene emissions at 40°C. A large number of metabolites (867) were induced under HGT, many implicated in flavonoid biosynthesis highlighting a potentially protective role for these compounds. Methanol application did not significantly alter leaf gas exchange but slightly reduced the suppression of R d and R p by the high growth temperature while slightly impairing ETR, Fv′/Fm′, and q p . However, we were unable to determine if soil methanol was sufficiently taken up by the plant to have a direct effect on foliar processes. A small number of extracted leaf tissue metabolites (55 out of 10 015) showed significantly altered abundances under LGT and methanol treatments relative to water controls, and this increased in compound number ( 222) at the HGT. The results demonstrate the large physiological and biochemical impacts of high growth temperature on poplar seedlings and highlight the enhancement of the optimum temperature of ETR as a rapid thermal acclimation mechanism. Although no large effect on leaf physiology was observed, the results are consistent with methanol both impairing photochemistry of the light reactions via formaldehyde toxicity and stimulating photosynthesis and dark respiration through formate oxidation to CO 2 .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%