Mammalian CYP2E1 gene triggered changes of relative ions fluxes, CaM content and genes expression profiles in Petunia hybrida cells to enhance resistance to formaldehyde

Wu, Pan; Wang, Man; Zhang, Ting; Tong, Xiaxiu; Tu, Xiang

doi:10.1007/s11240-018-1476-8

Cited by 4 publications

(1 citation statement)

References 60 publications

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“…Referring to relevant studies on adventitious bud regeneration and speculation related to hormone transport, we therefore selected these genes from transcription factors related to bud regeneration for quantitative verification. To validate the DEGs, a total of 20 genes was selected for quantitative real-time PCR (qRT-PCR) analysis, following previously described methods [47]. Primers for qRT-PCR were designed using primer 3.0 (http://bioinfo.ut.ee/primer3-0.4.0, accessed on 17 March 2021) (Table S1).…”

Section: The Validation Of Candidate Genes By Qpcrmentioning

confidence: 99%

Insights into the Root Sprouts of Toona fargesii in a Natural Forest: From the Morphology, Physiology, and Transcriptome Levels

Cheng,

Ma,

Guo

et al. 2024

Forests

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Toona fargesii (T. fargesii) is a deciduous tree of the Meliaceae family which is utilized for high-value timber. Interestingly, root sprouting is a typical reproductive pattern in T. fargesii. Nevertheless, the genetics underlying this phenomenon are still unclear. Here, three type of roots of T. fargesii were used for histological observation, plant endogenous hormone determination, non-structural carbohydrate (NSC) determination, and sequencing using the Illumina next-generation and PacBio SMRT platforms. Our results indicated that root sprouts originated from cork cambiums. Furthermore, indole-3-acetic acid (IAA), zeatin riboside (ZR), gibberellic acid 3 (GA3), and abscisic acid (ABA) content was significantly increased, while soluble sugar content was significantly decreased in the root sprouts. In addition, transcriptomic analysis suggested a total of 36.19 G of raw data from which 210 differentially expressed genes (DEGs) of were identified in RS vs. SR. Of these, the candidate DEGs were largely enriched in the citrate cycle, gluconeogenesis, starch and sucrose metabolism, and plant hormone signal transduction pathways. We therefore speculated that the accumulation of cytokinin and auxin might be induced by ATP-binding cassette-B 19 (ABCB19) and ABCG14, which were necessary for root sprouting. Additionally, transcription factors SQUAMOSA promoter binding protein-like 18 (SPL18) and NAM, ATAF1/2, and CUC2-14 (NAC14) were found in response to environmental water and epigenetic modification in RS. Overall, this study was to unravel the physiological and transcriptomic levels of the development of root sprouting in T. fargesii.

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Section: The Validation Of Candidate Genes By Qpcrmentioning

confidence: 99%

Insights into the Root Sprouts of Toona fargesii in a Natural Forest: From the Morphology, Physiology, and Transcriptome Levels

Cheng,

Ma,

Guo

et al. 2024

Forests

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Effect of formaldehyde exposure on phytochemical content and functional activity of Agaricus bisporus (Lge.) Sing.

Kutluer

2024

Environ Sci Pollut Res

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In this study, the effect of formaldehyde on phytochemical content and antioxidant activity of Agaricus bisporus was investigated. Synthetic compost based on wheat straw was prepared by fermentation and disinfection. After steam pasteurization, 5 g of A. bisporus mycelia were inoculated into 1 kg of compost. To determine the effects of formaldehyde, 2, 4, and 6% concentrations were added to the composts, while compost without formaldehyde was used for the control group. The harvesting period was set at 10 weeks. Total phenolic and flavonoid content, macro- and microelement profile, and phenolic content were analyzed in the harvested A. bisporus samples. Macro- and microelement content was determined by ICP-OES, and phenolic compound profile was determined by LC-MS/MS analysis. Formaldehyde levels in A. bisporus samples were determined by the acetylacetone spectrophotometry method. The antioxidant capacity of A. bisporus samples was determined by DPPH scavenging activity; antimutagenic effects of samples were determined by Allium test. Application of 2, 4, and 6% formaldehyde resulted in a 1.12-, 1.19-, and 2.07-fold reduction in total phenolic content, respectively. The total phenolic content was reduced between 34.4% and 71.8%. These changes were confirmed by LC-MS/MS analysis. Compounds such as protocatechuic acid, salicylic acid, ferulic acid, and 4-OH benzoic acid, which were detected in the control group, could not be detected in the samples treated with 6% formaldehyde, and it was found that the application of formaldehyde reduced the phenolic content. Similar changes were also observed in macro- and microelements, and significant changes in elemental contents were observed after formaldehyde application. While the presence of formaldehyde at a low level, which may be due to natural production, was detected in the control group, a residue of 11.41 ± 0.93 mg/kg was determined in the 6% FMD applied group. All these changes resulted in a decrease in the antioxidant activity of A. bisporus. The DPPH scavenging activity, which was determined in the range of 21.6–73.3% in the control samples, decreased to 12.3–56.7% in the samples treated with formaldehyde. These results indicate that the application of formaldehyde at different stages of A. bisporus cultivation leads to significant changes in the nutritional value and biological activity of A. bisporus.

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Digital RNA-seq transcriptome plus tissue anatomy analyses reveal the developmental mechanism of the calabash-shaped root in Tetrastigma hemsleyanum

Bao

et al. 2021

Tree Physiology

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Tetrastigma hemsleyanum is a liana plant with promising medicinal and ornamental values. Its calabash-shaped roots (CRs) are served as a traditional Chinese herb. However, it takes a long growth period to form CRs. In the present study, three types of architectural roots, including fine roots (FRs), bar-shaped roots (BRs) and CRs, were employed as materials, and the characteristics of histo-anatomy and digital RNA-seq transcriptome profiles were analyzed. Among the three types of roots, the vascular bundles in FRs were intact, while some of the vascular bundles degenerated in BRs, and only few traces of vascular bundles existed in CRs. Meanwhile, no obvious cell inclusions were found in the cytoplasm of FRs, while a few inclusions were found in BRs, and abundant inclusions were detected in CRs, which might be the main source of medicinal components in roots. The transcriptome profiles and qRT-PCR validation indicated that seven up-regulated genes encoding xyloglucan glycosyltransferase, ACC oxidase, CYP711A1, SHORT-ROOT transcript factor, galacturonosyltransferas, WAT1 and WRKY, and two down-regulated genes encoded LRR receptor-like serine/threonine-protein kinase and CYP83B1, were probably involved in the formation and development of CRs. Besides, GO terms of intrinsic component of membrane, integral component of membrane, cell periphery, membrane part, plasma membrane, membrane, intrinsic component of plasma membrane, cellular chemical homeostasis, and plasma membrane part were probably related to the formation of CRs. KEGG pathways related to the development of CRs probably included MAPK signaling pathway-plant, plant hormone signal transduction, and circadian rhythm-plant. Our finding suggested a probable mode for the formation of CRs.

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Mammalian CYP2E1 gene triggered changes of relative ions fluxes, CaM content and genes expression profiles in Petunia hybrida cells to enhance resistance to formaldehyde

Cited by 4 publications

References 60 publications

Insights into the Root Sprouts of Toona fargesii in a Natural Forest: From the Morphology, Physiology, and Transcriptome Levels

Insights into the Root Sprouts of Toona fargesii in a Natural Forest: From the Morphology, Physiology, and Transcriptome Levels

Effect of formaldehyde exposure on phytochemical content and functional activity of Agaricus bisporus (Lge.) Sing.

Digital RNA-seq transcriptome plus tissue anatomy analyses reveal the developmental mechanism of the calabash-shaped root in Tetrastigma hemsleyanum

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