The Effects of Organic Solvents on the Physicochemical Properties of Human Serum Albumin Nanoparticles

Mohammad‐Beigi, Hossein; Shojaosadati, Seyed Abbas; Morshedi, Dina; Mirzazadeh, Negar; Arpanaei, Ayyoob

doi:10.15171/ijb.1168

Cited by 30 publications

(20 citation statements)

References 20 publications

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“…The most famous method of BSA preparation is the well-established desolvation technique (Marty et al., 1978 ); dehydration of the aqueous solution of BSA is carried out by the addition of an organic solvent such as acetone or ethanol. In this study, ethanol was used as the desolvating agent for its ability to solubilize the nonpolar groups in BSA disrupting its secondary structure with lesser volume compared to other solvents (Mohammad-Beigi et al., 2016 ). Moreover, it’s less toxic when compared to other solvents.…”

Section: Resultsmentioning

confidence: 99%

Chitosan-coated bovine serum albumin nanoparticles for topical tetrandrine delivery in glaucoma: in vitro and in vivo assessment

et al. 2022

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Glaucoma is one of the leading causes of blindness. Therapies available suffer from several drawbacks including low bioavailability, repeated administration and poor patient compliance with adverse effects thereafter. In this study, bovine serum albumin nanoparticles (BSA-NPs) coated with chitosan(CS) were developed for the topical delivery of tetrandrine (TET) for glaucoma management. Optimized nanoparticles were prepared by desolvation. pH, BSA, CS and cross-linking agent concentrations effects on BSA-NPs colloidal properties were investigated. CS-BSA-NPs with particle size 237.9 nm and zeta potential 24 mV was selected for further evaluation. EE% exceeded 95% with sustained release profile. In vitro mucoadhesion was evaluated based on changes in viscosity and zeta potential upon incubation with mucin. Ex vivo transcorneal permeation was significantly enhanced for CS coated formulation. In vitro cell culture studies on corneal stromal fibroblasts revealed NPs biocompatibility with enhanced cellular uptake and improved antioxidant and anti-proliferative properties for the CS-coated formulation. Moreover, BSA-NPs were nonirritant as shown by HET-CAM test. Also, bioavailability in rabbit aqueous humor showed 2-fold increase for CS-TET-BSA-NPs compared to TET with a sustained reduction in intraocular pressure in a rabbit glaucoma model. Overall, results suggest CS-BSA-NPs as a promising platform for topical ocular TET delivery in the management of glaucoma.

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Section: Resultsmentioning

confidence: 99%

Chitosan-coated bovine serum albumin nanoparticles for topical tetrandrine delivery in glaucoma: in vitro and in vivo assessment

et al. 2022

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“…Moreover, a lower volume of isopropyl alcohol is required to completely desolvate bovine serum albumin in comparison with ethanol [ 51 ]. A more complex relationship between properties of non-solvent and its influence on the size of nanoparticles, not limited to the difference of dielectric constants, was demonstrated by Mohammad-Beigi et al [ 52 ]. Pei et al, assumed that differences in alcohols’ viscosity can influence the desolvation of gelatin [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

et al. 2021

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Gelatin nanoparticles found numerous applications in drug delivery, bioimaging, immunotherapy, and vaccine development as well as in biotechnology and food science. Synthesis of gelatin nanoparticles is usually made by a two-step desolvation method, which, despite providing stable and homogeneous nanoparticles, has many limitations, namely complex procedure, low yields, and poor reproducibility of the first desolvation step. Herein, we present a modified one-step desolvation method, which enables the quick, simple, and reproducible synthesis of gelatin nanoparticles. Using the proposed method one can prepare gelatin nanoparticles from any type of gelatin with any bloom number, even with the lowest ones, which remains unattainable for the traditional two-step technique. The method relies on quick one-time addition of poor solvent (preferably isopropyl alcohol) to gelatin solution in the absence of stirring. We applied the modified desolvation method to synthesize nanoparticles from porcine, bovine, and fish gelatin with bloom values from 62 to 225 on the hundreds-of-milligram scale. Synthesized nanoparticles had average diameters between 130 and 190 nm and narrow size distribution. Yields of synthesis were 62–82% and can be further increased. Gelatin nanoparticles have good colloidal stability and withstand autoclaving. Moreover, they were non-toxic to human immune cells.

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“…Ethanol was used as desolvating agent due to its dielectric constant, dipole moment and solubility parameter that make it an ideal solvent to enter into the hydrophobic region of HSA, disrupt the hydrophilic layer of the protein in water, and to induce the denaturation of HSA and consequently the formation of NPs [53]. Besides, ethanol is less toxic compared to other organic solvents proposed as desolvating agents, i.e., acetone, methanol and acetonitrile [54,55].…”

Section: Hsa Nps Characteristicsmentioning

confidence: 99%

Chitosan coated human serum albumin nanoparticles: A promising strategy for nose-to-brain drug delivery

Piazzini¹,

Landucci²,

D’Ambrosio³

et al. 2019

International Journal of Biological Macromolecules

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The Effects of Organic Solvents on the Physicochemical Properties of Human Serum Albumin Nanoparticles

Cited by 30 publications

References 20 publications

Chitosan-coated bovine serum albumin nanoparticles for topical tetrandrine delivery in glaucoma: in vitro and in vivo assessment

Chitosan-coated bovine serum albumin nanoparticles for topical tetrandrine delivery in glaucoma: in vitro and in vivo assessment

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

Chitosan coated human serum albumin nanoparticles: A promising strategy for nose-to-brain drug delivery

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