The Effects of Natural Clinoptilolite and Nano-Sized Clinoptilolite Supplementation on Lipid Profile, Food Intakes and Body Weight in Rats with Streptozotocin-Induced Diabetes

Abstract

Purpose: To determine the effect of natural clinoptilolite (CLN) and nano-sized clinoptilolite (NCLN) on lipid profile, food intakes (FI) and weight changes in streptozotocin (STZ) induced diabetic rats.Methods: In this experimental study, 36 rats were randomly divided into two groups: diabetic group which was injected STZ (60 mg/kg BW), and a non-diabetic group. Three days after diabetes induction, each of these groups was randomly divided into 3 subgroups of 6 animals ((1) control, (2) 1%/food CLN, (3) 1%/food NCLN). The animals were supplemented for 28 days, starting three days after STZ administration. At the end of the study, blood was drawn for biochemical assays. The weights and FIs of the rats were measured at the beginning and end of each week.Results: Our findings revealed that there was no significant change in lipid profile, 28 days after administration of STZ in diabetic rats. Low density lipoprotein (LDL) was increased slightly in diabetic rats treated with NCLN without any significant changes in other lipid profile parameters in the other groups. Weight was reduced significantly in diabetic rats. Administration of CLN and NCLN prevented further weight loss in diabetic rats. All groups treated with STZ had higher food intake during the study.Conclusion: Lack of beneficial changes in lipid profile may be attributed to short study duration, insufficient for appearance of lipid abnormalities. Given the partial improvement in weight status and lack of undesirable effects of clinoptilolite supplementation, further research is recommended in subjects with typ1 diabetes mellitus.

Potential of Nanomaterial Applications in Dietary Supplements and Foods for Special Medical Purposes

“…In STZ induced diabetic rats treated with nanosized clinoptilolite, blood glucose was found to decrease to near normal levels (12.4 vs. 27.5 mmol/L), but no significant impact on oxidative stress markers was estimated [ 202 ]. Nanosized clinoptilolite injected to STZ induced diabetic rats caused a partial improvement in their weight status and lack of undesirable effects, although beneficial changes in lipid profile were not detected, which could be connected with short study duration [ 203 ].…”

Corinthian Currants Supplementation Restores Serum Polar Phenolic Compounds, Reduces IL-1beta, and Exerts Beneficial Effects on Gut Microbiota in the Streptozotocin-Induced Type-1 Diabetic Rat

“…Diabetic animal models developed polyuria, polyphagia, and polydipsia accompanied by weight loss, symptoms indicative of T1D [ 34 ]. The lower (p < 0.001) body weight of diabetic animals highlights the catabolic state of poor glycemic control, widely reported in T1D disease and manifested as a result of STZ-induced diabetes [ 59 , 60 ]. Dietary intervention with Corinthian currants did not affect body weight.…”

Nutrient intake, digestibility, growth performance, and carcass of sheep fed urea‐based diet supplemented with natural clinoptilolite

“…Higher CP digestibility may actually reflect a greater extent of ruminal CP degradation, which indicates that zeolite controlled the availability of NH 3 ‐N concentration in the rumen (El‐Nile et al, 2021), and a lot of ammonia was available for more of microbial proteins synthesis. However, the dietary supplementation with zeolite increases intestinal hypertrophied functions of intestinal villi and epithelial cells which allows for extra surface area for nutrient absorption (Hossein‐Nia et al, 2018) and thus improves nutrient digestibility (Khambualai et al, 2009). Additionally, zeolite is composed of mobile minerals and interchangeable ions with a great selectivity for either ammonia (Çulfaz & Yağız, 2004) or some co‐minerals, which are necessary for proliferation of several cellulolytic microorganisms in the rumen (Galindo et al, 1990).…”