The effects of n-acetylcysteine on methotrexate-induced oxidative renal damage in rats

Çetinkaya, Ali; Kurutaş, Ergül Belge; Bülbüloğlu, Ertan; Kantarçeken, Bülent

doi:10.1093/ndt/gfl420

Cited by 66 publications

(98 citation statements)

References 4 publications

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“…Tissue CAT is an important oxidant detoxifier, playing a key role in the elimination of lipid peroxides and H 2 O 2. Moreover, CAT can reduce peroxides by donating hydrogen (7). In addition, our results showed increased levels of CAT activity in the group administered only Vit C. However, the MTX alone group showed statistically decreased CAT activity.…”

Section: Discussionsupporting

confidence: 44%

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

et al. 2017

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Like several other anticancer drugs, methotrexate (MTX) causes side effects, such as neuropathic pain, hepatotoxicity, and nephrotoxicity. Abnormal production of reactive oxygen species has been suspected in the pathophysiology of MTX-induced hepatorenal toxicity. Therefore, the aim of this study was to investigate the probable protective role of vitamin C (Vit C) on oxidative stress induced by MTX in the liver and kidney tissues of rats. A total of 32 rats were randomly and equally divided into four groups. The first group served as the control group. The second group received a single dose of 20 mg/kg of MTX intraperitoneally. To demonstrate our hypothesis, the third and the fourth groups received 250 mg/kg of Vit C for 3 days by oral gavage, with or without MTX treatment. At the end of the study, the liver and kidney tissues of the rats were collected and examined using histology. Both the tissues were assayed for malondialdehyde concentration and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. In hepatic and renal tissues, lipid peroxidation levels were increased, whereas SOD, CAT, and GSH-Px levels were decreased by MTX. All parameters, including CAT levels in hepatic tissue, were significantly restored after the administration of Vit C for 3 days. Similar to the biochemical findings, evidence of oxidative damage was examined in both types of tissues by histopathological examination. From the results of this study, we were able to observe that Vit C administration modulates the antioxidant redox system and reduces the renal and hepatic oxidative stress induced by MTX. Vit C can ameliorate the toxic effect of MTX in liver and kidney tissues of rat.

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Section: Discussionsupporting

confidence: 44%

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

et al. 2017

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“…It was reported that 20 mg/kg dose of Mtx, used in this study, highly exceeds interval dose and can be accepted as high dose Mtx in human (Oktem et al, 2006;Cetinkaya et al, 2007;Abdel-Raheem and Khedr, 2014). Mtx treatment at high doses may cause renal failure (Abelson et al, 1983).…”

Section: Discussionmentioning

confidence: 78%

Effects of Lycopene Alone or Combined with Melatonin on Methotrexate-Induced Nephrotoxicity in Rats

Oğuz

Koçarslan²,

Tabur³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Methotrexate (Mtx), used for its anticancer and immunsuppresive properties, is known to be a nephrotoxic agent. We aimed to investigate the effects of lycopene (Lyc) alone or combined with melatonin (Mel) on Mtxinduced nephrotoxicity since both of these agents have antioxidant and anti-inflammatory effects. Nephrotoxicity was induced by intraperitoneal administration of methotrexate at a dose of 20 mg/kg. Treatment both with Lyc alone and Lyc combined with Mel provided significant reduction in tumor necrosis factor-alpha, interleukin 1-beta and ceruloplasmin levels in Mtx administered rats. Hovewer, Lyc combined with Mel provided a significant reduction also in NO levels. Hstopathological examination showed that there was an obvious improvement in the degenerative changes compared to Mtx administrated group with the Lyc combined Mel group giving best protection. In conclusion Lyc alone and combined with Mel provided significant improvement against renal damage caused by Mtx, preseumably via antioxidant and anti-inflammatory activities.

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“…In the study of Cetinkaya et al [16] investigating the effectiveness of NAC in oxidative liver injury due to methotrexate, NAC treatment was found to increase GSH levels and decrease the MDA and myeloperoxidase (MPO) levels. In light of these data, it was suggested that NAC could be used as a therapeutic agent in the prevention of hepatotoxicity in patients under methotrexate treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Grup II ve Grup III'de çekal ligasyon perforasyon (ÇLP) yöntemiyle sepsis modeli oluşturuldu. Grup III'e oral yoldan 0., 8. ve 16. saatte 20 mg/kg/gün NAC verildi.…”

Section: Gereç Ve Yöntemunclassified

The effect of N-acetyl cysteine on serum glutathione, TNF-α and tissue malondialdehyde levels in the treatment of sepsis

Gül¹,

Ayan²,

Seydanoğlu³

et al. 2011

Ulus Travma Acil Cerrahi Derg

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The effects of n-acetylcysteine on methotrexate-induced oxidative renal damage in rats

Cited by 66 publications

References 4 publications

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

Effects of Lycopene Alone or Combined with Melatonin on Methotrexate-Induced Nephrotoxicity in Rats

The effect of N-acetyl cysteine on serum glutathione, TNF-α and tissue malondialdehyde levels in the treatment of sepsis

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