The effect of N-acetyl cysteine on serum glutathione, TNF-α and tissue malondialdehyde levels in the treatment of sepsis

Gül, Mehmet; Ayan, Murat; Seydanoğlu, Abdüsselam; Cander, Başar; Girisgin, Sadik; Erayman, İbrahim; Erdem, Sami

doi:10.5505/tjtes.2011.66743

Cited by 10 publications

(2 citation statements)

References 13 publications

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“…SAAs include cysteine, cystine, taurine, and methionine. Animal experiments have confirmed that N-acetylcysteine can reduce oxidative stress damage and the release of inflammatory mediators, which is beneficial to sepsis recovery [ 22 , 23 ]. Other experimental studies have shown that acetylcysteine can reduce organ injury [ 24 ] and improve survival [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Amino Acid Concentration Profiles in Patients with Sepsis

Xie

et al. 2015

PLoS ONE

100

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ObjectivesThe goal of this work was to explore the dynamic concentration profiles of 42 amino acids and the significance of these profiles in relation to sepsis, with the aim of providing guidance for clinical therapies.MethodsThirty-five critically ill patients with sepsis were included. These patients were further divided into sepsis (12 cases) and severe sepsis (23 cases) groups or survivor (20 cases) and non-survivor (15 cases) groups. Serum samples from the patients were collected on days 1, 3, 5, 7, 10, and 14 following intensive care unit (ICU) admission, and the serum concentrations of 42 amino acids were measured.ResultsThe metabolic spectrum of the amino acids changed dramatically in patients with sepsis. As the disease progressed further or with poor prognosis, the levels of the different amino acids gradually increased, decreased, or fluctuated over time. The concentrations of sulfur-containing amino acids (SAAs), especially taurine, decreased significantly as the severity of sepsis worsened or with poor prognosis of the patient. The serum concentrations of SAAs, especially taurine, exhibited weak negative correlations with the Sequential Organ Failure Assessment (SOFA) (r=-0.319) and Acute Physiology and Chronic Health Evaluation (APACHE) II (r=-0.325) scores. The areas under the receiver operating characteristic curves of cystine, taurine, and SAA levels and the SOFA and APACHE II scores, which denoted disease prognosis, were 0.623, 0.674, 0.678, 0.86, and 0.857, respectively.ConclusionsCritically ill patients with disorders of amino acid metabolism, especially of SAAs such as cystine and taurine, may provide an indicator of the need for the nutritional support of sepsis in the clinic.Trial RegistrationClinicalTrial.gov identifier NCT01818830.

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Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Amino Acid Concentration Profiles in Patients with Sepsis

Xie

et al. 2015

PLoS ONE

100

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“…Numerosas publicaciones usan ratas con punción cecal como modelo de sepsis, utilizando diferentes dosis de NAC y vías de administración, ya sea vía oral con sonda 18,19 , en el agua que bebían dos días antes de la cirugía 1 , o en forma subcutánea en repetidas dosis antes de la cirugía 20 . En el actual trabajo se decidió usar la dosis de 150 mg/kg vía intraperitoneal, usada en varías publicaciones 21,22 , dado que se la consideró una vía de administración segura y exacta.…”

Section: Discussionunclassified

N-acetilcisteína eleva los niveles de creatinina sin disminuir las especies reactivas de oxígeno en ratas sépticas

Chapela¹,

Burgos²,

Muryan³

et al. 2021

RFEM

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En la sepsis se activa la cascada in amatoria y se desencadenan múltiples procesos siopatológicos. Uno de esos procesos es la producción de especies reactivas de oxígeno (ROS). Se han descrito diferentes fármacos, como la N-acetilcisteína (NAC), para reducir los niveles de ROS, pero ningún trabajo ha avalado su uso en la sepsis. Este estudio investigó la relación entre ROS y daño renal, generalmente presente en la sepsis, y su posible tratamiento con NAC en ratas. Se utilizaron ratas Sprague Dawley machos. Los animales se dividieron en dos grupos, uno recibió 150 mg/kg de NAC y el otro no. Además, cada grupo se dividió en 4 subgrupos (control, laparotomía, sepsis y sepsis y NAC). Se midieron la creatinina sérica y las ROS. Los subgrupos sépticos tuvieron niveles más altos de ROS que los controles y NAC no disminuyó esos niveles. No hubo diferencias en los niveles de ROS entre los subgrupos (tratados y no tratados con NAC), pero los niveles de creatinina fueron más altos. Se observó una correlación débil entre ROS y los niveles de creatinina y la administración de NAC fue un factor independiente para aumentar los niveles de creatinina. Se observó una correlación entre los niveles de ROS y la creatinina. NAC no redujo los niveles de ROS, pero aumentó los niveles de creatinina. Concluimos que la administración de NAC no mejoró los niveles de ROS y disminuyó la función renal en ratas sépticas. Hubo una correlación débil entre ROS y los niveles de creatinina, lo que demuestra que otros factores pueden modi car la función renal, como la administración de NAC, que fue un factor independiente para la disminución de la función renal medida por la creatinina sérica.

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α-Linolenic acid attenuates doxorubicin-induced cardiotoxicity in rats through suppression of oxidative stress and apoptosis

Yu¹,

Cui²,

Zhang³

et al. 2013

ABBS

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Doxorubicin (DOX), a widely used anti-tumor drug, can give rise to severe cardiotoxicity by oxidative stress and cell apoptosis, which restricts its clinical application. α-Linolenic acid (ALA) has been shown to serve as a potent cardioprotective agent. The aim of this study was to explore the protective effects of ALA on DOX-induced cardiotoxicity and the underlying molecular mechanisms for this cardioprotection in rats. Rats were randomly divided into four groups and administrated with normal saline, ALA (500 µg/kg), DOX (2.5 mg/kg), or ALA (500 µg/kg) plus DOX (2.5 mg/kg) for 17 days. The results showed that DOX treatment significantly increased the heart weight/body weight, liver wet weight (WW)/dry weight (DW), lung WW/DW, serum levels of brain natriuretic peptide, creatine kinase-MB, lactate dehydrogenase, and cardiac troponin I, myocardial necrosis and myocardial malondialdehyde content, and induced the mRNA expression of Nrf2 in the nucleus, cleaved caspase-3, Bax, and superoxide dismutase (SOD). In addition, DOX led to a significant decrease in left ventricular end-diastolic volume, stroke volume, ejection fraction, SOD, glutathione-peroxidase, catalase, as well as the expression of Kelch-like ECH-associated protein 1 (Keap1) in the cytoplasm, phospho-AKT, phospho-ERK, and Bcl-2. Co-treatment with ALA significantly eliminated these changes induced by DOX except further reduction of Keap1 and elevation of Nrf2 and SOD mRNA. These results showed the cardioprotective effects of ALA on DOX-induced cardiotoxicity in rats. The mechanisms might be associated with the enhancement of antioxidant defense system through activating Keap1/Nrf2 pathway and anti-apoptosis through activating protein kinase B/extracellular signal regulated kinase pathway. Our results suggested a promising future of ALA-based preventions and therapies for myocardial damage after administration of DOX.

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The effect of N-acetyl cysteine on serum glutathione, TNF-α and tissue malondialdehyde levels in the treatment of sepsis

Cited by 10 publications

References 13 publications

Dynamic Changes in Amino Acid Concentration Profiles in Patients with Sepsis

Dynamic Changes in Amino Acid Concentration Profiles in Patients with Sepsis

N-acetilcisteína eleva los niveles de creatinina sin disminuir las especies reactivas de oxígeno en ratas sépticas

α-Linolenic acid attenuates doxorubicin-induced cardiotoxicity in rats through suppression of oxidative stress and apoptosis

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The effect of N-acetyl cysteine on serum glutathione, TNF-α and tissue malondialdehyde levels in the treatment of sepsis

Cited by 10 publications

References 13 publications

Dynamic Changes in Amino Acid Concentration Profiles in Patients with Sepsis

Dynamic Changes in Amino Acid Concentration Profiles in Patients with Sepsis

N-acetilcisteína eleva los niveles de creatinina sin disminuir las especies reactivas de oxígeno en ratas sépticas

&alpha;-Linolenic acid attenuates doxorubicin-induced cardiotoxicity in rats through suppression of oxidative stress and apoptosis

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α-Linolenic acid attenuates doxorubicin-induced cardiotoxicity in rats through suppression of oxidative stress and apoptosis