The effects of N-acetyl-L-cysteine supplementation on in vitro porcine oocyte maturation and subsequent fertilisation and embryonic development

Whitaker, Brian; Casey, Sarah J.; Taupier, Rachel

doi:10.1071/rd12002

Cited by 23 publications

(18 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in all applied concentrations (0.1; 0.5 and 1.0 mM), SAC increased the % of cleaving zygotes following parthenogenetic activation. A similar effect has also been described in the case of cysteine, which in the study by the authors Li et al (2014) increased early embryo cleavage of porcine oocytes following ICSI, and also in the case of NAC, which improved the formation of male pronuclei and subsequent embryonic development (Whitaker, Casey & Taupier, 2012). On the basis of our results it is possible to conclude that SAC positively influences early embryo cleavage, a significant indicator of the quality of activated oocytes.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Peer Review #1 of "The antioxidative properties of S-allyl cysteine not only influence somatic cells but also improve early embryo cleavage in pigs (v0.1)"

2016

View full text Add to dashboard Cite

In vitro cultivation systems for oocytes and embryos are characterised by increased levels of reactive oxygen species (ROS), which can be balanced by the addition of suitable antioxidants. S-allyl cysteine (SAC) is a sulfur compound naturally occurring in garlic (Allium sativum), which is responsible for its high antioxidant properties. In this study we demonstrated the capacity of SAC (0.1, 0.5 and 1.0 mM) to reduce levels of ROS in maturing oocytes significantly after 24 (reduced by 90.33%, 82.87% and 91.62% respectively) and 48 hour (reduced by 86.35%, 94.42% and 99.05% respectively) cultivation, without leading to a disturbance of the standard course of meiotic maturation. Oocytes matured in the presence of SAC furthermore maintained reduced levels of ROS even 22 hours after parthenogenic activation (reduced by 66.33%, 61.64% and 57.80% respectively). In these oocytes we also demonstrated a growth of early embryo cleavage rate (increased by 33.34%, 35.00% and 35.00% respectively). SAC may be a valuable supplement to cultivation media.

show abstract

Section: Discussionsupporting

confidence: 89%

“…Manuscript to be reviewed include the amino acid cysteine, which reduces levels of ROS in maturing bovine oocytes (Morado et al, 2003). A cysteine derivative, N-acetyl cysteine (NAC), positively influences the formation of pronuclei and the development of blastocytes in vitro in pigs (Whitaker, Casey & Taupier, 2012).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "The antioxidative properties of S-allyl cysteine not only influence somatic cells but also improve early embryo cleavage in pigs (v0.1)"

2016

View full text Add to dashboard Cite

show abstract

“…N-acetyl cysteine (NAC), as a typical antioxidant with a thiol group and a precursor of cysteine, after being taken by the cell is used for GSH synthesis (Samuni, Goldstein, Dean, & Berk, 2013). Supplementing maturation medium with NAC had beneficial effect on developmental competence of the porcine oocytes (Whitaker, Casey, & Taupier, 2012). During the earliest stages of embryo development, up to the two-cell stage, glycine apparently functions as an organic osmolyte and is highly accumulated in the cell (Baltz & Tartia, 2010;Steeves & Baltz, 2005), which in turn can protect embryos against osmotic stress when they are cultured in hyperosmotic conditions (Dawson, Collins, & Baltz, 1998;Richards, Wang, Liu, & Baltz, 2010;Steeves et al, 2003).…”

mentioning

confidence: 99%

Antioxidants and glycine can improve the developmental competence of vitrified/warmed ovine immature oocytes

Ahmadi

Shirazi

Shams‐Esfandabadi

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

Contents Despite the numerous potential applications of oocyte cryopreservation, the poor success rate has limited its practical applications. In livestock, particularly in ovine, the oocytes have low developmental competence following vitrification/warming process. Considering the occurrence of osmotic and oxidative stresses during the vitrification/warming process, the application of antioxidants and osmolytes may improve the developmental competence of vitrified/warmed oocytes. In the present study, we aimed to evaluate the effects of the addition of ascorbic acid (AA) and N‐acetyl cysteine (NAC) as antioxidants and glycine as an organic osmolyte either to the vitrification/warming solutions (VWS) or to the IVM medium on the developmental competence of vitrified/warmed ovine germinal vesicle stage oocytes. The survival rate in the vitrified groups was significantly lower than fresh ones. In vitrified/warmed oocytes, there was no significant difference in survival rate between supplemented and non‐supplemented groups. The addition of AA and/or NAC to the VWS or IVM medium and adding glycine to the IVM medium reduced the proportion of apoptotic oocytes and fragmented embryos, which was reflected as an increase in the proportions of metaphase II stage oocytes and blastocyst production. The best result was achieved by supplementing the IVM medium with NAC. In our study condition, antioxidants and glycine could improve the developmental competence of vitrified/warmed ovine immature oocytes, especially when added during IVM.

show abstract

“…These researchers concluded that "GSH synthesized by intact cumulus cells during maturation culture improved oocyte maturation and played an important role in fertilization and embryonic development" (Maedomari et al, 2007). Many additional studies on supplementing porcine, bovine, macaque, and goat IVM medium with GSH or a GSH precursor exhibited positive effects on in vitro production of embryos (Zhou et al, 2008;Choe et al, 2010;Curnow et al, 2010;Lott et al, 2011;Nabenishi et al, 2011;Whitaker at al., 2012;Merton et al, 2013). However, one study in goats found that adding 1 mM GSH caused an increase in intracellular GSH in prepubertal goat oocytes but did not improve fertilization rates (Mayor et al, 2001).…”

Section: Previous Methods For Improving In Vitro Maturationmentioning

confidence: 99%

285 REGULATION OF OOCYTE MEIOTIC RESUMPTION USING cAMP MODULATORS IN BOVINE IN VITRO MATURATION

Farmer

Sarmiento-Guzmán

Bailey

et al. 2015

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

In vitro maturation (IVM) is a reproductive technique critical to in vitro embryo production (IVP). Currently, IVP has low efficiency due to an inadequate IVM system where premature meiotic resumption results in low oocyte viability. Meiotic arrest is regulated primarily by 3′,5′-cyclic adenosine monophosphate (cAMP). Some successful methods of improving IVM have utilised cAMP modulators to maintain high intraoocyte cAMP, delaying the onset of nuclear maturation and allowing cytoplasmic maturation to occur. The current experiment is a follow-up to previous work in which an extended 2-step maturation system was examined. In the previous experiments, we found that meiotic resumption was significantly delayed, but the overall maturation rates of extended IVM were about half those of standard IVM, suggesting that the effects of the modulators on the oocytes were not completely reversible. The current experiment compares cAMP concentrations of oocytes in this extended IVM to standard IVM to determine whether high cAMP is the cause of the low maturation rate. Bovine oocytes (n = 686) were obtained from mixed-breed cattle by transvaginal ultrasound-guided aspiration. Oocytes from each cow were divided into 2 groups: standard IVM and extended IVM. Standard IVM consists of a 23-h maturation composed of TCM-199 supplemented with 10% fetal bovine serum, sodium pyruvate, pen/strep, glutamine, and FSH, and cultured at 39°C in 5% CO2. Extended IVM consists of 2 steps: a pre-IVM phase composed of HEPES-TALP supplemented with 100 µM forskolin (FSK) and 500 µM 3-isobutyl-1-methylxanthine (IBMX) for 2 h at 39°C, followed by an extended IVM phase composed of standard IVM media supplemented with 20 µM cilostamide for 31 h (39°C, 5% CO2). Additionally, oocytes in the extended IVM treatment group where held in HEPES-TALP media with FSK and IBMX during the 2-h oocyte collection period in order to prevent any decrease in cAMP before the oocytes could be placed in the extended IVM media. Oocytes in standard IVM were sampled at 0, 8, and 23 h of maturation, while oocytes in extended IVM were sampled at 0, 8, 18, and 33 h of maturation. Cumulus cells were removed from all oocytes by vortexing in hyaluronidase solution. Oocytes were stored in groups of 10 at –80°C. A cAMP enzyme immunoassay (GE Healthcare) was performed to determine cAMP concentrations throughout standard and extended IVM. Assay results were analysed using an ANOVA followed by a Tukey's pairwise test (Sigma Stat 3.5) to detect significant differences (P < 0.05). Results indicated significantly higher cAMP levels in extended IVM oocytes during the first 3 h after collection using FSK and IBMX in the holding media (0.505 v. 1.006 fmol/oocyte, P = 0.035) but cAMP levels were not maintained in the cilostamide-only extended IVM medium. This suggests that high cAMP levels were not the cause of low maturation rates in extended IVM, since cAMP concentrations did decrease after 3 h. Possible negative effect of cilostamide on these oocytes may be suggested and need to be analysed.

show abstract

The effects of N-acetyl-L-cysteine supplementation on in vitro porcine oocyte maturation and subsequent fertilisation and embryonic development

Cited by 23 publications

References 33 publications

Peer Review #1 of "The antioxidative properties of S-allyl cysteine not only influence somatic cells but also improve early embryo cleavage in pigs (v0.1)"

Peer Review #1 of "The antioxidative properties of S-allyl cysteine not only influence somatic cells but also improve early embryo cleavage in pigs (v0.1)"

Antioxidants and glycine can improve the developmental competence of vitrified/warmed ovine immature oocytes

285 REGULATION OF OOCYTE MEIOTIC RESUMPTION USING cAMP MODULATORS IN BOVINE IN VITRO MATURATION

Contact Info

Product

Resources

About