The Effects of MMP Inhibitors on Human Salivary MMP Activity and Caries Progression in Rats

Sulkala, Merja; Wahlgren, Jaana; Larmas, Markku; Sorsa, Timo; Teronen, Olli; Salo, Tuula; Tjäderhane, Leo

doi:10.1177/00220345010800061301

Cited by 154 publications

(168 citation statements)

References 22 publications

(37 reference statements)

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“…The acidic environment created by bacterial acids can facilitate the activation of endogenous MMPs. Low pH leads to the cleavage of prodomain and thus facilitates the functional activity of MMPs (Tjäderhane et al 1998;Sulkala et al 2001). However, although activated MMPs are stable in acidic pH, they function best at neutral pH.…”

Section: Dental Caries and Role Of Mmps In Caries Progressionmentioning

confidence: 99%

“…CMT-5, a tetracycline analogue with a very low MMP-inhibitory effect compared with CMT-3, had practically no effect of rat molar caries (Tjäderhane et al 1999;Sulkala et al 2001). CMT-3 also eliminates the human salivary gelatinase activity (Sulkala et al 2001), and systemic doxycycline medication has a . MD = mineralized dentin; T = dentinal tubules; A = filled adhesive.…”

Section: Therapeutic Mmp Inhibitors In Caries Progression and Bond Stmentioning

confidence: 99%

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Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases.

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Section: Dental Caries and Role Of Mmps In Caries Progressionmentioning

confidence: 99%

Section: Therapeutic Mmp Inhibitors In Caries Progression and Bond Stmentioning

confidence: 99%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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“…Inhibitors of endogenous dentin proteases other than CHX, such as the quaternary ammonium surface-acting benzalkonium chloride (BAC) 124,125,126,127 the tetracyclines (TCs) and their antimicrobially inactive analogs (minocycline, for now), 71,84,128,129,130 bisphosphonates (batimastat, galardin, and zoled ron ate), 93,129,131,132 g reen tea poly phenol epigallocatechin-3-gallate (EGCG), 40,133,134 and the chelating agent ethylenediaminetetraacetic acid (EDTA) 135,136,137 are being studied as better alternatives associated with dentin hybrid layer preservation.…”

Section: Enzymatic Inhibitorsmentioning

confidence: 99%

Bonding efficiency and durability: current possibilities

et al. 2017

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Bonding plays a major role in dentistry nowadays. Dental adhesives are used in association with composites to solve many restorative issues. However, the wide variety of bonding agents currently available makes it difficult for clinicians to choose the best alternative in terms of material and technique, especially when different clinical situations are considered. Moreover, although bonding agents allow for a more conservative restorative approach, achieving a durable adhesive interface remains a matter of concern, and this mainly due to degradation of the bonding complex in the challenging oral environment. This review aims to present strategies that are being used or those still in development which may help to prevent degradation. It is fundamental that professionals are aware of these strategies to counteract degradation as much as possible. None of them are efficient to completely solve this problem, but they certainly represent reasonable alternatives to increase the lifetime of adhesive restorations.

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“…As the maintenance of the organic matrix is desirable to decrease the progression of erosive dentin lesions 2 , it seems worth to analyse whether the application of potential MMP inhibitors affects indirectly the progression of erosion by reducing the organic breakdown. Previous studies found that the application of potential MMP-inhibitors reduced the dentin collagen solubilisation 10 and diminished the progression of caries lesions in rats 11 . One potent MMP-inhibitor affecting MMP 2, 8 and 9 is chlorhexidine 12 , which was shown to reduce the degradation of the dentin hybrid layer 13 and the solubilisation of dentinal collagen 10 .…”

Section: Introductionmentioning

confidence: 96%

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

Magalhães

Wiegand

Ríos

et al. 2009

Journal of Dentistry

129

121

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OBJECTIVES: This in situ/ex vivo study aimed to analyse the impact of possible MMPinhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. METHODS: Twelve volunteers took part in this cross-over and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoride-free toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60s with: 250 ppm F solution (SnF(2)/AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24, 100% Camellia Sinensis leaf extract, catechin concentration: 30+/-3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (microm). The data were analysed by two-way repeated measures ANOVA and Bonferroni post hoc test. RESULTS: There was a significant difference between the conditions (EroxEro+Abr, p<0.001) and among the solutions (p<0.001). All solutions (F: 1.42+/-0.34; 1.73+/-0.50, chlorhexidine: 1.15+/-0.26; 1.59+/-0.32, green tea: 1.06+/-0.30; 1.54+/-0.55) significantly reduced the dentin wear when compared to control (2.00+/-0.55; 2.41+/-0.83) for both conditions. There were not significant differences among green tea extract, chlorhexidine and F solutions. CONCLUSIONS: Thus, the possible MMP-inhibitors tested in this study seem to be a promising preventive measure to reduce dentin erosion-abrasion, but their mechanism of action needs to be investigated in further studies. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. 2Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ ABSTRACT Objectives: This in situ/ex vivo study aimed to analyse the impact of possible MMP-inhibitors (chlorhexidine and green tea extract) on dentin wear induced by erosion or erosion plus abrasion. Methods:Twelve volunteers took part in this crossover and double-blind study performed in 4 phases of each 5 days. Bovine dentin samples were worn in palatal appliances and subjected to extraoral erosion (4 times/day, Coca-Cola, 5 min) or erosion plus abrasion (2 times/day, fluoridefree toothpaste and electrical toothbrush, 15s/sample). Immediately after each erosion, the appliances were reinserted in the mouth and the oral cavity was rinsed for 60 s with: 250 ppm F solution (SnF 2 /AmF, pH 4.5, Meridol-Gaba, Switzerland), 0.12% chlorhexidine digluconate (0.06% chlorhexidine, pH 6.0, Periogard-Colgate, Brazil), 0.61% green tea extract solution (OM24 ® , 100% Camellia Sinensis leaf extract, catechin concentration:30±3%, pH 7.0, Omnimedica, Switzerland) or deionized water (pH 6.0, control). Dentin loss was assessed by profilometry (µm). The data were analysed by two-way repeated m...

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The Effects of MMP Inhibitors on Human Salivary MMP Activity and Caries Progression in Rats

Cited by 154 publications

References 22 publications

Role of Dentin MMPs in Caries Progression and Bond Stability

Role of Dentin MMPs in Caries Progression and Bond Stability

Bonding efficiency and durability: current possibilities

Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ

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