The Effects of Methylene Blue and Oxygen Concentration on the Photoinactivation of Qβ Bacteriophage

Lee, Denis; Foux, Michael; Leonard, Edward F.

doi:10.1111/j.1751-1097.1997.tb01893.x

Cited by 16 publications

(14 citation statements)

References 23 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several bacteriophages were used in photoinactivation studies as surrogates for mammalian viruses, e.g., MS2 [44], M13 [51,52], PM2 [53], Qβ [54,55,56], PRD1 [57], λ [58,59], φ6 [60], R17 [60], Serratia phage kappa [61], T5 [62], T3 [63], T7 [57,64] and T4-like [65,66,67,68], and the results show that they are effectively photoinactivated.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

Costa

Faustino

Neves

et al. 2012

Viruses

195

205

View full text Add to dashboard Cite

Photodynamic inactivation (PDI) has been used to inactivate microorganisms through the use of photosensitizers. The inactivation of mammalian viruses and bacteriophages by photosensitization has been applied with success since the first decades of the last century. Due to the fact that mammalian viruses are known to pose a threat to public health and that bacteriophages are frequently used as models of mammalian viruses, it is important to know and understand the mechanisms and photodynamic procedures involved in their photoinactivation. The aim of this review is to (i) summarize the main approaches developed until now for the photodynamic inactivation of bacteriophages and mammalian viruses and, (ii) discuss and compare the present state of the art of mammalian viruses PDI with phage photoinactivation, with special focus on the most relevant mechanisms, molecular targets and factors affecting the viral inactivation process.

show abstract

Section: Introductionmentioning

confidence: 99%

Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

Costa

Faustino

Neves

et al. 2012

Viruses

195

205

View full text Add to dashboard Cite

show abstract

“…MB can be activated by irradiation with appropriate wavelength in the red spectral range to an excited state, which in turn activates oxygen to yield oxidising radicals. Such radicals have the phototoxic effect on pathological cells, bacteria and viruses [17][18][19][20][21][22][23]. Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Among the dyes used in photodynamic therapy Methylene Blue (MB) is known widely [17][18][19][20][21][22][23]. It is a commercially available medical dye.…”

Section: Introductionmentioning

confidence: 99%

“…Excitation of the sensitizer by absorption of light of appropriate wavelength in the presence of oxygen converts the sensitizer to its photoactive triple state, which in turn reacts with either a local substrate to form cytotoxic radicals, or with molecular oxygen (02). The reactive oxygen species generated then results in death ofpathological cells, bacteria and viruses [10][11][12][13][14][15][16][17][18][19][20][21][22][23]. Dyes can be applicable for local increase of tissue absorption that is used for precise microsurgery [24], selective laser thermolysis oftumours [25][26][27][28][29][30][31][32][33], treatment ofskin and follicle lesions (for example, acne vulgaris) [34][35][36][37][38], hair removal [10,35,39], etc.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

<title>Methylene blue diffusion in skin tissue</title>

2004

View full text Add to dashboard Cite

The study of Methylene Blue penetration in both skin and subcutaneous fat is presented. Experiments have been carried out with both rat skin and human adipose tissue in vitro at room temperature. Microscopic analysis with digital imaging system has been applied for visualizing and investigation of the Methylene Blue diffusion in the epidermal, dermal and adipose tissue. Diffusion coefficient ofMethylene Blue in skin in vitro has been estimated.

show abstract

“…It inhibits the growth of various organisms such as bacteria (Wainwright 1996;Wainwright et al 1996), yeasts (Peloi et al 2008), parasites (Schirmer et al 2003), and viruses (Lee et al 1997). Its antimicrobial efficiency is enhanced upon illumination with visible light, which indicates involvement of photosensitization mechanism in the MB activity.…”

Section: Introductionmentioning

confidence: 99%

The hybrid methylene blue–zeolite system: a higher efficient photocatalyst for photoinactivation of pathogenic microorganisms

Smolinská

Čı́k

Šeršeň

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

The composite system can be prepared by incorporation of methylene blue (MB) into the channels of zeolite and by adsorption on the surface of the crystals. The composite photosensitizer effectively absorbs the red light (k max = 648 nm) and upon illumination with light-emitting diode at a fluence rate of 1.02 mW cm -2 generates effectively reactive singlet oxygen in aqueous solution, which was proved by EPR spectroscopy. To test efficiency for inactivation of pathogenic microorganisms, we measured photokilling of bacteria Escherichia coli and Staphylococcus aureus and yeasts Candida albicans. We found out that after the microorganisms have been adsorbed at the surface of such modified zeolite, the photogenerated singlet oxygen quickly penetrates their cell walls, bringing about their effective photoinactivation. The growth inhibition reached almost 50 % at 200 and 400 mg modified zeolite in 1 ml of medium in E. coli and C. albicans, respectively. On the other hand, the growth inhibition of S. aureus reached 50 % at far smaller amount of photocatalyst (30 lg per 1 ml of medium). These results demonstrate differences in sensitivities of bacteria and yeast growth. The comparison revealed that concentration required for IC 50 was in case of C. albicans several orders of magnitude lower for a zeolite-immobilized dye than it was for a freely dissolved dye. In S. aureus, this concentration was even lower by four orders of magnitude. Thus, our work suggested a new possibility to exploitation of zeolite and MB in the protection of biologically contaminated environment, and in photodynamic therapy.

show abstract

The Effects of Methylene Blue and Oxygen Concentration on the Photoinactivation of Qβ Bacteriophage

Cited by 16 publications

References 23 publications

Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

Photodynamic Inactivation of Mammalian Viruses and Bacteriophages

<title>Methylene blue diffusion in skin tissue</title>

The hybrid methylene blue–zeolite system: a higher efficient photocatalyst for photoinactivation of pathogenic microorganisms

Contact Info

Product

Resources

About