&lt;title&gt;Methylene blue diffusion in skin tissue&lt;/title&gt;

Genina, Elina A.; Bashkatov, Alexey N.; Tuchin, Valery V.

doi:10.1117/12.572047

Cited by 7 publications

(4 citation statements)

References 37 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Methylene blue (MB) is a well‐known antiseptic, which has a disinfecting and analgesic effect [26‐28]. MB is also one of the photosensitizers for photodynamic therapy (PDT) of fungal diseases [29,30].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Dual‐Stage Method of Active Er:YLF Laser Drug Delivery Through the Nail and Laser‐Induced Transformations of the Drug Extinction Spectrum

2021

View full text Add to dashboard Cite

Background and Objective A novel dual‐stage method for active laser drug delivery (DSLADD) in the treatment of nail diseases is being presented. This method includes sequentially performed microporation of the nail with submillisecond pulses of Er:YLF laser radiation through a layer of an aqueous solution of drug deposited on the nail surface (Stage 1) and exposure this layer to the same laser radiation to deliver drug under the nail plate (Stage 2). The delivery of methylene blue (MB) as one of the possible drugs in the treatment of nail diseases is investigated. The influence of the thickness of the MB layer, as well as the energy and number of applied laser pulses, on the rate of active laser delivery is discussed. To illustrate the possible effect of delivery on the drug delivered, special attention is paid to the deformation of the extinction spectrum of MB solution after laser irradiation. Study Design/Materials and Methods Diode‐pumped Er:YLF laser was used for DSLADD. The process of DSLADD under the nail plate was investigated using digital video microscopy. For different values of the thickness of MB solution layer applied to the nail plate and the energy of laser pulses, the number of laser pulses required to create a single through a microchannel in the nail plate and the number of laser pulses required to deliver the solution to the ventral side of the nail plate after its microporation were registered. The mass and the dose of MB solution penetrated under the nail plate, and the rate of MB solution delivery through a single microchannel was determined. Investigation of the influence of Er:YLF laser radiation parameters on the extinction spectrum of the drug was performed using a fiber spectrometer. The extinction spectra of the 0.001% aqueous solution of MB were recorded before and after exposure to a different number of Er:YLF laser pulses with the energy of 1–4 mJ. Results It was found that the minimum number of laser pulses required for active Er:YLF laser drug delivery under the nail corresponds to the MB layer thickness of 100 μm and the laser pulse energy of 4 mJ. It is shown that in this case, the rate of active laser delivery of MB solution reaches 0.26 ± 0.03 mg/pulse. The radiation of the Er:YLF laser affects the shape of the extinction spectrum of the aqueous solution of MВ, which is associated with the transition of the dye from the monomeric to dimeric state. Depending on the laser pulse energy, the fraction of a certain conformational state in the aqueous MB solution can decrease or increase, stimulating a possible change in its photodynamic and antiseptic activity. Conclusion For the first time, a novel DSLADD through the nail has been described and investigated in vitro. It was demonstrated that at Er:YLF laser pulse repetition rate of f = 30 Hz, microporation of the nail plate and drug delivery through a single microchannel will be about 1.5 s. Lasers Surg. Med. © 2021 Wiley Periodicals LLC

show abstract

Section: Introductionmentioning

confidence: 99%

Investigation of the Dual‐Stage Method of Active Er:YLF Laser Drug Delivery Through the Nail and Laser‐Induced Transformations of the Drug Extinction Spectrum

2021

View full text Add to dashboard Cite

show abstract

“…Although methylene blue labels damaged tissue, the staining intensity within these tissues is heterogeneous. In mammals, excised epidermis displays little methylene blue staining even with very long (hours) staining times as the dye cannot penetrate the stratum corneum (Genina et al, 2004). The epidermis of aquatic axolotls lacks a well-defined stratum corneum (Seifert et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that the thin, newly formed neo-epidermis presents somewhat less of a barrier to methylene blue uptake than more mature, stratified epithelium; this topic awaits more detailed histological analysis. When methylene blue gains access to deeper tissues, it stains nerves (Cook, 1926), vessels, glands, connective tissue fibers, and other acid components of cells and interstitial fluid (Genina et al, 2004). In surgical wound debridement, methylene blue is used to stain the nonviable and granulation tissue (Dorafshar et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Methylene Blue Assay for Estimation of Regenerative Re-EpithelializationIn Vivo

Milyavsky

Dickie

2017

Microsc Microanal

View full text Add to dashboard Cite

The rapidity with which epithelial cells cover a wound surface helps determine whether scarring or scar-less healing results. As methylene blue is a vital dye that is absorbed by damaged tissue but not undamaged epidermis, it can be used to assess wound closure. We sought to develop a quantitative methylene blue exclusion assay to estimate the timeframe for re-epithelialization in regenerating appendages in zebrafish and axolotls, two classic model systems of regeneration. Following application of methylene blue to the amputation plane and extensive washing, the regenerating tail was imaged in vivo until staining was no longer visible. The percent area of the amputation plane positive for methylene blue, representing the area of the amputation plane not yet re-epithelialized, was measured for each time point. The loss of methylene blue occurred rapidly, within ~2.5 h in larval and juvenile axolotls and <1 h in adult zebrafish, consistent with high rates of re-epithelialization in these models of regeneration. The assay allows simple, rapid estimation of the time course for regenerative re-epithelialization without affecting subsequent regenerative ability. This technique will permit comparison of re-epithelialization across different strains and stages, as well as under the influence of various pharmacological inhibitors that affect regeneration.

show abstract

“…MB (M9140, batch MKBR892V, Sigma-Aldrich, Saint-Quentin-Fallavier, France) is a water-soluble non-scattering dye that presents two typical absorption peaks at 609 nm and 668 nm due to dimer and monomer forms in aqueous solutions, respectively [ 37 , 38 , 39 ]. It is commonly used as an absorber for the preparation of liquid optical phantom.…”

Section: Methodsmentioning

confidence: 99%

Discriminating between Absorption and Scattering Effects in Complex Turbid Media by Coupling Polarized Light Spectroscopy with the Mueller Matrix Concept

Ducanchez

Ryckewaert

Heran

et al. 2022

Sensors

View full text Add to dashboard Cite

The separation of the combined effects of absorption and scattering in complex media is a major issue for better characterization and prediction of media properties. In this study, an approach coupling polarized light spectroscopy and the Mueller matrix concept were evaluated to address this issue. A set of 50 turbid liquid optical phantoms with different levels of scattering and absorption properties were made and measured at various orientations of polarizers and analyzers to obtain the 16 elements of the complete Mueller matrix in the VIS–NIR region. Partial least square (PLS) was performed to build calibration models from diffuse reflectance spectra in order to evaluate the potential of polarization spectroscopy through the elements of the Mueller matrix to predict physical and chemical parameters and hence, to discriminate scattering and absorption effects, respectively. In particular, it was demonstrated that absorption and scattering effects can be distinguished in the Rayleigh regime with linear and circular polarization from the M22 and M44 elements of the Mueller matrix, correspondingly.

show abstract

<title>Methylene blue diffusion in skin tissue</title>

Cited by 7 publications

References 37 publications

Investigation of the Dual‐Stage Method of Active Er:YLF Laser Drug Delivery Through the Nail and Laser‐Induced Transformations of the Drug Extinction Spectrum

Investigation of the Dual‐Stage Method of Active Er:YLF Laser Drug Delivery Through the Nail and Laser‐Induced Transformations of the Drug Extinction Spectrum

Methylene Blue Assay for Estimation of Regenerative Re-EpithelializationIn Vivo

Discriminating between Absorption and Scattering Effects in Complex Turbid Media by Coupling Polarized Light Spectroscopy with the Mueller Matrix Concept

Contact Info

Product

Resources

About