The effects of luteinizing hormone as a suppression factor for apoptosis in bovine luteal cells in vitro

Bowolaksono, Anom; Fauzi, Muhammad; Sundari, Ayu Mulia; Pustimbara, Anantya; Lestari, Retno; Abinawanto, .; Dwiranti, Astari; Fadhillah, Fadhillah

doi:10.1111/rda.13913

Cited by 6 publications

(3 citation statements)

References 49 publications

(71 reference statements)

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“…We also found that the number of follicles increased in the diestrus stage after BCR feeding; this was associated with high levels of FSH in the proestrus, producing more follicles [40]. The CL is a temporary endocrine gland formed during ovulation [41]. The addition of PRL inhibited hCG-induced ovulation in a dose-related fashion [42].…”

Section: Effect Of Prl On Follicle Count and CL Numbermentioning

confidence: 99%

The Effect of Prolactin on Gene Expression and the Secretion of Reproductive Hormones in Ewes during the Estrus Cycle

Yue,

Chen,

Duan

et al. 2024

Animals

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Prolactin (PRL) plays an important role in animal follicle development and ovulation. However, its regulatory effects on the different stages of the estrus cycle in ewes are unclear. In this study, bromocriptine (BCR, PRL inhibitor) was used to study the effect of PRL on the secretion of reproductive hormones and gene expressions in order to explore its regulatory effects on the sexual cycle of ewes. Eighty healthy ewes with the same parity and similar weights were randomly assigned to the control group (C, n = 40) and the treatment group (T, n = 40, fed bromocriptine). After estrus synchronization, thirty-one ewes with overt signs of estrus were selected from each group. Six blood samples were randomly obtained by jugular venipuncture to measure the concentration of PRL, estrogen (E2), progesterone (P4), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and gonadotropin-releasing hormone (GnRH) in the proestrus, estrus, metestrus, and diestrus. At the same time, we collected the ovaries of the six ewes in vivo after anesthesia in order to detect follicle and corpus luteum (CL) counts and measure the expression of hormone-receptor and apoptosis-related genes. The results show that PRL inhibition had no significant effects on the length of the estrus cycle (p > 0.05). In proestrus, the number of large and small follicles, the levels of E2, FSH, and GnRH, and the expressions of ER, FSHR, and the apoptotic gene Caspase-3 were increased (p < 0.05); and the number of middle follicles and the expression of anti-apoptotic gene Bcl-2 were decreased (p < 0.05) in the T group. In estrus, the number of large follicles, the levels of E2 and GnRH, and the expressions of the StAR, CYP19A1, and Bcl-2 genes were increased (p < 0.05), and the number of middle follicles was decreased (p < 0.05) in the T group. In metestrus, the number of small follicles and the expression of LHR (p < 0.05) and the pro-apoptotic gene Bax were increased (p < 0.05); the number of middle follicles was decreased (p < 0.05) in the T group. In diestrus, the number of large follicles, middle follicles, and CL, the level of P4, and the expressions of PR, 3β-HSD, StAR, Caspase-3, and Bax were increased (p < 0.05); the number of small follicles and the expression of Bcl-2 were decreased (p < 0.05) in the T group. In summary, PRL inhibition can affect the secretion of reproductive hormones, the follicle count, and the gene expression during the estrus cycle. These results provide a basis for understanding the mechanisms underlying the regulation of the ewe estrus cycle by PRL.

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Section: Effect Of Prl On Follicle Count and CL Numbermentioning

confidence: 99%

The Effect of Prolactin on Gene Expression and the Secretion of Reproductive Hormones in Ewes during the Estrus Cycle

Yue,

Chen,

Duan

et al. 2024

Animals

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“…LH or CG treatment promotes the viability of luteinized granulosa or luteal cells in vitro [ 231 , 262 – 264 ] and protects the CL from regression in vivo [ 265 – 267 ]. The molecular changes that link LHCGR activation to anti-apoptotic effects include upregulation of BCL-2 [ 262 , 267 , 268 ], MCL-1 [ 263 ], survivin [ 269 ], and anti-oxidant enzymes [ 264 ] with simultaneous suppression of BAX and CD95/Fas/Apo-1 [ 262 , 268 ]. The exact mechanisms facilitating these LH/CG effects in the luteal cells are still undetermined, but they may be mediated through PKA-CREB regulation of gene expression [ 258 ].…”

Section: Regulation Of Pcd During Luteolysismentioning

confidence: 99%

A matter of new life and cell death: programmed cell death in the mammalian ovary

Chesnokov,

Mamedova,

Zhivotovsky

et al. 2024

J Biomed Sci

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Background The mammalian ovary is a unique organ that displays a distinctive feature of cyclic changes throughout the entire reproductive period. The estrous/menstrual cycles are associated with drastic functional and morphological rearrangements of ovarian tissue, including follicular development and degeneration, and the formation and subsequent atrophy of the corpus luteum. The flawless execution of these reiterative processes is impossible without the involvement of programmed cell death (PCD). Main text PCD is crucial for efficient and careful clearance of excessive, depleted, or obsolete ovarian structures for ovarian cycling. Moreover, PCD facilitates selection of high-quality oocytes and formation of the ovarian reserve during embryonic and juvenile development. Disruption of PCD regulation can heavily impact the ovarian functions and is associated with various pathologies, from a moderate decrease in fertility to severe hormonal disturbance, complete loss of reproductive function, and tumorigenesis. This comprehensive review aims to provide updated information on the role of PCD in various processes occurring in normal and pathologic ovaries. Three major events of PCD in the ovary—progenitor germ cell depletion, follicular atresia, and corpus luteum degradation—are described, alongside the detailed information on molecular regulation of these processes, highlighting the contribution of apoptosis, autophagy, necroptosis, and ferroptosis. Ultimately, the current knowledge of PCD aberrations associated with pathologies, such as polycystic ovarian syndrome, premature ovarian insufficiency, and tumors of ovarian origin, is outlined. Conclusion PCD is an essential element in ovarian development, functions and pathologies. A thorough understanding of molecular mechanisms regulating PCD events is required for future advances in the diagnosis and management of various disorders of the ovary and the female reproductive system in general.

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“…Increased concentrations of ANGPT1 can lead to reduced inflammation, apoptosis, and fibrosis within tissues [40,41]. Reduction in inflammation and apoptosis is important for reproductive tissues since apoptosis results in a reduction in cell proliferation, follicular atresia, and CL regression [42]. Another important role of ANGPT1 is stabilizing vasculature within cells and tissues [41].…”

Section: Lipid and Cytokine Profiles During Early To Mid-luteal Phasementioning

confidence: 99%

Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle

Snider,

Gomes,

Summers

et al. 2023

Animals

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The process of follicle maturation leading to ovulation is a key milestone in female fertility. It is known that circulating lipids and cytokines play a role in the follicle’s ability to go through follicular maturation and the ovulatory processes. However, the specific mechanisms are not well understood. We posit that dysregulation of granulosa cells influences the ovarian environment, which tries to adapt by changing released lipids and cytokines to achieve follicular maturation. Eleven non-lactating adult females underwent estrus synchronization with two injections of PGF2α 14 days apart. Daily blood samples were collected for 28 days to monitor steroid hormone production after the second injection. To understand the potential impacts of lipids and cytokines during ovulation, a low-dose FSH stimulation (FSHLow) was performed after resynchronization of cows, and daily blood samples were collected for 14 days to monitor steroid hormone production until ovariectomies. The lipidomic analysis demonstrated increased circulating diacylglycerides and triacylglycerides during the mid-luteal phase and after FSHLow treatment. Cholesteryl esters decreased in circulation but increased in follicular fluid (FF) after FSHLow. Increased circulating concentrations of TNFα and reduced CXCL9 were observed in response to FSHLow. Therefore, specific circulating lipids and cytokines may serve as markers of normal follicle maturation.

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The effects of luteinizing hormone as a suppression factor for apoptosis in bovine luteal cells in vitro

Cited by 6 publications

References 49 publications

The Effect of Prolactin on Gene Expression and the Secretion of Reproductive Hormones in Ewes during the Estrus Cycle

The Effect of Prolactin on Gene Expression and the Secretion of Reproductive Hormones in Ewes during the Estrus Cycle

A matter of new life and cell death: programmed cell death in the mammalian ovary

Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle

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