Production of prostaglandins (PGs) and expression of their receptors have been demonstrated in bovine corpus luteum (CL). The aim of the present study was to determine whether PGE2 and PGF2alpha have roles in bovine luteal steroidogenic cell (LSC) apoptosis. Cultured bovine LSCs obtained at the midluteal stage (Days 8-12 of the cycle) were treated for 24 h with PGE2 (0.001-1 microM) and PGF2alpha (0.001-1 microM). Prostaglandin E2 (1 microM) and PGF2alpha (1 microM) significantly stimulated progesterone (P4) production and reduced the levels of cell death in the cells cultured with or without tumor necrosis factor alpha (TNF)/interferon gamma (IFNG), in the presence and absence of FAS ligand (P < 0.05). Furthermore, DNA fragmentation induced by TNF/IFNG was observed to be suppressed by PGE2 and PGF2alpha. Prostaglandin E2 and PGF2alpha also attenuated mRNA expression of caspase 3 and caspase 8, as well as caspase 3 activity (P < 0.05) in TNF/IFNG-treated cells. FAS mRNA and protein expression were decreased only by PGF2alpha (P < 0.05). A specific P4 receptor antagonist (onapristone) attenuated the apoptosis-inhibitory effects of PGE2 and PGF2alpha in the absence of TNF/IFNG (P < 0.05). A PG synthesis inhibitor (indomethacin) reduced cell viability in PGE2- and PGF2alpha-treated cells (P < 0.05). A specific inhibitor of cyclooxygenase (PTGS), PTGS2 (NS-398), also reduced cell viability, whereas an inhibitor of PTGS1 (FR122047) did not affect it. The overall results suggest that PGE2 and PGF2alpha locally play luteoprotective roles in bovine CL by suppressing apoptosis of LSCs.

publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. RESEARCH ARTICLEEvaluation of the implementation of occupational health, safety, and environment management systems in higher education laboratories Laboratory researchers and students may expose to hazardous and toxic chemicals. Implementation of the Occupational Health, Safety, and Environment Management System (OHSEMS) has become a critical aspect in higher education. This study presents an overview of the evaluation of the implementation of the OHSEMS in higher education laboratories. The implementation of the OHSEMS is to prevent occupational accidents in the laboratory. The study design is a semiquantitative descriptive study. The aim of the study is to evaluate the implementation of the OHSEMS in higher education institution laboratories by evaluating the percentages of OHSEMS compliance in higher education laboratories. Five aspects are evaluated: occupational health, safety, and environment (OHSE) policy and commitment, planning, implementation, evaluation, and management review. The result shows that the average compliance with the OHSE policy and commitment aspect is 59.4% and for the planning, implementation, evaluation, and management review, the average compliance percentage are 33.0%, 65.3%, 26.0%, and 0.0%, respectively.

Interleukin-1 (IL-1) is one of the principal cytokines that participate in local regulation of many reproductive functions. The present study was undertaken to determine whether mRNAs for IL-1alpha, IL-1beta, and IL-1 type I receptor (IL-1R) are expressed in bovine corpora lutea (CL), and whether luteal cells respond to treatment with IL-1alpha and IL-1beta during the luteal phase. Bovine CL were classified into five stages (early, Days 2-3; developing, Days 5-6; mid, Days 8-12; late, Days 15-17; and regressed, Days 19-21). IL-1alpha, IL-1beta, and IL-1R mRNAs were detected by reverse transcription-polymerase chain reaction (PCR) in all luteal stages examined. Densitometric analysis of PCR products revealed increases of the mRNA of IL-1alpha and IL-1R in the CL of the regressed stage (P < 0.05). There was less mRNA for IL-1beta in the regressed stage than in the developing and mid stages (P < 0.05). When developing, mid, and late luteal cells were treated with IL-1alpha (1-30 ng/ml) or IL-1beta (1-30 ng/ml) for 24 h, IL-1alpha and IL-1beta dose-dependently increased prostaglandin (PG) F(2alpha) and PGE(2) production by the luteal cells of all stages (P < 0.05), indicating the presence of functional IL-1R in bovine CL. However, progesterone synthesis was not affected by either IL-1alpha or IL-1beta treatment. Stimulation with IL-1alpha and IL-1beta decreased the PGE(2):PGF(2alpha) ratio in the developing stage (P < 0.05), whereas it increased the ratio in the mid stage (P < 0.05). In the late stage, the ratio of IL-1beta-treated cells was greater than that of IL-1alpha-treated cells (P < 0.05). Overall results indicate that genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in the bovine CL throughout the luteal phase, and suggest that IL-1alpha and IL-1beta have different roles as local modulators to regulate PGF(2alpha) and PGE(2) production during the luteal phase.

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The present study was conducted to explore the source of acetylcholine (ACH) in the corpus luteum (CL) and to test our hypothesis of an antiapoptotic role of ACH in the bovine CL and, further, to investigate whether nerve growth factor (NGF), insulin-like growth factor 1 (IGF1), and transforming growth factor beta1 (TGFB1) influence the expression of choline acetyltransferase (CHAT), the biosynthetic enzyme of ACH, in cultured bovine luteal cells. Protein expression and immunolocalization of CHAT were carried out at different stages throughout the luteal phase and in cultured luteal and endothelial cells. ACH was measured in luteal tissue at the different luteal stages and in luteal cells cultured for 8 and 24 h. Cell viability and TUNEL assays were performed on cultured midluteal cells treated with or without tumor necrosis factor alpha (TNF)/interferon gamma (IFNG) in the presence of ACH and its muscarinic (atropine) and nicotinic (mecamylamine) receptor antagonists. The CL was devoid of cholinergic nerve fibers. CHAT immunostaining was evident in luteal, endothelial, and stromal cells in luteal tissue sections and in cultured luteal and endothelial cells. CHAT protein was expressed throughout the cycle without any significant changes. ACH concentration in luteal tissue was not changed during the luteal stages but increased over time and with increased cell numbers in luteal cell cultures. ACH increased cell viability and prevented cell death induced by TNF/IFNG. Atropine significantly attenuated ACH action, whereas mecamylamine had no effect. TNF/IFNG treatment downregulated CHAT expression, whereas NGF, IGF1, and TGFB1 upregulated CHAT expression, in cultured luteal cells. The overall findings strongly suggest a nonneural source and antiapoptotic role of ACH in the bovine CL. Locally produced ACH appears to be regulated by NGF, IGF1, and TGFB1.