The Effects of Insulin and Glucose on Different Characteristics of a UPEC: Alterations in Growth Rate and Expression Levels of some Virulence Genes

Gümüş, Defne; Yörük, Emre; Kalaycı-Yüksek, Fatma; Uz, Gülşen; Topal-Sarikaya, Aysegul; Anğ-Küçüker, Mine

doi:10.7754/clin.lab.2017.170313

Cited by 8 publications

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“…Today it is well known that, the regulation of drug resistance and virulence mechanisms are tightly connected to each other [46]. In accordance with the previous studies our results show that, insulin and glucose can affect the growth and expression levels of different genes related to virulence but also antibiotic resistance [10][11][12]36].…”

Section: Discussionsupporting

confidence: 91%

“…Previous studies suggested that, insulin-r may function as an inter-kingdom QS compound which regulates growth, biofilm formation and chemotactic responses in E. coli [10,11,36].…”

Section: Discussionmentioning

confidence: 99%

“…The mar regulon comprised of some genes as follows:acrA, acrB, marA, marR, ompF and tolC genes [1,9]. During infection, bacteria must withstand stress conditions induced by diverse environmental factors in their host, such as hormones and many other chemicals (sugars, vitamins, ions, pH, bile salts, and microbial metabolites such as indole) which directly affect microbial survival and other biologically important processes, such as growth and gene expression [10]. In several studies, it has been shown that mammalian hormones such asEpinephrine (E), norepinephrine (NE), dopamine, dopa, estrogen, progesterone, serotonin, testosterone, and insulin were shown to affect bacterial growth, gene expression, pathogenicity and antibiotic susceptibility in numerous reports [11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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The effects of insulin and glucose on growth and expression of mar regulon in E. coli

Gümüş

Yüksek

et al. 2019

Turkish Journal of Clinics and Laboratory

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Aim: Host factors are known to modulate virulence, antibiotic susceptibility and growth of bacteria. In the present study we aimed to investigate the effect of human insulin and glucose on growth and expression of mar regulon (marA, marR, ompF, acrA, acrB and tolC) of E. coli SPC105. Material and Methods: E. coli SPC105 was grown in tryptic soy broth (TSB-control) and TSB containing 20 µU/mL insulin, 200 µU/mL insulin, 0.1% glucose and 200 µU/mL insulin + 0.1% glucose. Growths were determined via turbidimetric method. Quantitative polymerase chain reaction (qPCR) was used to determine the gene expression levels. Statistical analysis were performed via Tukey's post hoc-test. Results: According to absorbance values, it has been shown that 0.1% glucose and 200 µU/mL insulin + 0.1% glucose have led a significant decrease on growth. Expression of acrA gene was shown to be decreased in the presence of 200μIU insulin. Expressions of marR and marA were also decreased in 200μU insulin, 0.1 % glucose+200μU insulin and 0.1% glucose added TSB. Decreasing of marA, marR and acrA expressions were shown to be statistically significant (p<0.05). Conclusion: The results of the present study has shown once more that, host factors may influence the growth of a bacterium as well as gene expressions associated with antibiotic susceptibilities.

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Section: Discussionsupporting

confidence: 91%

“…Previous studies suggested that, insulin-r may function as an inter-kingdom QS compound which regulates growth, biofilm formation and chemotactic responses in E. coli [10,11,36].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effects of insulin and glucose on growth and expression of mar regulon in E. coli

Gümüş

Yüksek

et al. 2019

Turkish Journal of Clinics and Laboratory

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“…In addition, a high spread of antibiotic resistance is observed within these pathotypes [7][8][9][10][11][12]. They owe this high capacity to genetic modulate, own pathogenicity and drug resistance [13][14][15]. Several urovirulence factors, such as hemolysin (the hly gene), the cytotoxic necrotizing factor type 1 (the cnf-1 gene), the P-pili (the pap genes), S-family adhesions (the sfa gene), and the bacteriocin usp (the usp gene) are coded by the genes located on pathogenicity islands (PAIs) and through this they can be quickly transferred via the horizontal gene transfer [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains

et al. 2019

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Antibiotic therapy and its consequences in bacterial and human aspects are widely investigated. Despite this, the emergence of new multidrug resistant bacteria is still a current problem. The scope of our work included the observation of changes among uropathogenic Escherichia coli strains after the treatment with a subinhibitory concentration of different antibiotics. The sensitive strains with or without virulence factors were incubated with amoxicillin, ciprofloxacin, gentamycin, or tobramycin. After each passage, the E. coli derivatives were compared to their wild types based on their susceptibility profiles, virulence genes, biofilm formations and the fingerprint profiles of PCR products amplified with using the (N)(6)(CGG)(4) primer. It turned out that antibiotics caused significant changes in the repertoire of bacterial virulence and biofilm formation, corresponding to acquired cross-resistance. The genomic changes among the studied bacteria were reflected in the changed profiles of the CGG-PCR products. In conclusion, the inappropriate application of antibiotics may cause a rapid rise of Multidrug Resistant (MDR) strains and give bacteria a chance to modulate their own pathogenicity. This phenomenon has been easily observed among uropathogenic E. coli strains and it is one of the main reasons for recurrent infections of the urinary tract.

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“…Gümüş ve ark. (47) tarafından bir üropatojen E. coli suşunun üremesinin insülin varlığında değişmediği ancak 200 µU/mL insülin varlığında sfa/foc, cnf1 ve usp gibi virülans genlerinin ekspresyonlarının azaldığı bildirilmiştir.…”

Section: Grafik 6 Farklı Norepinefrin Konsantrasyonlarınınunclassified

Effects of Insulin, Norepinephrine and Estradiol on Growth of Different Microorganisms

Gümüş¹,

Yüksek²,

Uz³

et al. 2018

TMCD

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ÖZ Amaç: Konağa yerleşen mikroorganizmaların üremeleri, virulans ve antibiyotiklere duyarlılık da dâhil olmak üzere tüm özellikleri konak koşulları tarafından kontrol edilir. Çalışmamızda, bir konak faktörü olarak östradiol, insülin ve norepinefrinin farklı mikroorganizmaların (Üropatojen Escherichia coli C7, Candida albicans SC5314, Enterococcus faecalis ATCC 29212, Metisiline dirençli Staphylococcus auerus (MRSA) ATCC 43300 ve Pseudomonas aeruginosa ATCC 27853) üremesi üzerine etkilerinin in vitro olarak belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bu amaçla, mikroorganizmalar hormonların farklı konsantrasyonlarını içeren ve içermeyen (kontrol) besiyerlerinde (Triptik soy buyyon ve Sabouraud dekstroz buyyon) üretilmişlerdir. Mikroorganizmaların üremeleri spektrofotometre ile 4., 6. ve 24. saatlerde ölçülerek belirlenmiştir. Üremelerin istatistiksel değerlendirilmesinde çift yönlü ANOVA Bonferroni post-test kullanılmıştır. Bulgular: Çalışmamızda denenen tüm hormon konsantrasyonlarının 6 saat inkübasyon sonunda E. faecalis üremesini kontrol besiyerine göre istatistiksel olarak anlamlı düzeyde baskıladığı, C. albicans üremesinin hormon ilave edilen besiyerlerinde 24 saat inkübasyonda anlamlı şekilde değiştiği (200 µU/ mL insülin ve 20 pg/mL östradiolda azalma; 150 pg/mL ve 400 pg/mL östradiol ile 1.700 pg/mL, 7.500 pg/mL ve 40.000 pg/mL norepinefrinde artma) belirlenmiştir. Buna karşın denenen hormonların MRSA, P. aeruginosa ve UPEC suşlarının üremesi üzerine herhangi bir etkisi olmadığı görülmüştür (p>0.05). Sonuç: Memeli hormonlarının mikroorganizmaların davranışları üzerinde de etkilerinin gösterilmesi ile konakta çevresel bir unsur olarak enfeksiyon patogenezini belirledikleri vurgulanmaktadır. Çalışmamızdan elde edilen sonuçlar ile farklı konsantrasyonlardaki hormonların mikroorganizmaların üremelerinde çeşitli etkilere sahip olduğu desteklenmektedir. Bu ve benzeri çalışmalar, hormonların insanda enfeksiyon sürecini doğrudan belirlediğini göstermektedir. Anahtar kelimeler: İnter-kingdom haberleşme, insülin, östradiol, norepinefrin, üreme ABSTRACT Effects of Insulin, Norepinephrine and Estradiol on Growth of Different MicroorganismsObjective: All characteristics of microorganisms colonized in host including their growth, virulence and antibiotic susceptibilities are controlled by host conditions. In our study, it has been aimed to determine the effects of estradiol, insulin, norepinephrine as host factors on the growth of various microorganisms (uropathogenic Escherichia coli C7, Candida albicans SC5314, Enterococcus faecalis ATCC 29212, Methicillin resistant Staphylococcus auerus (MRSA) ATCC 43300 and Pseudomonas aeruginosa ATCC 27853) in vitro setting. Material and Methods:For this purpose, microorganisms were grown in different culture media (Tryptic Soy Broth and Sabouraud Dextrose Broth) supplemented with/without various concentrations of hormones. Growths of microorganisms were measured at 4 th , 6 th and 24 th hours using a spectrophotometer. Statistical analysis of growths were determined by using tw...

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The Effects of Insulin and Glucose on Different Characteristics of a UPEC: Alterations in Growth Rate and Expression Levels of some Virulence Genes

Abstract: In the present study, it was shown that insulin and glucose can modulate UPEC's gene expression while the growth rate was not affected.

Cited by 8 publications

References 0 publications

The effects of insulin and glucose on growth and expression of mar regulon in E. coli

The effects of insulin and glucose on growth and expression of mar regulon in E. coli

Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains

Effects of Insulin, Norepinephrine and Estradiol on Growth of Different Microorganisms

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