The Effects of Il-6 and Il-10 and Their Specific Antibodies in the Acute Inflammatory Responses Induced by Carrageenan in the Mouse Model of Pleurisy

Fröde, Tânia Sílvia; Souza, Glória Emília Petto de; Calixto, João B.

doi:10.1006/cyto.2001.0980

Cited by 29 publications

(13 citation statements)

References 37 publications

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“…Injury responsive components such as mast cells, bradykinins and prostaglandins are activated along with the vascular responses and cellular membrane reactions. All of these combined processes and events are represented by the symptoms of edema, inflammation, pain and functional debility [4,11,27].…”

Section: Discussionmentioning

confidence: 99%

“…Previous data has shown that IL-10 is capable of blocking the inflammatory response induced by several inflammatory stimuli in different models [11].…”

Section: Introductionmentioning

confidence: 97%

“…Thus, IL-6 is often used as a marker for systemic activation of pro-inflammatory cytokines [11]. However, evidence also indicates that, under certain conditions, IL-6 has both pro-inflammatory and anti-inflammatory properties [12].…”

Section: Introductionmentioning

confidence: 99%

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Anti‐Inflammatory effects of low‐level laser therapy (660 nm) in the early phase in carrageenan‐induced pleurisy in rat

et al. 2008

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Background and Objective: In the classic model of pleurisy there is little evidence about the anti-inflammatory effects of low-level laser therapy (LLLT) as well the dosage characteristics, such as wavelength, total energy, number and pattern of treatment. In this study we investigated the potential effects of LLLT on modulating the pro-inflammatory and anti-inflammatory mediators of acute inflammation in a rat pleurisy model. Study Design/Materials and Methods: A sample of 48 female Wistar rats were divided into control and experiential groups. An inflammation was induced by carrageenan (0.2 ml) injected into the pleural cavity. At 1, 2, and 3 hours after induction a continuous wave (20 mW) diode laser of the InGaAlP (660 nm) type was used in the four laser groups with different doses and treatment patterns. One group received a single dose of 2.1 J and the other three groups received a total energy of 0.9, 2.1, and 4.2 J. Four hours later the exudate volume, total and differential leukocytes, protein concentration, NO, IL-6, IL-10, TNF-a, and MCP-1 were measured from the aspirated liquid. Results: All the treatment patterns and quantity of energy studied show significant reduction of the exudate volume (P<0.05). Using energy of 0.9 J only NO, IL-6, MCP-1 and IL-10 are significantly reduced (P<0.05). On the other hand, higher energies (2.1 and 4.2 J) significantly reduce all variables independently of the treatment pattern. The neutrophil migration has a straight correlation with the TNF-a (r ¼ 0.551) and NO (r ¼ 0.549) concentration. Conclusions: LLLT-660 nm induced an anti-inflammatory effect characterized by inhibition of either total or differential leukocyte influx, exudation, total protein, NO, IL-6, MCP-1, IL-10, and TNF-a, in a dose-dependent manner. Under these conditions, laser treatment with 2.1 J was more effective than 0.9 and 4.2 J. Lasers Surg.

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Section: Discussionmentioning

confidence: 99%

“…Previous data has shown that IL-10 is capable of blocking the inflammatory response induced by several inflammatory stimuli in different models [11].…”

Section: Introductionmentioning

confidence: 97%

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Anti‐Inflammatory effects of low‐level laser therapy (660 nm) in the early phase in carrageenan‐induced pleurisy in rat

et al. 2008

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“…Carrageenan-induced pleurisy As previously described, 27,28 pleurisy was induced by a single intrapleural injection of 0.1 ml of carrageenan (1%). Since the pleurisy caused by carrageenan exhibits a biphasic response (4 and 48 h), these two interval points were chosen to analyse the studied parameters.…”

Section: Experimental Designmentioning

confidence: 99%

Involvement of steroids in anti‐inflammatory effects of PK11195 in a murine model of pleurisy

Silva

Farges²,

Fröde³

2004

Mediators of Inflammation

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BACKGROUND: Studies on peripheral benzodiazepine receptor function have yielded a diverse list of activities of which the anti-inflammatory effects need to be further examined. AIMS: To evaluate the role of steroids, nitric oxide and adenosine-deaminase in the anti-inflammatory effect of PK11195. METHODS: Pleurisy was induced by intrapleural injection of carrageenan in mice pre-treated or not with PK11195. Leukocytes, exudation, adenosine-deaminase (ADA) activity and nitric oxide (NO) level were measured. Steroid involvement was evaluated by pre-treatment with D,L-aminogluthetimide before PK11195. RESULTS: Leukocytes, exudation and NO levels were reduced by PK11195 in the early (4 h) phase. In the late (48 h) phase, PK11195 decreased leukocytes and ADA activity. D,L-aminogluthetimide reversed the effect of PK11195 on exudate (4 h), as well as total and differential leukocytes and NO levels (48 h). CONCLUSIONS: Steroids, NO and ADA are implicated in the anti-inflammatory action of PK11195.

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“…We chose to use IL-6 as our marker of the inflammatory response because, unlike many other cytokines, its plasma concentrations have been directly correlated with risk of death in intra-abdominal sepsis in humans, and measurements of this single parameter predicted outcome in these cases with remarkable (82.9%) accuracy [18]. Furthermore, although IL-6 is not directly cytotoxic, there is evidence that it modulates other inflammatory cytokines [19,20], and plasma concentrations have been found to be 69% (95% confidence interval 30%-108%) higher in nonsurvivors than in survivors with sepsis [9]. Many studies have shown an association between mean plasma IL-6 concentrations over time and mortality [21], and persistent elevations in IL-6 appear to be more important than initial or peak levels [10].…”

Section: Discussionmentioning

confidence: 99%

Effect of hemofiltration filter adsorption on circulating IL-6 levels in septic rats

Kellum

Dishart

2002

Crit Care

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IntroductionNumerous studies have shown that hemofiltration using porous synthetic membranes can and does remove a wide range of substances that mediate inflammation from the plasma [1][2]. Limited evidence supports the notion that this treatment can also influence circulating plasma concentrations of various mediators [3][4][5] and mounting evidence suggests that these manipulations have important biologic effects [6][7][8]. Despite these advances, there are no randomized clinical trials demonstrating that hemofiltration improves outcome in patients with sepsis. Furthermore, the exact mechanisms responsible for immunomodulation with hemofiltration remain uncertain. Modern hemofiltration filters may adsorb mediator substances as well as permitting them to pass through the membrane. Determining which of these mechanisms is dominant will be essential to advancing the design of materials and methodology suited to this form of therapy. If adsorption is the primary effect, the surface area of the filters must be ANOVA = one-way analysis of variance; AUC = area under the curve; CLP = cecal ligation and puncture; ELISA = enzyme-linked immunosorbent assay; IL = interleukin; SNK = Student-Newman-Keuls; TNF = tumor necrosis factor. AbstractIntroduction Hemofiltration may modulate the inflammatory response in sepsis through a variety of mechanisms. We sought to distinguish clearance from adsorption as the principal mechanism responsible for reducing circulating IL-6 levels with hemofiltration. Materials and methods Nine hours after cecal ligation and puncture in 18 adult male SpragueDawley rats, we divided the rats into three groups (6 animals each) and placed groups 2 and 3 on a hemofiltration circuit connected between the right carotid artery and femoral vein using an AN69 membrane. In the hemofiltration group (group 2), ultrafiltrate was replaced with lactated Ringer's solution; in the recirculation group (group 3), the ultrafiltrate was reinfused into the animal. A sham group (group 1) had an arteriovenous circuit inserted but no hemofiltration. Blood was obtained for measurement of IL-6 and tumor necrosis factor (TNF) at the start of hemofiltration and after 5 and 11 hours of treatment. Results and discussion IL-6 levels increased only in the sham-treated animals (20.4 ± 11.3 at baseline to 62.3 ± 16.8 pg/ml at 11 hours, P = 0.03) (differences between groups 1 and 2, P = 0.015, and groups 1 and 3, P = 0.028). TNF levels were highly variable but not significantly different among the three groups. Conclusion Hemofiltration-associated reductions in circulating IL-6 levels appear to be secondary to adsorption of mediators to the filter membrane. We do not know whether this is due to direct adsorption of IL-6 per se or to the absorption of other mediators with secondary downregulation of IL-6 production or release. In addition, we could not exclude an interaction between adsorption and hemofiltration.

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The Effects of Il-6 and Il-10 and Their Specific Antibodies in the Acute Inflammatory Responses Induced by Carrageenan in the Mouse Model of Pleurisy

Cited by 29 publications

References 37 publications

Anti‐Inflammatory effects of low‐level laser therapy (660 nm) in the early phase in carrageenan‐induced pleurisy in rat

Anti‐Inflammatory effects of low‐level laser therapy (660 nm) in the early phase in carrageenan‐induced pleurisy in rat

Involvement of steroids in anti‐inflammatory effects of PK11195 in a murine model of pleurisy

Effect of hemofiltration filter adsorption on circulating IL-6 levels in septic rats

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