The Effects of Hypoxia on U937 Cell Line in Mesenchymal Stem Cells Co-Culture System

Ejtehadifar, Mostafa; Shamsasenjan, Karim; Akbarzadehlaleh, Parvin; Zahedi, Sara; Kazemi, Narjes

doi:10.15171/apb.2016.079

Cited by 9 publications

(7 citation statements)

References 36 publications

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“…The influence of controlled oxygen exposure when culturing MSCs in vitro has been largely studied [96][97][98]. Normally, in vitro cell culture is carried out under an 18-21% oxygen concentration.…”

Section: Hypoxiamentioning

confidence: 99%

Mesenchymal Stromal Cell Secretome for the Treatment of Immune-Mediated Inflammatory Diseases: Latest Trends in Isolation, Content Optimization and Delivery Avenues

et al. 2021

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Considering the high prevalence and the complex pharmacological management of immune-mediated inflammatory diseases (IMIDs), the search for new therapeutic approaches for their treatment is vital. Although the immunomodulatory and anti-inflammatory effects of mesenchymal stromal cells (MSCs) have been extensively studied as a potential therapy in this field, direct MSC implantation presents some limitations that could slow down the clinical translation. Since the beneficial effects of MSCs have been mainly attributed to their ability to secrete a plethora of bioactive factors, their secretome has been proposed as a new and promising pathway for the treatment of IMIDs. Formed from soluble factors and extracellular vesicles (EVs), the MSC-derived secretome has been proven to elicit immunomodulatory effects that control the inflammatory processes that occur in IMIDs. This article aims to review the available knowledge on the MSC secretome, evaluating the advances in this field in terms of its composition, production and application, as well as analyzing the pending challenges in the field. Moreover, the latest research involving secretome administration in IMIDs is discussed to provide an updated state-of-the-art for this field. Finally, novel secretome delivery alternatives are reviewed, paying special attention to hydrogel encapsulation as one of the most convenient and promising strategies.

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Section: Hypoxiamentioning

confidence: 99%

Mesenchymal Stromal Cell Secretome for the Treatment of Immune-Mediated Inflammatory Diseases: Latest Trends in Isolation, Content Optimization and Delivery Avenues

et al. 2021

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“…At the end, MTT assay was performed as described in the previous section. According to our previous work, we expected that hBM‐MSC‐CM have no effect on U937 proliferation rate, but we observed that both hBM‐MSCs and hBM‐MSC‐CM induce proliferation of U937 cells, even in the presence of SEB. Importantly, here we showed that solid and soluble factors which were produced by hBM‐MSCs are involved in this finding.…”

Section: Resultsmentioning

confidence: 61%

“…MTT assay results compares proliferation rate of control group (U937 cells in RPMI-1640) with cultured U937 cells with different number of hBM-MSCs, 10 4 cells/well was determined as the optimum number of hBM-MSCs (n = 3); Bars, SEM. *P < .05 is considered significant, using one-way ANOVA on U937 proliferation rate, 30 but we observed that both hBM-MSCs and hBM-MSC-CM induce proliferation of U937 cells, even in the presence of SEB. Importantly, here we showed that solid and soluble factors which were produced by hBM-MSCs are involved in this finding.…”

Section: The Hbm-mscs and CM Decreased The Suppressive Effect Of Sementioning

confidence: 78%

Bone marrow ‐ mesenchymal stem cells impact on the U937 cells in the presence of staphylococcal enterotoxin B (SEB)

Ejtehadifar

Halabian

Ghazavi

et al. 2018

Clin Exp Pharma Physio

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The growing resistance against conventional chemotherapy in acute myeloid leukemia (AML) is a noticeable clinical concern. Therefore, many researchers are looking for novel substances to overcome drug resistance in cancer. Staphylococcal enterotoxin B (SEB) is a superantigen (SAg) and a promising compound which has lethal effects on malignant cells. In this unprecedented study, SEB was used against U937 cells in a co-culture system in the presence of human bone marrow-mesenchymal stem cells (hBM-MSCs). The effects of hBM-MSCs on the proliferation and survival of U937 cell line with SEB was assessed using MTT assay and AnnexinV/PI flowcytometry, respectively. Moreover, the expression of IL-6, IL-10, TGF-β, and inhibitor of nuclear factor kappa-B kinase (IKKb) was evaluated by real-time PCR technique. The same experiments were also carried out using hBM-MSCs-conditioned medium (hBM-MSCs-CM). The results showed that SEB reduced the proliferation and survival of U937 cell line, but hBM-MSCs or hBM-MSCs-CM suppressed the effects of SEB. Furthermore, real-timePCR demonstrated that SEB could decrease the expression of IL-6, IL-10, and TGF-β in hBM-MSCs (P < .05), while the production of IKKb was increased in comparison with the control group. These findings help us to have a broader understanding ofthe usage of SEB in the treatment of haematological malignancies, especially if it is targeted against hBM-MSCs to disrupt their supportive effects on malignant cells.

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“…Similarly, MSCs obtained from patients with leukemia have been shown to attenuate the proliferative capacity of leukemia cells in vitro, leading to reduced cell survival 14,15 . On the other hand, these cells could modify the leukemia cells' biological reactions such as apoptosis in both normoxic and hypoxic conditions 13,16 …”

Section: Introductionmentioning

confidence: 99%

“…14,15 On the other hand, these cells could modify the leukemia cells' bio-logical reactions such as apoptosis in both normoxic and hypoxic conditions. 13,16 Concerning the recent studies, it has been proven that tumor cells have a high survival rate, high chemotherapy resistance, and more proliferation capacity under low oxygen (hypoxic) conditions. [17][18][19] In the hypoxic environment, distinct biologic reactions occur at the transcriptional, translational, or posttranslational levels in normal/tumor cells, 20,21 while some key genes involved in cellular functions are suppressed at the transcriptional level under the hypoxic environment.…”

Section: Introductionmentioning

confidence: 99%

Under hypoxic conditions, MSCs affect the expression and methylation level of survival‐related genes in ALL independent of apoptosis pathways in vitro

Marofi

Shomali

Younus³

et al. 2021

Biotech and App Biochem

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Mesenchymal stem cells (MSCs) are one of the most prominent cells in the bone marrow. MSCs can affect acute lymphocytic leukemia (ALL) cells under hypoxic conditions. With this aim, we used MOLT-4 cells as simulators of ALL cells cocultured with bone marrow mesenchymal stem cells (BMMSCs) under hypoxic conditions in vitro. Then, mRNA and protein expression of the MAT2A, PDK1, and HK2 genes were evaluated by real-time PCR and Western blot which was also followed by apoptosis measurement by a flow-cytometric method. Next, the methylation status of the target genes was investigated by MS-qPCR. Additionally, candidate gene expressions were examined after treatment with rapamycin using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. We

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The Effects of Hypoxia on U937 Cell Line in Mesenchymal Stem Cells Co-Culture System

Cited by 9 publications

References 36 publications

Mesenchymal Stromal Cell Secretome for the Treatment of Immune-Mediated Inflammatory Diseases: Latest Trends in Isolation, Content Optimization and Delivery Avenues

Mesenchymal Stromal Cell Secretome for the Treatment of Immune-Mediated Inflammatory Diseases: Latest Trends in Isolation, Content Optimization and Delivery Avenues

Bone marrow ‐ mesenchymal stem cells impact on the U937 cells in the presence of staphylococcal enterotoxin B (SEB)

Under hypoxic conditions, MSCs affect the expression and methylation level of survival‐related genes in ALL independent of apoptosis pathways in vitro

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