The effects of hydro-ethanolic extract of Capparis spinosa (C. spinosa) on lipopolysaccharide (LPS)-induced inflammation and cognitive impairment: Evidence from in vivo and in vitro studies

Rahimi, Vafa Baradaran; Rajabian, Arezoo; Rajabi, Hamed; Vosough, Elahe Mohammadi; Mirkarimi, Hamid Reza; Hasanpour, Maede; Iranshahi, Mehrdad; Rakhshandeh, Hassan; Askari, Vahid Reza

doi:10.1016/j.jep.2020.112706

Cited by 39 publications

(52 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Bradford protein assay was carried out to quantify the total protein concentration in a sample using the Coomassie Brilliant Blue G-250 dye [ 24 – 27 ]. First, the dye (10 mg) was dissolved in 50 ml of ethanol (96%), and then phosphoric acid (85%) (10 ml) was added, and the volume of the solution reached 100 ml.…”

Section: Methodsmentioning

confidence: 99%

“…Then, after sample pouring (20 μ l), a Bradford reagent (200 μ l) was added to the 96-well microplate. Finally, after 5 minutes, the absorption was read out at 595 nm with a microplate reader [ 24 – 27 ].…”

Section: Methodsmentioning

confidence: 99%

“…The ELISA assay evaluated the IL-1 β , IL-6, TNF- α , and PGE2 levels as inflammatory mediators, which were carried out in accordance with the manufacturers' protocol, IBL company [ 6 , 7 , 24 , 26 , 28 ], USA. In summary, 1.5 × 10 6 cells were cultured in a 6-well plate overnight, and they were treated according to the experimental grouping section.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells

Taherzadeh

Rahimi

Amiri

et al. 2022

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Acetyl-11-keto-beta-boswellic acid (AKBA), the major component of Boswellia serrata, exhibits anti-inflammatory activities. This in vitro study investigated the protective effects of AKBA against lipopolysaccharide (LPS)-induced cardiac dysfunction. In this study, the H9C2 cardiomyocytes were pretreated with AKBA (2.5, 5, and 10 μM for 24 h), and then cotreated with LPS for another 24 h. The MTT assay, ELISA test kits, and quantitative real-time PCR analysis assessed the cell viability, levels of proinflammatory factors (IL-β, IL-6, TNF- α, and PGE2), and the gene expression of IL-β, IL-6, TNF- α, iNOS, and COX-2, respectively. The nitric oxide (NO) and thiol levels were also measured using a biochemical assay. The results indicated that LPS exposure markedly reduced cell viability and total thiol content, but increased the inflammatory cytokines, NO metabolites, and gene expression of proinflammatory mediators in H9C2 cells. AKBA pretreatment significantly altered the mentioned factors induced by LPS. Our results demonstrated that AKBA might be a promising therapeutic agent for treating sepsis-related cardiac dysfunction in the future.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells

Taherzadeh

Rahimi

Amiri

et al. 2022

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…These inflammatory cytokines play an essential role in the development of peritoneal adhesion. Transforming growth factor-beta (TGF- β ) is expressed by adhesion fibroblasts and mesothelial cells, which lead to adhesion formation and fibrosis [ 7 , 10 ]. Additionally, vascular endothelial growth factor (VEGF) is another important marker in angiogenesis, wound healing, and adhesion formation [ 2 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Intraperitoneal Lavage with Crocus sativus Prevents Postoperative‐Induced Peritoneal Adhesion in a Rat Model: Evidence from Animal and Cellular Studies

Rahmanian-Devin

Rakhshandeh

Rahimi

et al. 2021

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Postoperative peritoneal adhesions are considered the major complication following abdominal surgeries. The primary clinical complications of peritoneal adhesion are intestinal obstruction, infertility, pelvic pain, and postoperative mortality. In this study, regarding the anti-inflammatory and antioxidant activities of Crocus sativus, we aimed to evaluate the effects of Crocus sativus on the prevention of postsurgical-induced peritoneal adhesion. Male Wistar-Albino rats were used to investigate the preventive effects of C. sativus extract (0.5%, 0.25% and 0.125% w / v ) against postsurgical-induced peritoneal adhesion compared to pirfenidone (PFD, 7.5% w / v ). We also investigated the protective effects of PFD (100 μg/ml) and C. sativus extract (100, 200, and 400 μg/ml) in TGF-β1-induced fibrotic macrophage polarization. The levels of cell proliferation and oxidative, antioxidative, inflammatory and anti-inflammatory, fibrosis, and angiogenesis biomarkers were evaluated both in vivo and in vitro models. C. sativus extract ameliorates postoperational-induced peritoneal adhesion development by attenuating oxidative stress [malondialdehyde (MDA)]; inflammatory mediators [interleukin- (IL-) 6, tumour necrosis factor- (TNF-) α, and prostaglandin E2 (PGE2)]; fibrosis [transforming growth factor- (TGF-) β1, IL-4, and plasminogen activator inhibitor (PAI)]; and angiogenesis [vascular endothelial growth factor (VEGF)] markers, while propagating antioxidant [glutathione (GSH)], anti-inflammatory (IL-10), and fibrinolytic [tissue plasminogen activator (tPA)] markers and tPA/PAI ratio. In a cellular model, we revealed that the extract, without any toxicity, regulated the levels of cell proliferation and inflammatory (TNF-α), angiogenesis (VEGF), anti-inflammatory (IL-10), M1 [inducible nitric oxide synthase (iNOS)] and M2 [arginase-1 (Arg 1)] biomarkers, and iNOS/Arg-1 ratio towards antifibrotic M1 phenotype of macrophage, in a concentration-dependent manner. Taken together, the current study indicated that C. sativus reduces peritoneal adhesion formation by modulating the macrophage polarization from M2 towards M1 cells.

show abstract

“…This plant is native to the Mediterranean and is compatible with dry climate, and also grows widely in west and central Asia like Iran and Armenia (Samari et al 2019;Motamedi et al 2020). It is used economically due to applications of its flower buds and fruits, and in cuisine and traditional medicine usages of various parts of the plant (seeds, aerial parts, and roots) for different ailments such as rheumatism, gout, and pain treatment (Tir et al 2019, Baradaran Rahimi et al 2020. It is essential to declare that Capparis seeds contain ~ 30-50% oil in which oleic, linoleic, and palmitic acids are the dominant unsaturated fatty acids (Matthäus and Özcan 2005).…”

Section: Introductionmentioning

confidence: 99%

Parametric optimization of biodiesel synthesis from Capparis spinosa oil using NaOH/NaX as nanoheterogeneous catalyst by response surface methodology

Helmi

Tahvildari

Hemmati

2020

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

In this research, Capparis spinosa oil was used as a feedstock for biodiesel production in the presence of NaOH/NaX as a nano heterogeneous catalyst. The NaOH/NaX catalyst was characterized by XRD, SEM, and BET analyses. The transesterification reaction was optimized through response surface methodology (RSM) based on the central composite design (CCD) of experiments. The effects of key variables of catalyst weight, methanol to oil molar ratio, and time on the reaction were studied, and a precise discussion about the impact of each process variable on the biodiesel yield was carried out. The highest biodiesel yield was 90.33% for the optimum reaction conditions that include a constant temperature of 60 °C, methanol to oil molar ratio of 6.7:1, catalyst weight of 2.3 wt%, and reaction time of 1.24 h. Furthermore, all physicochemical properties of the produced biodiesel were matched to the ASTM standard. Based on the obtained results, Capparis spinosa oil is a suitable source for biodiesel production.

show abstract

The effects of hydro-ethanolic extract of Capparis spinosa (C. spinosa) on lipopolysaccharide (LPS)-induced inflammation and cognitive impairment: Evidence from in vivo and in vitro studies

Cited by 39 publications

References 58 publications

Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells

Acetyl-11-Keto-β-Boswellic Acid (AKBA) Prevents Lipopolysaccharide-Induced Inflammation and Cytotoxicity on H9C2 Cells

Intraperitoneal Lavage with Crocus sativus Prevents Postoperative‐Induced Peritoneal Adhesion in a Rat Model: Evidence from Animal and Cellular Studies

Parametric optimization of biodiesel synthesis from Capparis spinosa oil using NaOH/NaX as nanoheterogeneous catalyst by response surface methodology

Contact Info

Product

Resources

About