The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars

Chen, Bin; Sun, Hao; Wang, Hanguo; Kong, Han-Bae; Chen, Fa‐Ming; Yu, Qing

doi:10.1016/j.biomaterials.2012.03.057

Cited by 80 publications

(90 citation statements)

References 53 publications

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“…These include autologous or allogenic human serum, 38 human plasma, 37 and human platelet lysates and their released factors. 4 Pisciolaro and collaborators showed that autologous human serum induces higher CFUs capacity, higher degree of mineralization and less cell damage in DPSCs cells then FBS and allogenic human serum. 38 Pisciotta and collaborators showed that human serum is an appropriate alternative for FCS, enhancing DPSC proliferation rate and improving in vitro differentiation, and when implanted in immunocompromised rats these cells can restore critical size bone defects.…”

Section: Discussionmentioning

confidence: 99%

“…37 Chen and collaborators showed that when 5% of human platelet lysate was incorporated in normal culture medium the proliferation and mineralization rates were enhanced. 4 Although the literature refers to human platelets as a substitute for FBS, some articles used them in association with FBS, more likely as supplement.…”

Section: Discussionmentioning

confidence: 99%

“…2 This discovery provided an important source of stem cells, since DPSCs can be obtained by a minimally invasive process: 3 from teeth extracted due to orthodontic reasons, periodontal disease or caries. 4 Furthermore, DPSCs have shown high proliferative capacity, maintaining the potential of self-renewal and differentiation into multiple cell lineages. 2 Several studies have considered DPSCs an appropriate cell model for various applications in different health-related fields.…”

Section: Introductionmentioning

confidence: 99%

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How has dental pulp stem cells isolation been conducted? A scoping review

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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How has dental pulp stem cells isolation been conducted? A scoping review

et al. 2017

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“…MSCs are a group cells that have been isolated from virtually every vascularized tissue [62]. MSCs are a group cells that have been isolated from virtually every vascularized tissue [52]; however, recent reports have documented that they can also be isolated from other sources as umbilical cord [62], peripheral blood [63], adipose tissue [64][65][66][67], hair follicle [68], periodontal ligament [69][70][71][72], gingival tissue [73] and dental pulp [74,75], among others.…”

Section: Mesenchymal Stem Cells-based Therapymentioning

confidence: 99%

Regenerative Medicine: A New Paradigm in Bone Regeneration

Chaparro¹,

Linero²

2016

Advanced Techniques in Bone Regeneration

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Bone defects are the cause of functional disability and the restoration of skeletal function remains an important challenge on orthopedics, neurosurgery and oral and maxillofacial surgery. Because of the limitations of the currently used techniques for the reconstruction of bone defects and the difficulties for the implementation of new therapeutic strategies, a new paradigm in the field of reconstructive surgery has arisen, leading to tissue engineering and regenerative medicine. Mesenchymal stem cells (MSC) have emerged as a promising alternative for the treatment of bone lesions. It was postulated that the therapeutic action was the result of proliferation and differentiation of MSCs, replacing injured tissue. However, recent studies have shown that MSCs secrete a number of trophic factors that have a strong effect during repair and tissue regeneration. This represents a shift from a paradigm centered on MSC proliferation and differentiation to a new paradigm in which the MSCs exert their beneficial effect by the secretion of paracrine factors that induce endogenous repair mechanisms. This chapter will bring together basic and clinical aspects, focused on novel findings on MSC paracrine effect and the development of new therapeutic strategies based on growth factors, cytokines and signaling molecules involved in bone regeneration.

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“…Mesenchymal stem cells (MSC) from bone marrow can be isolated and expanded through several passages while retaining their multipotent differentiation capacity 1,2) . However, bone marrow harvesting is invasive, and many alternative sources of MSC are also considered to be potentially useful in tissue engineering, including adipose tissue 3,4) and dental pulp 5,6) . Considerable attention has been paid to human dental follicle cells (hDFC) that originate in the dental follicle, which is a loose ectomesenchymally derived connective tissue surrounding the enamel organ and the dental papilla of the developing tooth germ prior to eruption 7) .…”

Section: Introductionmentioning

confidence: 99%

Osteogenic Potential of Human Dental Follicle Cells on Rat Calvaria

Iwai

Kuyama

Kuboyama

et al. 2013

J. Hard Tissue Biology.

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The objective of this study was to investigate the osteogenic potential of human dental follicle cells (hDFC) in vivo. hDFC were isolated from dental follicle tissue by enzymatic digestion and cultured in growth medium (GM). hDFC were grown in a three-dimensional (3D) culture using gelatin sponges in osteogenic induction medium (OIM) or GM. The cells were transplanted onto calvaria bone of immunodeficient rats (n = 3). Hematoxylin and eosin (H-E) staining and immunohistochemistry were performed, and bone formation was analyzed with micro-computed tomography (micro-CT). H-E staining showed newly formed bone after transplantation of hDFC grown in a 3D culture in OIM. Immunohistochemistry showed BMP-2, Runx2, and Osterix expression in the hDFC transplantation site during the early stage of bone formation. Moreover, micro-CT showed that the transplanted hDFC that were 3D-cultured in OIM promoted good bone quality and bone volume compared to the other two groups. Thus, human dental follicles may be a potentially useful cell source for regenerative therapy.

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The effects of human platelet lysate on dental pulp stem cells derived from impacted human third molars

Cited by 80 publications

References 53 publications

How has dental pulp stem cells isolation been conducted? A scoping review

How has dental pulp stem cells isolation been conducted? A scoping review

Regenerative Medicine: A New Paradigm in Bone Regeneration

Osteogenic Potential of Human Dental Follicle Cells on Rat Calvaria

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