The Effects of Honey on Leishmania Parasites: An <i>In Vitro</i> Study

Zeina, B.; Bi, Zohra; Al-Assad, Saada

doi:10.1177/00494755970270s112

Cited by 23 publications

(4 citation statements)

References 7 publications

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“…Consistent with our study, honey incorporated with hydroalcoholic extract of Nigella sativa was found effective against Leishmania parasite [ 24 ]. Other studies reported the same results as in our study [ 28 , 33 , 47 ].…”

Section: Discussionsupporting

confidence: 92%

Evaluating leishmanicidal effects of Lucilia sericata products in combination with Apis mellifera honey using an in vitro model

Sherafati¹,

Dayer²,

Ghaffarifar³

et al. 2023

PLoS ONE

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Leishmaniasis is a zoonotic disease caused by an intracellular parasite from the genus Leishmania. Lack of safe and effective drugs has increasingly promoted researches into new drugs of natural origin to cure the disease. The study, therefore, aimed to investigate the anti-leishmanial effects of Lucilia sericata larval excretion/secretion (ES) in combination with Apis mellifera honey as a synergist on Leishmania major using an in vitro model. Various concentrations of honey and larval ES fractions were tested against promastigotes and intracellular amastigotes of L. major using macrophage J774A.1 cell line. The inhibitory effects and cytotoxicity of ES plus honey were evaluated using direct counting method and MTT assay. To assess the effects of larval ES plus honey on the amastigote form, the rate of macrophage infection and the number of amastigotes per infected macrophage cell were estimated. The 50% inhibitory concentration (IC50) values were 21.66 μg/ml, 43.25 60 μg/ml, 52.58 μg/ml, and 70.38 μg/ml for crude ES plus honey, ES >10 kDa plus honey, ES <10 kDa plus honey, and honey alone, respectively. The IC50 for positive control (glucantime) was 27.03 μg/ml. There was a significant difference between viability percentages of promastigotes exposed to different doses of applied treatments compared to the negative control (p≤ 0.0001). Microscopic examination of amastigote forms revealed that dosages applied at 150 to 300 μg/ml significantly reduced the rate of macrophage infection and the number of amastigotes per infected macrophage cell. Different doses of larval products plus honey did not show a significant toxic effect agaist macrophage J774 cells. The larval ES fractions of L. sericata in combination with A. mellifera honey acted synergistically against L. major.

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Section: Discussionsupporting

confidence: 92%

Evaluating leishmanicidal effects of Lucilia sericata products in combination with Apis mellifera honey using an in vitro model

Sherafati¹,

Dayer²,

Ghaffarifar³

et al. 2023

PLoS ONE

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“…In a study, it was determined that 10% honey concentration applied to the Ecinococcus granulosus parasite, which causes hydatid cyst (echinococcosis), has a lethal effect from the third minute (Karadal & Yildirim, 2012). Antileishmanial property of honey is observed in some studies (Bassam, 1997). In a study conducted to determine the antimicrobial effects of honey samples collected from Bingöl region, it was stated that 0.1 mL honey sample inhibits the growth of bacterial species such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Bacillus brevis, Pseudomonas aeroginosa and fungi species such as Candida albicans and Rhodotorula rubra (Aksoy & Digrak, 2006).…”

Section: Antimicrobial Property Of Honeymentioning

confidence: 99%

A Review about Honey Effect on Human Body

ÇOŞKUN

Çoşkun

2022

Journal of Apitherapy and Nature

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Bal, birçok hastalık için tedavi olarak kullanılan ve halen beslenmenin vazgeçilmez bir parçası olan geleneksel bir besindir. Balın besleyici özelliklerinin yanı sıra kanserden, nörolojik ve kardiyovasküler hastalıklardan, enfeksiyonlardan korunma; gastrointestinal sistemin korunması; bağışıklık sistemini güçlendirmek; bazı ameliyat sonrası komplikasyonları hafifletmek ve hormonal seviyeleri düzeltmek. Bu çalışmada, balın her bir sistem üzerindeki etkilerini inceleyen insan kontrollü denemelere ek olarak in vivo ve in vitro deneyleri içeren araştırmalara yer verilmiştir. Bu çalışmaların sonuçları ile geleneksel uygulamalar arasında bağlantı kurulması amaçlanmıştır. Yapılan araştırmalar balın faydalarını genel hatlarıyla açıklamaya yetse de konuyla ilgili daha kontrollü deneme örneklerine ihtiyaç duyulmaktadır. Bu çalışmada aynı zamanda tıbbi standardize edilmiş ballar hakkında erişilebilirliği ve farkındalığı teşvik etmek de amaçlandı. Bunların sağlanabilmesi için toplumu bilgilendirmenin yanı sıra güvenli bal üretimi ve paketleme konusunda üreticiler uyarılmalıdır.

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“…67,68 Honey has been reported to be effective in the treatment of Candida albicans, cutaneous leishmaniasis, and rubella virus. 43,69,70 Honey may be helpful for labial and genital herpes simplex virus lesions. 71 When compared with topical acyclovir, multifloral natural honey applied 4 times a day healed faster (5.85 Ϯ 1.57 days for acyclovir compared with 2.57 Ϯ 0.95 days with honey; P o .05, n ¼ 16), with less pain and less crusting.…”

Section: Other Usesmentioning

confidence: 99%

Wound Care in the Wilderness: Is There Evidence for Honey?

Stewart

McGrane

Wedmore

2014

Wilderness & Environmental Medicine

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Honey is one of the most ancient remedies for wound care. Current research has shown promising results for its use in wound care. This review is intended to inform readers of the physiological properties of honey and the evidence that exists to support its clinical use. When compared with evidence for current wound treatment, honey has proven to be a safe, effective, and sometimes superior treatment for various wounds. There are currently US Food and Drug Administration–approved medical-grade honey products available in the United States. Although there have been no clinical trials exploring the use of honey in wilderness environments, it may be a safe, improvisational wound treatment. More robust studies are needed for definitive conclusions of its efficacy and safety.

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The Effects of Honey on Leishmania Parasites: An In Vitro Study

Abstract: The activities of honey dilutions were investigated against three species of leishmania. The results were compared with the effects of the same concentrations of sugar. Honey and sugar both have anti-leishmanial effects in vitro, but our results indicated that honey is superior to sugar.

Cited by 23 publications

References 7 publications

Evaluating leishmanicidal effects of Lucilia sericata products in combination with Apis mellifera honey using an in vitro model

Evaluating leishmanicidal effects of Lucilia sericata products in combination with Apis mellifera honey using an in vitro model

A Review about Honey Effect on Human Body

Wound Care in the Wilderness: Is There Evidence for Honey?

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