The effects of high magnitude cyclic tensile load on cartilage matrix metabolism in cultured chondrocytes

Honda, K.; Ohno, Shigeru; Tanimoto, Kotaro; Ijuin, Chise; Tanaka, Nobuaki; Doi, Takumi; Kato, Yukio; Tanne, Kazuo

doi:10.1078/0171-9335-00089

Cited by 139 publications

(132 citation statements)

References 51 publications

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“…al., 2004, MacLean et al, 2005. In cell culture models, cyclic stretching has been shown to up-regulate MMP-3 expression in chondrocytes (Honda et al, 2000) and tendon cells (Tzusaki et al, 2003). Mechanical load has also been found to have an inhibitory effect.…”

Section: Discussionmentioning

confidence: 99%

Cyclic loading inhibits expression of MMP-3 but not MMP-1 in an in vitro rabbit flexor tendon model

Asundi

Rempel

2008

Clinical Biomechanics

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Section: Discussionmentioning

confidence: 99%

Cyclic loading inhibits expression of MMP-3 but not MMP-1 in an in vitro rabbit flexor tendon model

Asundi

Rempel

2008

Clinical Biomechanics

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“…However, there are few reports that discuss the underlying mechanism of these reactions in cervical intervertebral discs. Several in vitro studies [16,19,33] showed that the articular chondrocytes are regulated by mechanical load. Honda et al [16] indicated that excessive mechanical load directly alters the metabolism of cartilage by reducing the matrix components and causing a quantitative imbalance between MMPs and tissue inhibitor of metalloproteinases.…”

Section: Discussionmentioning

confidence: 99%

Herniated and Spondylotic Intervertebral Discs of the Human Cervical Spine: Histological and Immunohistochemical Observations

Kokubo

Kobayashi

Uchida

et al. 2004

Acta Histochem. Cytochem

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We investigated the histological and immunohistochemical changes within and around the cervical intervertebral discs in symptomatic myeloradiculopathy and myelopathy patients. A total of 163 cervical intervertebral discs, 100 herniated discs and 63 spondylotic discs, were harvested en bloc during anterior decompressive surgeries and histological examination was performed, focusing on nucleus pulposus, annulus fibrosus, cartilaginous endplate, and ligamentous enthesis. There was a close relationship between MRI grade and histological degenerative changes. Histological features of the herniated intervertebral discs were the presence of granulation tissue, newly-developed blood vessels and infiltration of CD68-positive cells, which surrounded the herniated tissue in the outer layer of the annulus fibrosus. Marked vascular invasion was detected in 67% of herniated discs and 54% of spondylotic discs. Immunohistochemically, chondrocytes positive for matrix metalloproteinase (MMP)-3, tumor necrosis factor (TNF)-a, basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) were detected in both herniated and spondylotic discs. Our results indicate that the neoangiogenesis process is associated with overexpression of bFGF and VEGF, and suggest that intervertebral disc degradation is enhanced by macrophage infiltration, which is regulated by MMP-3 and TNF-a. The expression of these proteins may be enhanced by the tear of annulus fibrosus.

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“…By contrast, cyclic tension applied to chondrocytes increased the expression of MMP-13 (collagenase-3), a protease responsible for the removal and cleavage of type II collagen from the matrix before vascular invasion, and CTGF, an angiogenic factor found in hypertrophic chondrocytes. The upregulation of proinflammatory proteins in chondrocytes subject to high tensile strain, may result from the pathological spindle-shaped morphology the cells acquire during this kind of loading (Honda et al 2000). Interestingly, many other cell types that are usually subjected to tension in vivo respond to applied tensile loading by downregulating synthesis of MMPs and proinflammatory substances (Long et al 2001;Sun & Yokota 2001).…”

Section: Experimental Mechanobiology and Model Validationmentioning

confidence: 99%

Modelling cartilage mechanobiology

Carter

Wong

2003

Phil. Trans. R. Soc. Lond. B

149

124

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The growth, maintenance and ossification of cartilage are fundamental to skeletal development and are regulated throughout life by the mechanical cues that are imposed by physical activities. Finite element computer analyses have been used to study the role of local tissue mechanics on endochondral ossification patterns, skeletal morphology and articular cartilage thickness distributions. Using single-phase continuum material representations of cartilage, the results have indicated that local intermittent hydrostatic pressure promotes cartilage maintenance. Cyclic tensile strains (or shear), however, promote cartilage growth and ossification. Because single-phase material models cannot capture fluid exudation in articular cartilage, poroelastic (or biphasic) solid/fluid models are often implemented to study joint mechanics. In the middle and deep layers of articular cartilage where poroelastic analyses predict little fluid exudation, the cartilage phenotype is maintained by cyclic fluid pressure (consistent with the single-phase theory). In superficial articular layers the chondrocytes are exposed to tangential tensile strain in addition to the high fluid pressure. Furthermore, there is fluid exudation and matrix consolidation, leading to cell 'flattening'. As a result, the superficial layer assumes an altered, more fibrous phenotype. These computer model predictions of cartilage mechanobiology are consistent with results of in vitro cell and tissue and molecular biology experiments.

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The effects of high magnitude cyclic tensile load on cartilage matrix metabolism in cultured chondrocytes

Cited by 139 publications

References 51 publications

Cyclic loading inhibits expression of MMP-3 but not MMP-1 in an in vitro rabbit flexor tendon model

Cyclic loading inhibits expression of MMP-3 but not MMP-1 in an in vitro rabbit flexor tendon model

Herniated and Spondylotic Intervertebral Discs of the Human Cervical Spine: Histological and Immunohistochemical Observations

Modelling cartilage mechanobiology

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