The effects of gut indigenous microbiota on intensity of oxidative stress and the cytokine immunity in women with recurrent pyelonephritis.

Stepanova, N.; Driianska, V.; Korol, L.; Mihal, L. Ya; Savchenko, V.

doi:10.26641/2307-0404.2018.1(part1).127251

Cited by 4 publications

(6 citation statements)

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“…Tasian and colleagues (16) have identified a strong association between oral antibiotics and the risk of nephrolithiasis which remains elevated for up to five years from antibiotic exposure. These results also accord with our earlier studies, which reported a strong association between antibiotics administration, intestinal barrier dysfunction and hyperoxaluria formation (15,18,25). In view of all that has been mentioned so far, one may suppose that Lactobacillus and Bifidobacterium deficiency leads to intestinal barrier dysfunction, violation of oxalate transport mechanisms in intestinal epithelium (both secretion and absorption) and a decrease in total ODA in fecal microbiota in PD patients.…”

Section: Discussionsupporting

confidence: 92%

“…In the context of dysbiosis, the only possible explanation for greater total fecal ODA loss in PD patients compared with HD patients may be the frequent use of antibiotic therapy for PD-associated infections (31). Significant contribution of ODB to oxalate homeostasis (reducing oxalate absorption and subsequent urinary excretion) after antibiotic treatment has been profoundly described (15)(16)(17)(18). Georgieva et al (29) have highlighted that most strains of the Lactobacillus and Bifidobacterium group are susceptible to the main antibiotic classes.…”

Section: Discussionmentioning

confidence: 99%

“…albigula) has been hypothesized to play a role in this process (9,13,14). The deficiency of ODB in the gut due to the antibiotic treatment is considered as one of the potential causes for upregulation of intestinal oxalate absorption (15)(16)(17)(18). Most of the existing studies are based on the quantitative determination of ODB in coprofiltrates.…”

Section: Introductionmentioning

confidence: 99%

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A potential role of fecal oxalate-degrading activity in oxalate homeostasis in end-stage renal disease patients; a descriptive pilot study

et al. 2021

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Introduction: End-stage renal disease (ESRD) patients have significant differences in plasma oxalic acid (POx) concentration under the same treatment conditions. Objectives: In the present study, we adopted the method of redoximetric titration with a KMnO4 solution to evaluate the effect of total fecal oxalate-degrading activity (ODA) on oxalate homeostasis in ESRD patients which has never been reported before. Patients and Methods: A total of 56 participants were enrolled in this cross-sectional pilot study, including 24 healthy volunteers (a control reference group) and 32 ESRD patients. Among the ESRD patients, there were 21 hemodialysis (HD) and 11 peritoneal dialysis (PD) patients. Total ODA in fecal samples as well as POx concentration, daily urinary oxalate (UOx) and PD effluent oxalate excretion were determined. Cohen’s d was computed to calculate the effect size using post-hoc analysis. Results: Total ODA in fecal microbiota ranged from -23 to 24%/0.01 g of feces and was statistically higher in healthy volunteers compared with the ESRD patients. The ESRD patients with positive total fecal ODA status had higher UOx excretion level and lower POx concentration compared with the patients with negative total fecal ODA status. Cohen’s d effect size was 1.99 and 1.05, respectively. Total fecal ODA was an independent risk factor associated with POx elevation in the ESRD patients. Conclusion: Our pilot study firstly demonstrated a potential role of total fecal ODA in oxalate homeostasis in ESRD patients. The results might be useful for determining sample size considerations and providing groundwork for future research projects.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A potential role of fecal oxalate-degrading activity in oxalate homeostasis in end-stage renal disease patients; a descriptive pilot study

et al. 2021

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“…High levels of TNF-α, MCP-1 and IL-23 in the blood in adults and children confirm their important role in both AP and ChP, but MCP-1 can be considered as an indicator of acute / exacerbation of chronic pyelonephritis [10,11].…”

Section: Original Papersmentioning

confidence: 99%

Cytokines and SLPI profiles in children and adults with urinary tract infections

Kolesnуk

Driianska

Stepanova

et al. 2019

Ukr. J. Nephrol. and Dial.

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Pro- and anti-inflammatory cytokines, SLPI and NGAL are involved in anti-infectious immunity. Studies of these indicators’ role in patients with urinary tract infections (UTIs) may determine their significance as diagnostic and prognostic markers in the case of pyelonephritis and cystitis. The objective of our study was to investigate plasma and urine cytokines and SLPI levels in patients with UTIs, determine the features in children and adults. Materials and methods. ELISA method and related test systems - «Immunotech», «Diaclon» (France); «DRG» (Germany), «Hycult biotechnology» ((Netherlands) were used to study blood cytokine levels in 118 adults and 67 children, SLPI levels in the blood and urine in 59 and 58, respectively. NGAL serum levels of 26 adults with acute pyelonephritis (AP) and 30 adults with chronic pyelonephritis (ChP) were studied using «Human lipocalin-2 / NGAL ELIZA» (Biovendor, Czech Republik) for NGAL. Comparison groups included 10 healthy donors and 11 patients with acute kidney injury (AKI). Results. The study showed an increase in pro- (IL-1, -17, -18, -23, TNF-α, MCP-1) and anti-inflammatory cytokines (IL-17, TGF-β), SLPI, NGAL in patients with UTI, some features in the case of chronic cystitis (ChC), AP and ChP. In adults, TNF-α in the blood and urine, IL-17 in the blood was higher in the case of ChC than ChP. The analysis showed a significant increase in all studied indicators’ levels for AP and ChP in children and adults. The average MCP-1 level in patients with AP was significantly higher than ChP, whereas TNF-α did not differ. In adults, IL-18 and IL-23 were highest in the case of AP, and TGF-β was the highest in the case of ChP. MCP-1, IL-23 levels in the blood of adults were higher than in children in the case of AP, and TNF-α - in the case of ChP. SLPI is involved in the AP pathogenesis and ChP exacerbation. High SLPI levels have been determined in serum and urine (NGAL in the blood) in patients with pyelonephritis (NGAL - AP) who can be used, as well as cytokines, as additional diagnostic and prognostic markers. Conclusions. High levels of TNF-α, MCP-1, and IL-23 in the blood of adults and children confirm their important role in both AP and ChP, but MCP-1 can be considered as an AP predictor/ ChP exacerbation. According to the studied cytokines, adults have a more significant immune response. The SLPI level is an additional feature for diagnosing and monitoring the course of pyelonephritis and cystitis.

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“…Patients with RUTIs must constantly take antibiotics, including long-term antibiotic prophylaxis ( Fine et al, 2019 ; Issakhanian and Behzadi, 2019 ; Toor et al, 2019 ; Meštrović et al, 2020 ). Although antibiotics prevent UTI recurrences, antibiotic-induced dysbiosis leads to increased production and accumulation of LPSs and other toxic products of bacterial activity (e.g., P-cresol, trimethylamine-N-oxidoreductase, and indoxyl sulfate) and intestinal epithelial barrier dysfunction that accelerates excessive production of cytokines and chemokines and facilitates their translocation, which causes extraintestinal and/or systemic inflammation ( Driyanska et al, 2014 ; Francino, 2016 ; Stepanova et al, 2018a ; Gagliardi et al, 2018 ). It should be noted that the effect of antibiotics on intestinal barrier function is still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

How Advanced Is Our Understanding of the Role of Intestinal Barrier Dysfunction in the Pathogenesis of Recurrent Urinary Tract Infections

Stepanova

2022

Front. Pharmacol.

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A comprehensive understanding of urinary tract infections (UTIs), one of the most common human infections, is required as they are complex and poorly understood diseases. Periurethral and vaginal colonization by rectal flora, with the constant presence of pathogens in the urethra, is the initial step of the recurrent UTIs pathway. Current scientific data describe the genetic, etiological, biological, and behavioral risk factors for recurring UTIs, but they do not include the effect of intestinal barrier function on the disease. Although gut microbiota has been proposed as the main source for UTIs, the cross-talk between intestinal barrier dysfunction and the recurrence of UTIs has not yet been supported by scientific data. In this opinion review, based on published data and the results of our clinical studies, I aimed to outline the possible contribution of intestinal barrier dysfunction to the pathogenesis of recurrent UTIs. I believe that the unanswered questions raised by this review can guide further experimental and controlled studies to clarify the mechanisms underlying the role of intestinal barrier dysfunction in the pathogenesis of recurrent UTIs.

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The effects of gut indigenous microbiota on intensity of oxidative stress and the cytokine immunity in women with recurrent pyelonephritis.

Cited by 4 publications

References 2 publications

A potential role of fecal oxalate-degrading activity in oxalate homeostasis in end-stage renal disease patients; a descriptive pilot study

A potential role of fecal oxalate-degrading activity in oxalate homeostasis in end-stage renal disease patients; a descriptive pilot study

Cytokines and SLPI profiles in children and adults with urinary tract infections

How Advanced Is Our Understanding of the Role of Intestinal Barrier Dysfunction in the Pathogenesis of Recurrent Urinary Tract Infections

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