A potential role of fecal oxalate-degrading activity in oxalate homeostasis in end-stage renal disease patients; a descriptive pilot study

Stepanova, N.; Tolstanova, Ganna; Korol, L.; Akulenko, Iryna; Савченко, О А; Kolesnyk, М.

doi:10.34172/jrip.2021.19

Cited by 6 publications

(13 citation statements)

References 28 publications

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“…The redoximetric titration with KMnO 4 was adopted to evaluate the total ODA of bacteria in culture media, as described previously [9,24]. In brief, an aliquot of 10 ml test solution and 10 ml Oxalate Medium (control solution) was centrifuged at 3000 g for 15 min at room temperature.…”

Section: Determination Of Odamentioning

confidence: 99%

“…The term of total ODA in fecal microbiota could be de ned as the general ability of different strains of ODB to metabolize oxalate [9]. Several in vitro studies have been devoted to oxalate degradation abilities of Oxalobacter formigenes, Bi dobacterium and Lactobacillus strains in which the e ciency degrade oxalate from 15-98% has been reported [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Synbiotic supplementation and oxalate homeostasis in rats: focus on microbiota oxalate-degrading activity

et al. 2022

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Background The present study aimed (i) to evaluate whether ceftriaxone treatment could affect not only intestinal oxalate-degrading bacteria number but their total activity to degrade oxalate and in uence oxalate homeostasis in rats, (ii) to test the effect of commercially available probiotics and a synbiotic on total fecal oxalate-degrading activity, (iii) and to estimate the ability of synbiotic to restore fecal oxalatedegrading activity and ceftriaxone-induced disruption of oxalate homeostasis in rats.Methods Twenty-eight female Wistar rats (200-300 g) were randomly divided into 4 groups (n = 7). Group 1 was treated with vehicle sterile water (0.1 ml, i.m., 14 days); Group 2 received synbiotic (30 mg/kg, per os, 14 days); Group 3 was treated with ceftriaxone (300 mg/kg, i.m., 7 days); Group 4 was supplemented with ceftriaxone and synbiotic. Oxalate-degrading bacteria number and their total activity, urinary and plasma oxalate concentrations were measured on days 1 and 57 after the treatment withdrawal.Results Ceftriaxone treatment reduced total fecal oxalate-degrading activity independently on oxalatedegrading bacteria number and increased urinary and plasma oxalate concentrations. The synbiotic had a high oxalate-degrading activity vs probiotics and was able to restore fecal oxalate-degrading activity and signi cantly decrease urinary oxalate excretion in antibiotic-treated rats.Conclusion Total fecal oxalate-degrading activity but not oxalate-degrading bacteria number should be thoroughly examined in the future to develop predictive diagnostics methods, targeted prevention and personalized treatment in kidney stone disease. Synbiotic supplementation had a bene cial effect on the total oxalate-degrading activity of gut microbiota, which resulted in decreased UOx excretion in rats.

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Section: Determination Of Odamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synbiotic supplementation and oxalate homeostasis in rats: focus on microbiota oxalate-degrading activity

et al. 2022

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“…Furthermore, the association between dialysis-related peritonitis and oxalate balance in PD patients had never been analyzed before. We have suggested 2 possible pathways of how dialysis-related peritonitis affects oxalate balance in PD patients: 1) the alteration of the peritoneal membrane could decrease the peritoneal clearance of oxalate and, consequently, its removal level, and 2) the use of antibiotics could increase intestinal oxalate absorption and hyperoxalemia (31)(32)(33)(34). The present study focused on the effect of dialysis-related peritonitis on oxalate balance through the reduction of the peritoneal oxalate removal rate in PD patients.…”

Section: Discussionmentioning

confidence: 94%

Oxalate Balance in Peritoneal Dialysis Patients: A Potential Role of Dialysis-related Peritonitis

Stepanova

Korol

Lebid

et al. 2022

In Vivo

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Background: Little evidence is available on oxalate balance in peritoneal dialysis (PD) patients. Patients and Methods: We performed a cross-sectional observational pilot study with 62 adult PD patients to document oxalate balance and explore its association with PD-related peritonitis. Plasma oxalate concentration, levels of oxalate excretion in 24-h urine, and peritoneal dialysis effluent were evaluated. The peritoneal oxalate transport status and renal and peritoneal oxalate clearances were calculated according to the PD-related peritonitis history. Results: PD patients with a history of peritonitis had a statistically significantly lower peritoneal oxalate clearance, daily peritoneal oxalate excretion, and overall oxalate removal rate compared with the peritonitis-free PD patients. They had a 4-fold risk of plasma oxalic acid increase, and even a single episode of dialysis-related peritonitis resulted in plasma oxalate elevation. Conclusion: Peritoneal oxalate clearance plays an important role in oxalate balance in PD patients and, therefore, dialysis-related peritonitis is a significant predictor for hyperoxalemia. Further well-designed clinical trials need to be undertaken before the association between peritonitis and oxalate balance in PD patients is more clearly understood.Oxalate is an ionized form of a potentially toxic oxalic acid formed from endogenously synthesized and exogenously ingested oxalates (1, 2). In physiological conditions, the bulk of circulating oxalate (90-95%) is excreted through the kidneys, whereas the remainder (5-10%) through the terminal parts of the small intestine and colon (1-4). A decline in kidney function leads to decreased oxalate clearance and, ultimately, hyperoxalemia in end-stage kidney disease (ESKD) (1, 5-7). The accumulation of oxalate is associated with oxidative stress and systemic inflammation (1, 8, 9), high cardiovascular risk (9-11), and increased mortality rate (1, 11) in patients with kidney stones and ESKD. Nevertheless, little evidence is available on oxalate balance in ESKD patients in general and peritoneal dialysis (PD) patients in particular; almost all scientific data on this issue were published during the 1980s and 1990s (6,(12)(13)(14).Worldwide, PD is a key element of kidney replacement therapy (15-17). Nonetheless, peritonitis remains one of the major challenges associated with severe clinical complications of PD despite technological advances (17,18). Detrimental effects of dialysis-related peritonitis on the characteristics of peritoneal transport and the alterations of the peritoneal membrane have been documented (19-21). However, the potential effect of dialysis-related peritonitis on oxalate balance in PD patients has never been evaluated before. We hypothesized that the alteration of the peritoneal membrane due to dialysis-related peritonitis could decrease the peritoneal clearance of oxalate and, consequently, its removal levels. To test this hypothesis, the present study aimed to define oxalate balance and explore its association with dia...

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“…In our previous report, we showed that ODA in fecal microbiota has a direct association with the percentage of renal interstitial fibrosis in rats following glycerol-induced acute kidney injury over 70 days [ 100 ]. In addition, total fecal ODA has been found to be significantly lower in patients with ESKD compared to healthy controls [ 101 , 102 ]. Interestingly, it appears that ODA, rather than the quantity of ODB in the gut microbiota, may have an impact on oxalate homeostasis [ 21 , 102 ].…”

Section: Gut-kidney Axis In Ckd Oxalate Homeostasismentioning

confidence: 99%

“…In addition, total fecal ODA has been found to be significantly lower in patients with ESKD compared to healthy controls [ 101 , 102 ]. Interestingly, it appears that ODA, rather than the quantity of ODB in the gut microbiota, may have an impact on oxalate homeostasis [ 21 , 102 ]. This hypothesis was supported by findings on the inverse association between ODA in fecal microbiota and serum indoxyl sulfate, UOx, and POx concentrations [ 101 , 102 ].…”

Section: Gut-kidney Axis In Ckd Oxalate Homeostasismentioning

confidence: 99%

Oxalate Homeostasis in Non-Stone-Forming Chronic Kidney Disease: A Review of Key Findings and Perspectives

Stepanova

2023

Biomedicines

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Chronic kidney disease (CKD) is a significant global public health concern associated with high morbidity and mortality rates. The maintenance of oxalate homeostasis plays a critical role in preserving kidney health, particularly in the context of CKD. Although the relationship between oxalate and kidney stone formation has been extensively investigated, our understanding of oxalate homeostasis in non-stone-forming CKD remains limited. This review aims to present an updated analysis of the existing literature, focusing on the intricate mechanisms involved in oxalate homeostasis in patients with CKD. Furthermore, it explores the key factors that influence oxalate accumulation and discusses the potential role of oxalate in CKD progression and prognosis. The review also emphasizes the significance of the gut–kidney axis in CKD oxalate homeostasis and provides an overview of current therapeutic strategies, as well as potential future approaches. By consolidating important findings and perspectives, this review offers a comprehensive understanding of the present knowledge in this field and identifies promising avenues for further research.

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A potential role of fecal oxalate-degrading activity in oxalate homeostasis in end-stage renal disease patients; a descriptive pilot study

Cited by 6 publications

References 28 publications

Synbiotic supplementation and oxalate homeostasis in rats: focus on microbiota oxalate-degrading activity

Synbiotic supplementation and oxalate homeostasis in rats: focus on microbiota oxalate-degrading activity

Oxalate Balance in Peritoneal Dialysis Patients: A Potential Role of Dialysis-related Peritonitis

Oxalate Homeostasis in Non-Stone-Forming Chronic Kidney Disease: A Review of Key Findings and Perspectives

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