The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'

Abstract

The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.

Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

“…In general, BA had a significantly higher effect on shoot regeneration, both in terms of shoot number and total shoot length, than m T ( Figure 1 C,D). This finding that BA increases the number of shoots more effectively than m T is consistent with the results obtained by Ördögh et al [ 59 ] for S. redliana Kárpáti, by Malá et al [ 30 ] for S. torminalis, by Ördögh et al [ 57 ] for Sorbus borbasii and by Meyer et al [ 60 ] for Hypericum L. species. On the other hand, some Sorbus species behaved differently: In S. × abscondita , shoot formation was significantly more vigorous on the multiplication medium with m T than the one with BA ( Table 1 and Table 2 ).…”

“…Similar results were obtained with S. domestica , where the addition of IBA to BA had no effect on shoot proliferation [ 37 ]. Many authors routinely used a combination of a cytokinin and the auxin IBA for proliferation without further analyses [ 30 , 41 , 43 , 57 , 59 ]. In contrast to our results, it has been reported that the combination of a cytokinin (BA) and auxin (IAA or NAA) had a synergistic effect on shoot multiplication in Sorbus commixta Hedl.…”

Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

“…The most commonly used propagation method for S. domestica L. is by seed [12], resulting in a wide genetic variability of seedlings in various aspects of the plant, fruit, and wood. On the other hand, asexual propagation methods like cuttings have not been adequately effective [13], while vegetative propagation by grafting onto quince is seldom used due to compatibility issues [14], with respect to studies on in vitro approaches [15][16][17][18][19][20][21][22]. In vitro propagation is a useful tool for both propagation and safeguarding genotypes with high genetic erosion, using methods that enhance the efficiency of materials and significantly reduce environmental impact [23][24][25].…”

Response of Rowan Berry (Sorbus redliana) Shoot Culture to Slow Growth Storage Conditions

“…S. redliana ‘Burokvölgy’ is a Hungarian cultivar, native to one valley of the Bakony Mountains. It has tolerance to drought and is a decorative ornamental tree of gardens and streets [ 7 ].…”