Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Gianguzzi, Valeria; Sottile, Francesco

doi:10.3390/horticulturae10020164

Cited by 2 publications

(1 citation statement)

References 62 publications

(68 reference statements)

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“…It can be calculated from measurements taken on the same sample at two different times without the loss of vegetal material. This index was also applied in in vitro cultures [22,24,[55][56][57]. In the current study, the RGR index demonstrated an improvement in the growth of saffron in TIS than in the semisolid medium since the first evaluation period (30 days).…”

Section: In Vitro Propagation Of Saffronsupporting

confidence: 53%

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Tarraf,

İzgü,

Şimşek

et al. 2024

Horticulturae

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The propagation of Crocus sativus L. relies exclusively on corm multiplication. As underground storage organs, corms are susceptible to a wide range of pathogens, environmental stresses, and diseases, making traditional propagation methods often ineffective with the loss of valuable material. In vitro propagation offers an alternative for the saffron culture under controlled conditions. In particular, the innovative application of the Temporary Immersion System (TIS) represents a technological advancement for enhancing biomass production with a reduction in operational costs. The current study utilized the Plantform™ bioreactor to propagate in vitro saffron corms from the ‘Abruzzo’ region (Italy), integrating machine learning models to assess its performance. The evaluation of saffron explants after 30, 60, and 90 days of culture showed a marked improvement in growth and microcorm production compared to conventional in vitro culture on semisolid medium, supported by the machine learning analysis. Indeed, the Random Forest algorithm revealed a predictive accuracy with an R2 value of 0.81 for microcorm number, showcasing the capability of machine learning models to forecast propagation outcomes effectively. These results confirm that applying TIS in saffron culture could lead to economically viable, large biomass production within a controlled environment, irrespective of seasonality. This study represents the first endeavor to use TIS technology to enhance the in vitro propagation of saffron in conjunction with machine learning, suggesting an innovative approach for cultivating high-value crops like saffron.

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Section: In Vitro Propagation Of Saffronsupporting

confidence: 53%

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Tarraf,

İzgü,

Şimşek

et al. 2024

Horticulturae

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A Temporary Immersion System as a Tool for Lowering Planting Material Production Costs Using the Example of Pennisetum × advena ‘Rubrum’

Pożoga,

Olewnicki,

Latocha

2024

Agriculture

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The aim of the study was to compare the variable costs of planting material production using the example of vitro cultures of Pennisetum × advena ‘Rubrum’. In the study, temporary immersion system (TIS)- and agar-based methods were used in innovative workday organisation. The workday structure involved a six-hour passaging period followed by a two-hour break for medium preparation, autoclaving, and maintenance tasks. The TIS was found to be more cost-effective than the agar cultures, with lower labour costs and comparable growing expenses. The most expensive element of agar production was labour which was 43% of the costs. The second biggest cost was materials and reagents which represented 25%. In a TIS, production materials and reagents are the most expensive part of production (44%), while labour represents 24% of costs. A TIS offers a much faster multiplication of plants than agar cultures. Plants obtained in the multiplication phase are two times cheaper using a TIS. Rooting accounted for a significant portion of production costs in both methods. Overall, the TIS demonstrated superior efficiency and cost-effectiveness compared to agar cultures in producing Pennisetum × advena ‘Rubrum’ plants.

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Temporary Immersion System as an Innovative Approach for In Vitro Propagation of Sorbus domestica L.

Cited by 2 publications

References 62 publications

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

A Temporary Immersion System as a Tool for Lowering Planting Material Production Costs Using the Example of Pennisetum × advena ‘Rubrum’

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