The effects of growth factors on DNA synthesis, proteoglycan synthesis and alkaline phosphatase activity in bovine dental pulp cells

Nakashima, Mami

doi:10.1016/0003-9969(92)90093-n

Cited by 85 publications

(66 citation statements)

References 50 publications

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“…Although experimental studies have shown that PDGF can stimulate cell proliferation and matrix synthesis in various connective tissues, others have shown that PDGF may inhibit or be unable to stimulate synthesis of collagen and proteoglycan in vitro (Nakashima 1992, Lepistö et al 1992. We observed only minor stimulatory effects of PDGF on collagen synthesis in proximal intrasynovial exor tendon segments, suggesting that PDGF is mainly a potent mitogen, but a modulator of matrix synthesis in different rabbit tendon segments in vitro.…”

Section: Discussionmentioning

confidence: 47%

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Dose-related cellular effects of platelet-derived growth factor-BB differ in various types of rabbit tendons in vitro

Yoshikawa¹,

Abrahamsson²

2001

Acta Orthopaedica Scandinavica

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Section: Discussionmentioning

confidence: 47%

“…PDGF-BB has been shown to be the strongest stimulator of cell proliferation in various tissues and cell types in vitro (Lepistö et al 1992, Wroblewski andEdwall 1992). However, its stimulatory effect on synthesis of matrix components has been considered less marked (Nakashima 1992, Bartold 1993.…”

mentioning

confidence: 99%

Dose-related cellular effects of platelet-derived growth factor-BB differ in various types of rabbit tendons in vitro

Yoshikawa¹,

Abrahamsson²

2001

Acta Orthopaedica Scandinavica

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“…Investigators have carried out experimental attempts in vitro to induce differentiation of pulp cells by culturing mouse dental papillae in agar microinjected with active molecules (Begue-Kirn, 1992, or more easily by obtaining long-term cultured cells from adult pulps (Tziafas et al, 1988;Bouvier et al, 1990;Veron et al, 1990;Nakashima, 1991Nakashima, , 1992Seux et al, 1991;Liang et al, 1992;Alliot-Licht et al, 1994;Nakashima et al, 1994;Shirakawa et al, 1994) grown on various substrates (collagen, fibronectin, glycosaminoglycans, calcium hydroxide etc.) or in the presence of growth factors (BMPs, TGFfs, EGF).…”

Section: Introductionmentioning

confidence: 99%

An in vitro Model of Human Dental Pulp Repair

Magloire¹,

Joffre²,

Bleicher³

1996

J Dent Res

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Abstract. Pulp tissue responds to dentin injury by laying down reactionary dentin secreted by existing odontoblasts or reparative dentin elaborated by odontoblast-like cells that differentiated from precursor cells in the absence of inner dental epithelium and basement membrane. Furthermore, growth factors or active dentin matrix components are fundamental signals involved in odontoblast differentiation. In vitro, dental pulp cells cultured under various conditions are able to express typical markers of differentiation, but no culture system can re-create pulp response to dentin drilling. This paper reports the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation (BrdU-positive cells), neovascularization (positive with antitype-IV collagen antibodies), and the presence of functional (3H proline-positive) cuboidal cells close to the injured area.After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. This tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.

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“…The activity of alkaline phosphatase (ALP) reflects anabolic hard tissue formation like regeneration and repair processes both in dentin and bone metabolism [6, 7, 8, 9, 10]. The concentration of free deoxypyridinoline (DPD) indicates degeneration processes of collagen-I in the bone [6, 11, 12].…”

Section: Introductionmentioning

confidence: 99%

IGFs, IGFBPs, IGF-Binding Sites and Biochemical Markers of Bone Metabolism during Differentiation in Human Pulp Fibroblasts

Reichenmiller¹,

Mattern²,

Ranke

et al. 2004

Horm Res Paediatr

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Objective: To investigate the role of the insulin-like growth factors (IGF) system during the differentiation of human pulp-derived fibroblasts (HPF). Methods: Primary HPF were cultured for 24 days in DMEM medium with IGF-I or IGF-II (50 ng/ml each). Cell growth and morphology, alkaline phosphatase (ALP) activity, the concentration of free deoxypyridinoline (DPD), IGF-I, -II, IGFBP-2 and -3 were studied. The number of ¹²⁵I-IGF-I binding sites was estimated by Scatchard analysis. Results: Light-microscopically visible nodules emerged during differentiation. Simultaneously, the ALP activity increased steadily between days 8 and 24, while the DPD concentration decreased by about 50%. The HPF produced high concentrations of IGF-II (2.00–1.30 µg/10⁶ cells) but low IGF-I, IGFBP-2. IGFBP-2 was not changed, IGFBP-3 increased by 65% during differentiation. The number of IGF binding sites increased from 8,500 ± 55 per cell (day 8) up to 22,000 ± 570 (day 24). Conclusion: The increasing number of IGF-binding sites accompanied by alterations in the biochemical bone markers during the differentiation of HPF suggests an autocrine/paracrine role for the IGFs in the formation of dentinal hard tissue.

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The effects of growth factors on DNA synthesis, proteoglycan synthesis and alkaline phosphatase activity in bovine dental pulp cells

Cited by 85 publications

References 50 publications

Dose-related cellular effects of platelet-derived growth factor-BB differ in various types of rabbit tendons in vitro

Dose-related cellular effects of platelet-derived growth factor-BB differ in various types of rabbit tendons in vitro

An in vitro Model of Human Dental Pulp Repair

IGFs, IGFBPs, IGF-Binding Sites and Biochemical Markers of Bone Metabolism during Differentiation in Human Pulp Fibroblasts

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