The effects of fractionated megavoltage X‐irradiation on the rat submandibular gland: an assessment by electron microscopy

Savage, N. W.; Kruger, B. J.; Adkins, K. F.

doi:10.1111/j.1834-7819.1985.tb04132.x

Cited by 21 publications

(15 citation statements)

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“…In contrast to our findings, they found full recov ery of the gland within two weeks, with a return to preirradiation morphology. In accordance to our findings, there was no coagulative ne crosis or inflammatory response [20].…”

Section: Discussionsupporting

confidence: 92%

“…These findings support the results from Reade and Steidler [18] and Espinal et al [19], who found degranulation of the se rous cells within 2 h after irradiation and the process was completed after 2 days. Studies by Savage et al [20][21][22] describe a shrinkage of acinar mucous cells after 3 days. This result was not confirmed in our material, where there was only slight vacuolization in the mucous cells.…”

Section: Discussionmentioning

confidence: 99%

“…Savage et al [20] showed ultrastructural damage 3 days after cumulative doses of 5,7.5 and 10 Gy, when some areas of the gland had severe changes ranging from atrophy to cell destruction. The severity of changes was re lated to the total cumulative dose.…”

Section: Discussionmentioning

confidence: 99%

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Alpha-Adrenergic Stimulation and Radiosensitivity on the Rat Submaxillary Gland

Norberg

Forsberg²

1987

ORL

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The submaxillary glands were exposed to fractionated irradiation comparable to 50 Gy in clinical practice and a single fraction of 50 Gy. Half the number of rats were given cyclocytidine, an alpha-adrenergic agonist which depletes the serous cell granules before irradiation was embarked upon. Our results show that irradiation causes a significant, irreversible destruction of serous cells. With cyclocytidine pretreatment, the radiodamage was diminished and this drug is suggested to act as a radioprotector, depleting secretory heavy metal granules, and thus preventing radiodamage in the submaxillary salivary glands.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Alpha-Adrenergic Stimulation and Radiosensitivity on the Rat Submaxillary Gland

Norberg

Forsberg²

1987

ORL

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“…13,14 In the present study, IPR was administered to mice to release the secretory granules from submandibular gland acinar cells before irradiating them, so that the effect of irradiation on protein synthesis and secretion could be determined histochemically. Savage et al 15 exposed the submandibular glands of rats to a 10-Gy dose of X-ray, and noted marked morphological changes after 3 days, but not after 14 days. Kondow 16 irradiated the jaw (including the parotid gland) of mice with a 10-Gy X-ray dose and observed marked morphological changes in parotid gland organelles 3 days later.…”

Section: Discussionmentioning

confidence: 99%

Radioprotection of the murine submandibular gland by isoproterenol: autoradiography study with 3H-leucine

et al. 2004

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Irradiation of the salivary glands results in the generation of free radicals from metal ions present in the secretory granules of acinar cells, a process that is believed to exacerbate radiation damage to the salivary glands. We therefore conducted a comparative investigation of radiation damage to the acinar cells of murine submaxillary glands in which granule secretion had been induced, and used autoradiography to visualize the pathological changes. Male BALB/c mice, at 8 weeks of age, were divided into four groups: a no-isoproterenol (IPR) and no-irradiation group (group I), a no-IPR, irradiated group (group II), an IPR, no-irradiation group (group III), and an IPR, irradiated group (group IV). Intraperitoneal injections of IPR were used, and 3 h later, the submaxillary region was irradiated with X-rays at a dose of 10 Gy. Three days after the irradiation, 3H-leucine was administered, and submaxillary glands were removed at predetermined times. Thin sections were prepared, and light- and electron-microscope autoradiography was performed. The number of reduced silver particles per unit acinar cell area was determined by light-microscopic autoradiography, and the proportion of reduced silver particles in the rough endoplasmic reticulum, the Golgi apparatus, and secretion granules was determined by electron-microscopic autoradiography. The result indicated that the effects of the radiation on the secretory potential of the submaxillary glands were diminished in acinar cells with a higher secretory granule content.

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“…Morphological studies of salivary glands following irradiation have used mainly conventional light and electron microscopical techniques (6)(7)(8)(9)(10). None of the studies, as far as we know, have dealt with the influence on the cytoskeleton, i.e.…”

Section: Original Articlementioning

confidence: 99%