The Effects of Focused Extracorporeal Pyrotherapy on a Human Bladder Tumor Cell Line (647V)

Abstract

focused extracorporeal pyrotherapy is able to alter or kill tumor cells in vitro.

Antitumoral Local Effect and Metastatic Risk of Focused Extracorporeal Pyrotherapy on Dunning R-3327 Tumors

“…The physical effects of pyrotherapy have not been clearly established, but consist of a combination of cavita tion and intense hyperthermia (>80°C) induced by high energy focused ultrasound, as demonstrated by studies in healthy tissues [2] and human tumor cells in vitro [3], It was therefore important to demonstrate the local effect of this technique on the malignant tumor as well as the absence of harmful effects on metastatic behavior.…”

“…These results suggest that pyrotherapy exerts a local effect on the Dunning R-3327 tumor model without increasing its metastatic potential and allows long-term survivals. Controlled therapeutic trials in human cancers are envisaged.Introduction 2], and the existence of a direct cytotoxic effect of focused ultrasound was also demonstrated by the in vitro destruc tion of tumor cells of a human bladder carcinoma cell line (647 V) [3], We then decided to evaluate the effect of focused ultra sound on a tumor model: Dunning R-3327. This model was selected as it contains several distinct and well-char acterized cell lines, particularly in terms of their metastat ic potential.…”

High-Intensity Focused Ultrasound: Current Potential and Oncologic Applications

“…There are a limited number of such acoustic windows because bone, air, and gas interfere with the propagation of ultrasound beams into the body, thus obscuring targets beyond these interfaces. For example, the bladder is an excellent acoustic window, and treatment results of urinary bladder tumors with HIFU have been published [127][128][129][130][131].…”

Focused Extracorporeal Pyrotherapy

“…The colony formation test was also significantly altered: 5.7% (treated group) against 70% (control group; fig. 2) [10], Second, 5,000,000 of Dunning 3327 tumor cells were injected in the left posterior thigh of Copenhagen rats; Mat Ly Lu and G tumor cells were selected to evaluate the effect of pyrotherapy on two experimental tumors with a specific growth behavior. A sham group was also tested as control group.…”