The effects of fat-induced obesity on bone metabolism in rats

Li, Wei; Xu, Peng; Wang, Cuizhe; Ha, Xiaodan; Gu, Yajuan; Wang, Yan; Zhang, Jun; Xie, Jingui

doi:10.1016/j.orcp.2016.12.001

Cited by 25 publications

(16 citation statements)

References 21 publications

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“…These structural changes are associated with a decrease in bone formation and an increase in bone resorption in HFD-fed rats [38]. In accordance with the previous studies [37,39], this study suggested that the rats model of trabecular bone loss can be established by DIO. Of note, DIO failed to induce trabecular bone loss in the treatment of vaspin, indicating that vaspin played a critical role in the pathological process of obesity-related trabecular bone loss.…”

Section: Discussionsupporting

confidence: 90%

Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway

Wang

Chen

et al. 2020

Nutr Metab (Lond)

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Background: Visceral adipose tissue-derived serine protease inhibitor (vaspin), an adipose-derived hormone, exhibits various biological functions. Recently, studies showed that vaspin is closely related to bone metabolism. However, how vaspin influences bone formation and its underlying mechanisms in high fat-induced obese rats and rat primary osteoblasts (OBs) are not fully understood. In this study, the effects of vaspin on bone mechanical parameters and microarchitecture were evaluated.Methods: A total of 40 male Sprague-Dawley (SD) rats at 5-week old were fed with high fat diet (HFD) and normal diet (ND) for 12 weeks followed by treatment of vaspin for 10 weeks. Micro CT and three-point bending tests were conducted to evaluate bone microstructure and biomechanics. The alkaline phosphatase (ALP) activity, expression of Runt-related transcription factor 2 (Runx2), Osterix (Osx), Collegen alpha1 (Colla1) procollagen I N-terminal peptide (PINP), C-telopeptide of type I collagen (CTX), Smad2/3 and p-Smad2/3 was detected by different methods. Results: Our data indicated that, compared with ND rats, HFD rats exhibited high body weight, decreased bone strength and deteriorative bone quality. In contrast, vaspin reduced the body weight, improved the whole body metabolic status, enhanced bone strength, trabecular bone mass, and expression of Runx2, Osx, PINP, and decreased the expression level of plasma CTX. In vitro studies showed that vaspin promoted osteogenic differentiation and ALP activity in rat primary OBs in a dose dependent manner. Vaspin also upregulated mRNA expression of osteogenesis-related genes Runx2, Osx and Colla1 and protein expression of Runx2, Smad2/3 and p-Smad2/3. Conclusions:Our results indicated that vaspin protects against HFD-induced bone loss, and promotes osteogenic differentiation by activating the Smad2/3-Runx2 signaling pathway.

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Section: Discussionsupporting

confidence: 90%

Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway

Wang

Chen

et al. 2020

Nutr Metab (Lond)

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“…Adiponectin also has indirect effects on bone that may be mediated via modulations of growth factor actions or insulin sensitivity [50]. Fourth, a high fat intake may interfere with intestinal calcium absorption and therefore reduce the availability of calcium for bone formation [51].…”

Section: Discussionmentioning

confidence: 99%

Associations of Weight-Adjusted Body Fat and Fat Distribution with Bone Mineral Density in Chinese Children Aged 6–10 Years

Liang

Chen

Zhang

et al. 2020

IJERPH

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Although obesity is considered osteoprotective, the effects of body fat and fat distribution on bone tissue after adjusting for the effects of body weight remain uncertain. This study evaluated the relationships between fat mass, fat distribution, and bone mineral status beyond its weight-bearing effect. We recruited 466 children aged 6-10 years in China. Dual-energy X-ray absorptiometry was used to determine the bone mineral density (BMD) and bone mineral content (BMC) in the total body and total body less head (TBLH), as well as the fat mass (FM) and percentage fat mass (%FM) of the total and segmental body. Weight-adjusted measures of FM and %FM were derived using the residual method. After adjusting for the effects of covariates, we observed statistically significant, dose-dependent negative relationships between the TBLH·BMD/BMC and various weight-adjusted measures of body fat (p for trend: <0.001-0.038). For each standard deviation increment in the weight-adjusted total body, TBLH, trunk and limbs, the size-adjusted BMC decreased approximately 9. 44, 9.28, 8.13, and 6.65 g in boys, respectively, and by approximately 13.74, 13.71, 7.84, and 12.95 g in girls, respectively. Significant inverse associations between FM accumulation in the total body and most body parts with the BMD/BMC were observed in both boys and girls after adjusting for weight and potential confounders.

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“…APN is a fat factor specifically secreted by the adipose tissue, and its level in the circulation is closely related to insulin sensitivity. As one of the receptors of APN, the expression of AdipoR2 is the highest in the liver, and after APN binds with it, its physiological roles can be activated, thus activating the peroxisome proliferator-activated receptor (PPAR-α) and resulting in energy consumption and fatty acid oxidation 13 . P38MAPK is a member of the protein serine / threonine kinase family widely distributed in vivo, and p38MAPK activation is also involved in the fatty acid metabolism in the liver.…”

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confidence: 99%

Impact of 1,25(OH) 2 D 3 on TG content in liver of rats with type 2 diabetes

et al. 2018

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Purpose: To evaluate the effects of 1,25 dihydroxy vitamin D3 (1,25(OH) 2 D 3 ) on the content of triglyceride (TG), as well as on the gene and protein expressions of adiponectin receptor 2 (AdipoR2), p38 mitogen-activated protein kinase (P38MAPK), and lipoprotein lipase (LPL) in the liver of rats with type 2 diabetes mellitus (T2DM) so as to provide theoretical basis for exploring the mechanism by which 1,25(OH) 2 D 3 regulates TG. Methods: Wistar rats were divided into four groups (n=25), with different treatments and detected the gene and protein expressions of AdipoR2, p38MAPK, and LPL in the liver tissue by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Meanwhile, the content of TG in the liver tissue was detected by the Enzyme-linked immunosorbent assay. Results: The expression of AdipoR2, p38MAPK, LPL gene and protein in the liver of VitD intervention group was significantly higher than that in T2DM group (P <0.05), while the TG content was significantly lower than that in T2DM group (P <0.05). Conclusion: 1,25(OH) 2 D 3 can decrease the content of TG in the liver, and its mechanism may be achieved by upregulating the expressions of AdipoR2, p38MAPK, and LPL in the liver.

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The effects of fat-induced obesity on bone metabolism in rats

Cited by 25 publications

References 21 publications

Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway

Vaspin antagonizes high fat-induced bone loss in rats and promotes osteoblastic differentiation in primary rat osteoblasts through Smad-Runx2 signaling pathway

Associations of Weight-Adjusted Body Fat and Fat Distribution with Bone Mineral Density in Chinese Children Aged 6–10 Years

Impact of 1,25(OH) 2 D 3 on TG content in liver of rats with type 2 diabetes

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