The effects of environmental conditions on persistence and inactivation of Brucella suis on building material surfaces

Calfee, M. Worth; Wendling, Morgan Q. S.

doi:10.1111/j.1472-765x.2012.03237.x

Cited by 25 publications

(4 citation statements)

References 16 publications

(26 reference statements)

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“…On the other hand, in winter and spring, cattle and sheep were in the pregnancy, abortion and production will release large amounts of Brucella in the surrounding environment, causing human infection. Studies have shown that Brucella can survive for several months in low temperatures, high humidity, and less sunshine in the winter 37 , 38 . Additionally, Human brucellosis is capable of being transmitted by fomites 39 .…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal distribution of human brucellosis in Inner Mongolia, China, in 2010–2015, and influencing factors

Liang

Liu

Yang

et al. 2021

Sci Rep

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Human brucellosis is caused by Brucella species and remains a major burden in both human and domesticated animal populations, especially in Inner Mongolia, China. The aims of this study were to analyze the spatiotemporal trends in human brucellosis in Inner Mongolia during 2010 to 2015, to explore the factors affecting the incidence of brucellosis. The results showed that the annual incidence was 29.68–77.67 per 100,000, and peaked from March to June. The majority of human brucellosis was male farmers and herdsmen, aged 40–59 years. The high-risk areas were mainly Xilin Gol League and Hulunbeier City. The incidence of human brucellosis in Inner Mongolia decreased during 2010 to 2015, although the middle and eastern regions were still high-risk areas. The regions with larger number of sheep and cattle, lower GDP per capita, less number of hospital beds, higher wind speed, lower mean temperature more likely to become high-risk areas of human brucellosis.

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Section: Discussionmentioning

confidence: 99%

Spatiotemporal distribution of human brucellosis in Inner Mongolia, China, in 2010–2015, and influencing factors

Liang

Liu

Yang

et al. 2021

Sci Rep

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“…However, the survival of methicillin-resistant S. aureus after desiccation was lower at 40% RH than at 16% RH, across varying surface properties (glass, wood, plastic, cloth; Coughenour et al, 2011 ). Similarly, Worth Calfee and Wendling (2012) demonstrated that low temperature and RH enhanced the survival of Brucella suis on various building materials. These studies support the premise of this work, that higher RH might lead to increased microbial access to water vapor, promoting faster metabolic activity and inhibiting long-term survival by decreasing the total energy available to the community.…”

Section: Resultsmentioning

confidence: 90%

Microbes at Surface-Air Interfaces: The Metabolic Harnessing of Relative Humidity, Surface Hygroscopicity, and Oligotrophy for Resilience

et al. 2016

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The human environment is predominantly not aqueous, and microbes are ubiquitous at the surface-air interfaces with which we interact. Yet microbial studies at surface-air interfaces are largely survival-oriented, whilst microbial metabolism has overwhelmingly been investigated from the perspective of liquid saturation. This study explored microbial survival and metabolism under desiccation, particularly the influence of relative humidity (RH), surface hygroscopicity, and nutrient availability on the interchange between these two phenomena. The combination of a hygroscopic matrix (i.e., clay or 4,000 MW polyethylene glycol) and high RH resulted in persistent measurable microbial metabolism during desiccation. In contrast, no microbial metabolism was detected at (a) hygroscopic interfaces at low RH, and (b) less hygroscopic interfaces (i.e., sand and plastic/glass) at high or low RH. Cell survival was conversely inhibited at high RH and promoted at low RH, irrespective of surface hygroscopicity. Based on this demonstration of metabolic persistence and survival inhibition at high RH, it was proposed that biofilm metabolic rates might inversely influence whole-biofilm resilience, with ‘resilience’ defined in this study as a biofilm’s capacity to recover from desiccation. The concept of whole-biofilm resilience being promoted by oligotrophy was supported in desiccation-tolerant Arthrobacter spp. biofilms, but not in desiccation-sensitive Pseudomonas aeruginosa biofilms. The ability of microbes to interact with surfaces to harness water vapor during desiccation was demonstrated, and potentially to harness oligotrophy (the most ubiquitous natural condition facing microbes) for adaptation to desiccation.

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“…It grows on routine culture media such as blood-and chocolate-agar, and colonies exhibit an indistinctive appearance. Although the organism does not produce spores, it may persist on inanimate surfaces for weeks and even months [16].…”

Section: Brucella: a Highly Transmissible Organismmentioning

confidence: 99%

Preventing Laboratory-Acquired Brucellosis in the Era of MALDI-TOF Technology and Molecular Tests: A Narrative Review

Yagupsky

2022

Zoonotic Diseases

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Brucellosis is one of the most common etiologies of laboratory-acquired infections worldwide, and handling of living brucellae should be performed in a Class II biological safety cabinet. The low infecting dose, multiple portals of entry to the body, the wide variety of potentially contaminated specimens, and the unspecific clinical manifestations of human infections facilitate the unintentional transmission of brucellae to laboratory personnel. Work accidents such as spillage of culture media cause only a small minority of exposures, whereas >80% of events result from unfamiliarity with the phenotypic features of the genus, misidentification of isolates, and unsafe laboratory practices such as working on an open bench without protective goggles or gloves or the aerosolization of bacteria. The bacteriological diagnosis of brucellae by traditional methods is simple and straightforward but requires extensive manipulation of the isolates, and, nowadays, many laboratory technicians are not familiar with the genotypic features of the genus, resulting in inadvertent exposure and contagion. Detection of brucellar infections by culture-independent molecular methods is safe, but the identification of the organism using MALDI-TOF technology is not hazard-free, requiring an initial bacterial inactivation step to avoid transmission. Unfortunately, these novel and safer methods are costly and frequently unavailable in resource-limited endemic countries.

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The effects of environmental conditions on persistence and inactivation of Brucella suis on building material surfaces

Cited by 25 publications

References 16 publications

Spatiotemporal distribution of human brucellosis in Inner Mongolia, China, in 2010–2015, and influencing factors

Spatiotemporal distribution of human brucellosis in Inner Mongolia, China, in 2010–2015, and influencing factors

Microbes at Surface-Air Interfaces: The Metabolic Harnessing of Relative Humidity, Surface Hygroscopicity, and Oligotrophy for Resilience

Preventing Laboratory-Acquired Brucellosis in the Era of MALDI-TOF Technology and Molecular Tests: A Narrative Review

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