The effects of diet and arginine treatment on serum metabolites and selected hormones during the estrous cycle in sheep

Kaminski, Samantha L.; Redmer, Dale A.; Bass, Casie S.; Keisler, D.H.; Carlson, Lacey S.; Vonnahme, K. A.; Dorsam, Sheri T.; Grazul-Bilska, Anna T.

doi:10.1016/j.theriogenology.2014.11.017

Cited by 25 publications

(31 citation statements)

References 75 publications

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“…Initial BW and BCS were similar for all groups (56.3±1 kg and 2.8±0.05, respectively). During study duration, C maintained BW, O ewes gained (P<0.001) 15.8±1 kg, and U ewes lost (P<0.001) 11.2±1 kg, similar to our previous studies [50,54].…”

Section: Methodssupporting

confidence: 88%

“…Design for experiment 1 is described in detail by Kaminski et al [50]. At d 0, ewes (n=101) were randomly assigned into Sal (~10 ml) or Arg (L-Arg-HCl; Sigma, St. Louis, MO, USA; 155 µmol Arg/kg BW) treatment groups.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the objective of this experiment was to determine the expression of LDs (measured by the area occupied by LDs) in endometrial compartments including luminal epithelium (LE) and endometrial glands (EG) obtained from ewes at early-, mid-and/or late-luteal phases fed control (maintenance), excess or restricted diets, and treated with saline (Sal, vehicle control), Arg or FSH. previously described [49,50]. Diets were initiated 60 days prior to the onset of estrus (day 0).…”

Section: Revisedmentioning

confidence: 99%

“…Progesterone concentration in serum was determined using a solid phase chemiluminescence, competitive binding immunoassay (Immulite 1000, Siemens), as previously described [49,50]. Each sample was run in duplicate.…”

Section: Progesterone Analysismentioning

confidence: 99%

“…Although the effects of diet on ovarian functions and the endocrine system have been described by several researchers [50,55,[57][58][59][60] Furthermore, using microarray analysis for endometrial tissues, altered expression of three genes involved in lipid metabolism has been demonstrated for moderately underfed (20% restriction) compared to control non-pregnant cows [69]. For sheep, plane of nutrition affected endometrial expression of PGR and ESR1 during the estrous cycle or early pregnancy [42,70].…”

mentioning

confidence: 99%

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Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

et al. 2017

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The aim of this study was to evaluate lipid droplet (LD) expression in uteri of FSH-treated or nontreated sheep administered with arginine (Arg) or vehicle (saline, Sal) and fed a control (C), excess (overfed, O) or restricted (underfed, U) diet. In experiment 1, ewes from each diet were randomly assigned to Arg or Sal treatments administered three times daily starting on Day 0 of the first estrous cycle until blood sample and uterine tissue collection at the early- or mid-luteal phase of the second estrous cycle or the late-luteal phase of the first estrous cycle. In experiment 2, ewes were injected twice daily with FSH on Days 13 to 15 of the first estrous cycle, and blood samples and uterine tissue were collected at the early- and mid-luteal phases of the second estrous cycle. Cryopreserved in optimum cutting temperature (OCT) compound, cross-sections of uterine horn were stained with boron-dipyrromethene (BODIPY; marker of LDs) followed by 4',6-diamidino-2-phenylindole (DAPI) staining and image analysis to determine the proportion (%) of area occupied by LD in luminal epithelium (LE) and endometrial glands (EGs). Control ewes maintained, O ewes gained, and U ewes lost body weight during the experiments. Serum progesterone concentration was not affected by nutritional plane or Arg treatment and was 5.5-fold greater in FSH- than Sal-treated ewes. LDs were detected in LE and superficial EG (close to LE) but not deep EG, or other uterine compartments, and area occupied by LD was greater in LE than in EG. In experiment 1, in LE and EG, area occupied by LDs was greater in C than in O or U; greater in Arg than in Sal; and greater at the late-, less at mid-, and least at early-luteal phases. In experiment 2, in LE and EG, area occupied by LDs was greater at mid- than in early-luteal phase. Comparison of data from FSH-treated and nontreated ewes (e.g., experiment 1 vs. experiment 2) demonstrated that FSH increased the area occupied by LD in LE and EG regardless of diet. Interactions between FSH treatment, stage of the estrous cycle, and plane of nutrition demonstrated that FSH increased the area occupied by LD in LE and EG at the mid-luteal phase in O and U. Thus, LDs are differentially distributed in uterine compartments, and area occupied by LD in endometrium is affected by nutritional plane, Arg or FSH, and stage of the estrous cycle. Such changes in dynamics of LD in the endometrium during the estrous cycle indicate their specific role in uterine functions.

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Section: Methodssupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Revisedmentioning

confidence: 99%

Section: Progesterone Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

et al. 2017

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Effects of different energy diets on DNA methylation and mRNA expression in follicle stimulating hormone receptor gene promoter region of Duolang sheep during estrus

Liu

Tao

et al. 2022

Mol Biol Rep

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Effects of different fat sources (saturated and unsaturated) on reproductive performance and biological indices of ewes during flushing period

Safdar

Sadeghi

Chamani

2017

Trop Anim Health Prod

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This study was conducted to evaluate the effect of feeding different sources of fat during flushing period on the reproductive performance, lambing percent, and twin numbers of Afshari ewes. A total of 84 ewes (mean weight 48 ± 3 kg; age: 3-4 years) were divided into seven groups of 12 animals and received flushing-specific rations for 5 weeks. The control group just received a basic ration (non-flushing). Lipid sources were calcium salt of palm oil (CaP), pure palm oil (PO), calcium salt of flaxseed (CaFL), calcium salt of sunflower oil (CaSF), flaxseed oil (FLO), and sunflower oil (SFO). Estrous cycles were synchronized in all ewes using 14-day CIDRs followed by 400-IU PMSG injection at the time of CIDR removal. Fertility and lambing percent were higher in ewes fed with diets containing calcium salts of flaxseed and SFO, as compared to other treatments. Total number of lambs in flushing treatments was significantly higher than that in the control group (p < 0.01). Serum cholesterol and progesterone levels were significantly greater in omega-3 (CaFL) and omega-6 (CaSF) treatments relative to other treatments (p < 0.01). It was concluded that supplementing the flushing diet with calcium salts of fatty acids (CSFA) increased blood metabolites and hormones related to reproductive performance; and improved fertility, lambing rate and ewes of CaFL treatment have the highest number of lambs (16 lambs) between different groups. Using saturated and unsaturated fatty acids, especially in their CSFA forms during flushing period, could improve the reproduction problems induced by progesterone deficiency, lack of durability of the fetus due to hormonal instability, and abortion control factors.

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The effects of diet and arginine treatment on serum metabolites and selected hormones during the estrous cycle in sheep

Cited by 25 publications

References 75 publications

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

Lipid droplets in the ovine uterus during the estrous cycle: Effects of nutrition, arginine, and FSH

Effects of different energy diets on DNA methylation and mRNA expression in follicle stimulating hormone receptor gene promoter region of Duolang sheep during estrus

Effects of different fat sources (saturated and unsaturated) on reproductive performance and biological indices of ewes during flushing period

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