The effects of diesel exhaust particles on neural tube development in the early stage chicken embryo

Şimşek, Hakan; Çolak, Ahmėt; Kaya, Serdar; Kutlay, Murat; Çetinkal, Ahmet; Haholu, Aptullah; Demircan, Mehmet

doi:10.5137/1019-5149.jtn.4965-11.1

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…Many studies have been done on chicken embryos to investigate the etiology of NTD. They evaluated the effects of environmental factors and drugs (Cetinkal et al, 2010;Dalgic et al, 2009;Guvenc et al, 2013;Simsek et al, 2012;Temiz et al, 2009;Whitsel et al, 2002). In this study, it was observed that the width of the neural tube was altered with the Riparin III concentrations evaluated.…”

Section: Discussionmentioning

confidence: 70%

Riparin III effect on the development of neural tube embryos of Gallus gallus

Lima¹,

Filho²,

Gutierrez³

et al. 2017

Afr. J. Pharm. Pharmacol.

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The aim of this study is to demonstrate the teratogenicity of Riparin III in embryos of Gallus gallus (domestic) at an early stage of development. This study is unprecedented in the literature. Chicken eggs were used to investigate the morphometry. All the groups were incubated at 37.2 ± 0.1°C and 60 ± 5% relative humidity for 24 h. The embryonic disc was identified and the eggs of the control group were administered 0.1 ml of saline tamponade (PBS, pH 7.0) and those of the other group were administered Riparin III in 25, 50 and 100 μg/ml doses. The eggs were closed with sterile adhesive strips and incubation was continued for another 24 h. All the eggs were then reopened and the embryos dissected from embryonic membranes and evaluated histopathologically with haematoxylin eosin dye for 15 s and washed with distilled water. An embryological development within twelve stages as classified by Hamburger and Hamilton (1951) was obtained. When compared with the control group, the width of the neural tube of the embryos was modified with all Riparin III concentrations. Longer somites resulted from the 50 and 100 μg/ml Riparina III concentrations. The function of the structures was preserved. This drug can potentially be used in pregnant women as an anxiolytic and for treating depression.

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Section: Discussionmentioning

confidence: 70%

Riparin III effect on the development of neural tube embryos of Gallus gallus

Lima¹,

Filho²,

Gutierrez³

et al. 2017

Afr. J. Pharm. Pharmacol.

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“…For example, vascularization decreases in the 72 h chicken embryo in responses to diesel exhaust particles (DEP), and between 30-80% of exposed embryos were poorly developed (Simsek et al, 2012).…”

Section: Organic Toxicants As Developmental Disruptorsmentioning

confidence: 99%

Cardio-respiratory development in bird embryos: new insights from a venerable animal model

2016

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-The avian embryo is a time-honored animal model for understanding vertebrate development. A key area of extensive study using bird embryos centers on developmental phenotypic plasticity of the cardio-respiratory system and how its normal development can be affected by abiotic factors such as temperature and oxygen availability. Through the investigation of the plasticity of development, we gain a better understanding of both the regulation of the developmental process and the embryo's capacity for self-repair. Additionally, experiments with abiotic and biotic stressors during development have helped delineate not just critical windows for avian cardio-respiratory development, but the general characteristics (e.g., timing and dose-dependence) of critical windows in all developing vertebrates. Avian embryos are useful in exploring fetal programming, in which early developmental experiences have implications (usually negative) later in life. The ability to experimentally manipulate the avian embryo without the interference of maternal behavior or physiology makes it particularly useful in future studies of fetal programming. The bird embryo is also a key participant in studies of transgenerational epigenetics, whether by egg provisioning or effects on the germline that are transmitted to the F1 generation (or beyond). Finally, the avian embryo is heavily exploited in toxicology, in which both toxicological testing of potential consumer products as well as the consequences of exposure to anthropogenic pollutants are routinely carried out in the avian embryo. The avian embryo thus proves useful on numerous experimental fronts as an animal model that is concurrently both of adequate complexity and sufficient simplicity for probing vertebrate cardio-respiratory development.

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“…Each group contained 12 embryos. After 30 hours of incubation, the eggs were in stage 9 according to Hamburger-Hamilton series (7,12). Egg shells were opened (under 4x magnifications) and then embryonic discs were identified.…”

Section: Incubation and Injection Of Chicken Embryosmentioning

confidence: 99%

Effects of the popular food additive sodium benzoate on neural tube development in chicken embryo

Emon¹,

Orakdogen²,

Uslu³

et al. 2014

Turkish Neurosurgery

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AIm: Many more additives have been introduced with the development of processed foods. Neural tube defects are congenital malformations of the central nervous system. More than 300 000 children are born with neural tube defects every year and surviving children remain disabled for life. Sodium benzoate is used intensively in our daily lives. We therefore aimed to evaluate the effects of sodium benzoate on neural tube defects in chicken embryos. mATERIAl and mEThods: Fertile, specific pathogen-free eggs were used. The study was conducted on five groups. After 30 hours of incubation, the eggs were opened under 4x optical magnification. The embryonic disc was identified and sodium benzoate solution was injected. Eggs were closed with sterile adhesive strips and incubation was continued till the end of the 72nd hour. All eggs were then reopened and embryos were dissected from embryonic membranes and evaluated histopathologically. REsulTs:We found that the development of all embryos was consistent with the stage. We detected neural tube obstruction in one embryo. Neural tube defects were not detected in any embryos. CoNClusIoN:This study showed that sodium benzoate as one of the widely used food preservatives has no effect to neural tube defect development in chicken embryos even at high doses.

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The effects of diesel exhaust particles on neural tube development in the early stage chicken embryo

Cited by 4 publications

References 23 publications

Riparin III effect on the development of neural tube embryos of Gallus gallus

Riparin III effect on the development of neural tube embryos of Gallus gallus

Cardio-respiratory development in bird embryos: new insights from a venerable animal model

Effects of the popular food additive sodium benzoate on neural tube development in chicken embryo

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