The Effects of Cryopreservation on the Cell Ultrastructure in Aquatic Organisms

Narida, Arah; Tsai, Shu-Yao; Huang, Chih‐Yang; Wen, Zhi‐Hong; Lin, Chiahsin

doi:10.1089/bio.2021.0132

Cited by 4 publications

(2 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cryopreservation of aquatic organisms is relatively recent and its combination with ultrastructural observations are even more recent 34 . Successful cryopreservation is dependent on several factors such as membrane permeability 35 , 36 , CPA toxicity and capacity of cryoprotection 37 , addition and removal of CPAs 36 , cryoinjury 38 intracellular ice formation (IIF), chilling injury or cooling and warming rates 12 .…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation

Campos,

Troncoso,

Paredes

2024

Sci Rep

View full text Add to dashboard Cite

This study examinates the challenges of cryopreserving sea urchin (Paracentrotus lividus) eggs, a task hindered by factors like low membrane permeability and high sensitivity to cryoprotective agents (CPAs). While successful cryopreservation has been achieved for some marine invertebrates, eggs remain problematic due to their unique characteristics. The study explores the impact of various CPAs and cryopreservation techniques on sea urchin eggs, employing scanning and transmission electron microscopy to analyze cellular damage. The findings reveal that exposure to low CPA concentrations (0.5 M) did not induce significant damage to eggs. However, high concentrations (3 M) proved highly detrimental. Every cryopreservation approach investigated in this study resulted in irreversible damage to the sea urchin eggs, rendering them nonviable for future use. The research sheds light on the importance of understanding the structural alterations induced by CPAs and cryopreservation methods. This knowledge is essential for refining cryopreservation methods, potentially paving the way for successful preservation of these challenging cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation

Campos,

Troncoso,

Paredes

2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Cryopreservation techniques have evolved beyond the slow, fast, and ultrafast freezing techniques that use temperature control equipment (Narida et al, 2022b). Vitrification is gaining popularity in cryopreservation because it prevents ice crystal formation when the speed of temperature conduction is increased, which significantly increases cooling rates (Liebermann et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

First successful production of adult corals derived from cryopreserved larvae

Narida

Tsai

Hsieh³

et al. 2023

Front. Mar. Sci.

Self Cite

View full text Add to dashboard Cite

Coral reefs worldwide are declining due to increasing concentrations of greenhouse gases, which, combined with local anthropogenic pressure, are exacerbating unprecedented mass coral bleaching. For corals to survive, restoring coral reefs through cryopreservation is crucial. The aim of this study was to vitrify and laser-warm Stylophora pistillata planulae to allow for feasible settlement, post-settlement survival, and the production of adult corals. The no-observed-effect concentrations were used to determine the best cryoprotective agents for S. pistillata. The larvae were then subjected to cooling and nanolaser warming (300 V, 10 ms pulse width, 2 mm beam diameter) by using two vitrification solutions (VSs; VS1: 2 M dimethyl sulfoxide and 1 M ethylene glycol [EG]; VS2: 2 M propylene glycol and 1 M EG) and gold nanoparticles. The results revealed that VS1-treated larvae had a higher vitrification rate (65%), swimming rate (23.1%), settlement rate (11.54%), and post settlement survival rate (11.54%) than those treated with VS2. Seasonal variations also affected the cryopreservation of the planulae; VS1 was more favorable for the planulae in spring than in fall. Although laser-warmed larvae developed slower morphologically than their controlled counterparts, the production of cryopreserved adult S. pistillata corals was achieved. The proposed technique can improve the cryopreservation of corals and advance efforts to protect endangered coral species.

show abstract

Wild coral oocytes are more amenable to low temperature preservation than cultured counterparts

Lin

Kuo

et al. 2023

Marine Environmental Research

View full text Add to dashboard Cite

The Effects of Cryopreservation on the Cell Ultrastructure in Aquatic Organisms

Cited by 4 publications

References 82 publications

Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation

Ultrastructural examination of cryodamage in Paracentrotus lividus eggs during cryopreservation

First successful production of adult corals derived from cryopreserved larvae

Wild coral oocytes are more amenable to low temperature preservation than cultured counterparts

Contact Info

Product

Resources

About