The Effects of Combined Treatment with Naringin and Treadmill Exercise on Osteoporosis in Ovariectomized Rats

Sun, Xiaolei; Li, Fengbo; Ma, Xinlong; Ma, Jianxiong; Zhao, Bin; Zhang, Yang; Li, Yanjun; Liu, Jianwei; Meng, Xinmin

doi:10.1038/srep13009

Cited by 39 publications

(39 citation statements)

References 38 publications

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“…The combination of drugs and mechanical stimulation could exert the important regulation of bone remodeling than drugs or mechanical stimulation alone. The combined treatment with naringin and treadmill exercise have an additive effect on reducing the risk of fracture for osteoporosis associated with estrogen deficiency, and low‐magnitude whole‐body vibration combined with alendronate also improved the trabecular architecture in ovariectomized rats . Our previous study has shown that mechanical strain‐induced osteoblast proliferation, differentiation, and mineralization by activating NF‐κB pathway in MC3T3‐E1 cells, moreover, the combination of icariin and mechanical strain stimulation improved osteoinductive potential by up‐regulating the mRNA and protein expressions of ALP, OCN and Col I in osteoblast‐like cells in vitro …”

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confidence: 97%

The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice

Wang

et al. 2017

Journal Orthopaedic Research

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Osteoporosis is a disease characterized by low bone mass and progressive destruction of bone microstructure, resulting in increasing the risk of fracture. Icariin (ICA) as a phytoestrogen shows osteogenic effects, and the mechanical stimulation has been demonstrated the improving effect on osteoporosis. The objective of this study was to investigate the effect of ICA in combination with constrained dynamic loading (CDL) stimulation on osteoporosis in ovariectomized (OVX) mice. The serum hormone levels, bone turnover markers, trabecular architecture, ulnar biomechanical properties, and the expression of osteoblast-related gene (alkaline phosphatase, ALP; osteocalcin, OCN; bone morphogenetic protein-2, BMP-2; Collagen I (α1), COL1; osteoprotegerin, OPG) and osteoclast-related genes (receptor activators of NF-κB ligand, RANKL; tartrate-resistant acid phosphatase, TRAP) were analyzed. The results showed that ICA + CDL treatment could increase the osteocalcin (20.85%), estradiol levels (20.61%) and decrease the TRAP activity (26.27%) significantly than CDL treatment. The combined treatment attenuated bone loss and biomechanical decrease more than single use of CDL treatment. ICA + CDL treatment significantly up-regulated the level of osteoblast-related gene expression and down-regulated the osteoclast-related genes expression; moreover, the combined treatment increased the ratio of OPG/RANKL significantly compared to ICA (72.83%) or CDL (65.63%) treatment alone. The present study demonstrates that icariin in combination with constrained dynamic loading treatment may have a therapeutic advantage over constrained dynamic loading treatment alone for the treatment of osteoporosis, which would provide new evidence for the clinical treatment of osteoporosis. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:1415-1424, 2018.

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confidence: 97%

The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice

Wang

et al. 2017

Journal Orthopaedic Research

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“…Also, in a recent study by Achiou et al (34), daily aerobic exercise plus the use of sclerostine antibody for 9 weeks increased the markers of bone formation, such as osteocalcin, and prevented destructive effects of glucocorticoids on bone mineral density, including bone mineral capacity, bone density, and the volume of bone mass connective tissue in male Wistar rats injected with glucocorticosteroids. Sun et al (35), also revealed that regular aerobic exercises combined with Naringin for 60 days led to an increase in the osteocalcin expression and a reduction in CTX-1 type I in male Wistar rats. Researchers believe that the effects of exercise training on the bone are particularly dependent on the intensity and duration of the exercise; the intensity or load on the bone is more important than the exercise time (36).…”

Section: Discussionmentioning

confidence: 93%

Three Months Aerobic Training Cannot Modulate C-Terminal Telopeptide of Type 1 Collagen in Cigarette Smokers

Eizadi

Davood

2019

Jundishapur J Chronic Dis Care

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Background: Osteoporosis, as an important public health problem, is associated with cigarette smoking. Objectives: The present study aimed to evaluate the impact of 3 months supervised aerobic training program on C-terminal telopeptide of type 1 collagen (CTX) as a marker of bone resorption in male smokers. Methods:In this semi-experimental study, thirty-two sedentary adult male smokers (Winter 2016, Saveh, Iran) aged 42 ± 7 years were randomly assigned to exercise group (3 months aerobic training, 3 times/weekly at 60% -75% of maximum heart rate, n = 16) or control group (no training, n = 16). Serum calcium, CTX and anthropometrical markers at before and after training were determined in exercise and control subjects. The statistical significance of the changes was evaluated by Student's t-test. The changes less than 0.05 were considered statistically significant. Results: A significant decrease was observed in anthropometrical markers by aerobic training in the exercise group (P < 0.05). No significant changes were observed for serum calcium (P = 0.126) and CTX (P = 0.835) after aerobic intervention compared with baseline levels. No significant changes were observed in all variables in the control group (P ≥ 0.05). Conclusions:These data suggest that aerobic training with mentioned details cannot improve bone resorption with emphasis on CTX in male smokers. To achieve a better conclusion, it is necessary to assess the indicative markers of both bone resorption and formation in response to exercise.

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“…In this study, the level of the bone resorption factor, CTX, was significantly decreased by IR training, which resulted in enhanced bone formation. Previous study reports that a high level of CTX is associated with lower BMD values in osteoporosis (Sun et al., ).…”

Section: Discussionmentioning

confidence: 93%

“…It has been reported that osteoblasts and osteoclasts perceive and respond to mechanical stimulation, and are regulated by the activation of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), sirtuins (SIRTs) and bone morphogenic proteins (BMPs) (Chen et al, 2008;Kanno, Takahashi, Tsujisawa, Ariyoshi, & Nishihara, 2007;Kim et al, 2016;Zhang et al, 2017). In addition, several studies have also shown that mechanical stimulation may inhibit the differentiation of osteoclasts and the activation of bone resorption (Sun et al, 2015;Suzuki, Yoshimura, Deyama, Suzuki, & Kitagawa, 2008). The effect of exercise training is related to the mechanical stimulation on bone caused by contraction of muscle (Oh, Tanaka, Naka, & Igawa, 2016).…”

Section: Introductionmentioning

confidence: 99%

Interval running training improves age‐related skeletal muscle wasting and bone loss: Experiments with ovariectomized rats

Kim

Jeon

et al. 2019

Experimental Physiology

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New Findings What is the central question of this study?What is the effect and mechanism of interval running training on age‐related muscle wasting and bone loss in an ovariectomized rat model? What is the main finding and its importance?Interval running training improved muscle growth and osteogenic differentiation by enhancing the expression of bone morphogenic proteins and sirtuins in ageing‐induced ovariectomized rats. Therefore, the repetition of low and high intensities within a single exercise bout, such as interval running training, may be recommended as a practical intervention to prevent skeletal muscle wasting and bone loss in the elderly. Abstract Effective prophylactic strategies are needed for the suppression of age‐related muscle wasting and bone loss after menopause. Exercise training is attractive due to its potential for improving energy metabolism, as well as age‐related muscle wasting and bone loss. In particular, interval running (IR) training involves a repetition of low and high intensities within a single exercise bout. Therefore, this study elucidated the effect of interval training on muscle and bone health, as well as anti‐ageing, in ovariectomized (OVX) rats. The anti‐ageing effect of IR on muscle and bone was tested using western blotting and micro‐computed tomography analysis, tartrate‐resistant acid phosphatase and immunohistochemical staining. IR significantly inhibited the expression of inflammatory molecules, and improved antioxidant activity via down‐regulation of mitogen‐activated protein kinases (MAPKs) in the ageing‐induced OVX rats skeletal muscle. IR compared with continuous running (CR) improved muscle mass and growth in OVX rats by the promotion of muscle growth‐related factors including MyoD, myogenin, phospho‐mechanistic target of rapamycin (p‐mTOR), sirtuins (SIRTs), and bone morphogenic proteins (BMPs). IR also effectively recovered OVX‐induced bone loss via the down‐regulation of bone resorption and osteoclast formation in receptor activator of nuclear factor κB ligand (RANKL)‐treated bone marrowmacrophages (BMMs). In particular, IR led to high expression of SIRT1 and 6, which promoted osteogenic differentiation and bone formation via modulating the BMP signalling pathway compared with CR training. The in vivo effect of IR was confirmed by immunohistochemical staining with the improvement of bone formation molecules such as BMPs and SIRTs. These results suggested that IR training affected myogenic and osteogenic formation. So, IR training may be considered for prevention of muscle wasting and bone loss for the elderly.

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The Effects of Combined Treatment with Naringin and Treadmill Exercise on Osteoporosis in Ovariectomized Rats

Cited by 39 publications

References 38 publications

The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice

The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice

Three Months Aerobic Training Cannot Modulate C-Terminal Telopeptide of Type 1 Collagen in Cigarette Smokers

Interval running training improves age‐related skeletal muscle wasting and bone loss: Experiments with ovariectomized rats

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