The effects of cholesterol on magnetically aligned phospholipid bilayers: a solid-state NMR and EPR spectroscopy study

Bicelles as model membranes for solid‐ and solution‐state NMR studies of membrane peptides and proteins

“…It has also been extended to electron paramagnetic resonance (EPR) to study the effect of a BtuB fragment (121), cholesterol (33), and lanthanide ions (123,124) on bicelles. These model membranes have also shown a promising application in micellar electrokinetic chromatography as they mimic physiologically relevant conditions.…”

“…Cardiolipin-doped bicelles have also been prepared to better resemble mitochondrial membranes (30). Dimyristoylphosphatidic acid (DMPA) can also be incorporated into bicelles (11,31), as can cholesterol (32)(33)(34), the effects of which have been described extensively (33,34). Positively charged bicelles can also be made with the addition of 1,2-dimyristoyl-3-trimethylammonium-propane (DMTAP) (26).…”

A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine

“…Previously, we have shown that the addition of cholesterol increases the order and decreases the fluidity of DMPC/DHPC bicelle systems with both 2 H solid-state NMR spectroscopy and EPR spectroscopy (Tiburu et al, 2001(Tiburu et al, , 2004Dave et al, 2003;Lu et al, 2004). Also, the addition of cholesterol to DMPC/DHPC magnetically aligned phospholipid bilayers increases the phase transition temperature (T m ) and the corresponding bicelle alignment temperature.…”

“…Accordingly, magnetically aligned phospholipid bilayers containing PLiPC can be studied at both the parallel and perpendicular orientations. The increase in resolution of the Tm 3+ -doped bicelle samples enables S mol order parameters of the acyl chain to be easily calculated (Dave et al, 2003;Lu et al, 2004).…”

Investigating the impact of cholesterol on magnetically aligned sphingomyelin/cholesterol multilamellar vesicles using static 31P NMR

“…However, close inspection of these simulations showed that the observed NMR spectra are inadequately reproduced. Certain experimental features are characteristic of magnetically aligned phospholipids (a dampened high frequency and an increasing ⊥ resonance with increasing temperature) (Brumm et al, 1992;Helfrich, 1973;Seelig et al, 1985;Speyer et al, 1987;Qiu et al, 1993;Pincelli et al, 1998;Malcolm et al, 2005;Reinl and Bayerl, 1992;Dolainsky et al, 1993;Pott and Dufourc, 1995;Dufourc et al, 1992;Lu et al, 2004;Maret and Dransfeld, 1977). Simulations assuming magnetic induced deformation of MLVs with a prolate ellipsoid shape, where the long (semi-major) axis is aligned parallel to the magnetic field are shown in Fig.…”