A 2H solid-state NMR spectroscopic investigation of biomimetic bicelles containing cholesterol and polyunsaturated phosphatidylcholine

Reconstitution of integral membrane proteins into isotropic bicelles with improved sample stability and expanded lipid composition profile

“…This suggests that we are observing changes in dynamic rate rather than structure. These samples demonstrate the potential for reconstitution of IMPs into isotropic bicelles with a variety of lipid compositions that better mimic native membranes for use in biophysical studies [26, 27]. …”

Model studies of cholesterol and ascorbate oxidation by copper complexes: Relevance to Alzheimer’s disease β-amyloid metallochemistry

“…Cholesterol is known to affect the dynamics and thickness of the hydrocarbon region of the membrane. Increasing the level of cholesterol reduces membrane disorder and rigidifies the membrane, leading to the destablization of the metalmediated a-helical assemblies of Ab that penetrate the lipid membranes [28,71]. Releasing Ab/Cu complexes from the membrane increases the redox activity of the complex [27].…”

When detergent meets bilayer: Birth and coming of age of lipid bicelles

“…Acidic bicelles with the addition of the charged phospholipid dimyristoylphosphatidylglycerol (DMPG) have been prepared and their stability has been investigated [105,106]. Other researchers have investigated the effect of cholesterol [107–110], of the unsaturated phospholipid 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC) [100], and of polyunsaturated phosphatidylcholine [110] added to bicelles. It was shown that sphingomyelin, a common sphingo-lipid in mammalian membranes, and DHPC can form isotropic as well as aligned bicelles [111].…”