The effects of certain angioneogenesis inhibitors in experimental endometriosis in rats

Abstract

The pathogenetic theory of retrograde outflow of endometrial cells into the peritoneal cavity at endometriosis is gaining an increasing support. Adequate blood supply and angioneogenesis play an important role in successful implantation and occurrence of ectopic foci. VEGF plays an important role in this process. Determination of the pathways affecting the activity of this factor seems to be promising in terms of its effect produced on the early pathogenetic links of endometriosis. OBJECTIVES: to determine the effectiveness of dopamine agonist cabergoline and highly selective COX-2 inhibitor celecoxib in an experimental model of external genital endometriosis in rats. METHODS. 83 outbred white female rats of Rattus Norvegicus Wistar were involved in the experiment. Experimental induction of endometriosis was performed by surgery and implantation of the autologous uterine fragments. The type and volume of experimental endometriosis lesions on the peritoneum of experimental animals were evaluated macroscopically as well as their hystologic examination were performed. RESULTS. Administration of a dopamine receptor agonist as a VEGF inhibitor separately was found to produce a pronounced inhibitory effect on ectopic endometrioid formation. However, the use of a dopamine receptor agonist in combination with a highly selective COX-2 inhibitor does not lead to potentiation or summation of their effects. At the same time, the use of COX-2 inhibitor alone has shown significantly lower efficacy than using the dopamine receptor agonist as a VEGF inhibitor. CONCLUSION. At experimental endometriosis in rats, dopamine agonists and highly selective COX-2 inhibitors were found to be one of VEGF inhibitors available.

A Comprehensive Review of the Efficacy and Safety of Dopamine Agonists for Women with Endometriosis-associated Infertility from Inception to July 31, 2022

“…Out of the 10, eight studies 68 , 112 - 118 investigated the primary outcome on reduction of endometriotic lesions after DRD2 treatment. Six out of the eight studies 112 - 115 , 117 , 118 showed significant decrease in the endometriotic lesion size. Although, there was no significant decrease in the lesion size in the study by Novella-Maestre et al, 68 it showed that it significantly reduced the percentage of active lesions which was one of the findings also by Delgado-Rosas et al 112 The other study which showed no reduction in the lesion was the recent study by Karslioglu et al 116 …”

Expression and Significance of Matrix Metalloproteinase-2 and Matrix Metalloproteinas-9 in Endometriosis

“…Images were qualified as high positive, positive, low positive, and negative with the corresponding percentage of cells. As a result, the IHC Optical Density Score was calculated by using the following formula [ 25 ]:…”

Modern Views on the Role of Progesterone in the Pathogenesis of Genital Endometrios

“…У здоровому ендометрію передача сигналів прогестерону й естрогену координується в чітко регулюючій взаємодії [7]. При ендометріозі тканина ендометрію розростаєтся за межами порожнини матки та найбільш імовірно порушується передача сигналів прогестерону і естрогену, що призводить до резистентності відносно прогестерону та домінування естрогену [8]. Гормональний дисбаланс, який виникає внаслідок порушення передачі сигналів прогестерону та естрогену, призводить до посилення запалення, що таким чином може посилювати тазовий біль [9].…”