The Effects of Calorie Restriction and Exercise on Age-Related Alterations in Corpus Cavernosum

Macit, Çağlar; Üstündağ, Ünsal Veli; Dagdeviren, Ozge C.; Mercanoğlu, Güldem; Şener, Göksel

doi:10.3389/fphys.2020.00045

Cited by 8 publications

(4 citation statements)

References 43 publications

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“…Studies have shown that the aging of smooth muscle cells and endothelial cells in the penis can cause oxidative stress, which in turn causes an inflammatory reaction. Over time, the inflammation can cause cumulative damage to the penis, including disruption of vascular endothelial integrity and reduction in penile smooth muscle content ( 42 , 43 ). It is also worth noting that most studies have demonstrated that aged-related ED has many commonalities in pathology with essential hypertension.…”

Section: The Physiology Of Penile Erection and The Pathophysiology Of Edmentioning

confidence: 99%

The role of microRNAs in erectile dysfunction: From pathogenesis to therapeutic potential

et al. 2022

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Erectile dysfunction (ED) is a common male sexual dysfunction disease, and it was predicted that the number of ED patients worldwide will reach 322 million by 2025. However, the pathogenesis of ED is complex and the current treatment options are still limited, so it is urgent to explore new treatment strategies. Recent studies have shown that microRNAs (miRNAs) play an important role in ED, and these single-stranded non-coding small RNA molecules are involved in key pathophysiological processes in the occurrence and development of ED. Therefore, miRNAs have remarkable potential as therapeutic targets in ED. Here, this review introduces the physiological basis of erectile function and the pathophysiological changes in ED and summarizes the current knowledge on the expression, biological functions, and molecular mechanisms of miRNAs in ED, especially the potential of miRNA-targeted therapies to improve ED. This review will provide a comprehensive view of the role of miRNAs in the pathogenesis of ED and the potential value of miRNAs in the treatment of ED.

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Section: The Physiology Of Penile Erection and The Pathophysiology Of Edmentioning

confidence: 99%

The role of microRNAs in erectile dysfunction: From pathogenesis to therapeutic potential

et al. 2022

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“…Kaya-Sezginer and Gur [ 10 ] found that endothelial dysfunction caused by low-grade inflammation may play a pathological role in ED, thus providing a meaningful target for the treatment of ED. Macit et al [ 11 ] showed that caloric restriction and physical exercise can restore endothelial and smooth muscle cells (SMCs) in the CC by decreasing apoptosis and improving endothelial function.…”

Section: Introductionmentioning

confidence: 99%

Upregulated IGFBP3 with Aging Is Involved in Modulating Apoptosis, Oxidative Stress, and Fibrosis: A Target of Age-Related Erectile Dysfunction

Cui

et al. 2022

Oxidative Medicine and Cellular Longevity

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Aging has been deemed the primary factor in erectile dysfunction (ED). Herein, age-related changes in the erectile response and histomorphology were detected, and the relationship between aging and ED was investigated based on gene expression levels. Thirty male Sprague–Dawley (SD) rats were randomly divided into 6 groups, and intracavernous pressure (ICP) and mean arterial pressure (MAP) were measured. Subsequently, the corpus cavernosum (CC) was harvested and prepared for histological examinations of apoptosis, oxidative stress (OS), and fibrosis. Then, the microarray dataset (GSE10804) was analyzed to identify differentially expressed genes (DEGs) in ED progression, and hub genes were selected. In addition, aged CC smooth muscle cells (CCSMCs) were isolated to evaluate the function of the hub gene by siRNA interference, qRT–PCR, immunofluorescence staining, enzyme-linked immunosorbent assay, western blot analysis, CCK-8 assay, EdU staining, and flow cytometry approaches. The ICP/MAP and smooth muscle cell (SMC)/collagen ratios declined with aging, while apoptosis and OS levels increased with aging. The enriched functions and pathways of the DEGs were investigated, and 15 hub genes were identified, among which IGFBP3 was significantly upregulated. The IGFBP3 upregulation was verified in the CC of aging rats. Furthermore, aged CCSMCs were transfected with siRNA to knock down IGFBP3 expression. The viability and proliferation of the CCSMCs increased, while apoptosis, OS, and fibrosis decreased. Our findings demonstrate that the erectile response of SD rats declines in parallel with enhanced CC apoptosis, OS, and fibrosis with aging. Upregulation of IGFBP3 plays an important role; furthermore, downregulation of IGFBP3 improves the viability and proliferation of CCSMCs and alleviates apoptosis, OS, and fibrosis. Thus, IGFBP3 is a potential therapeutic target for age-related ED.

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“…6 For instance, while CF occurs rapidly following cavernous nerve injury and castration within 2-4 weeks, 7,8 in diabetes mellitus, similar morphological changes can be observed within 6-12 weeks, 9,10 and in aging signs of CF could be detected throughout the entire life of laboratory animals. 11 One of the perspective diagnostic methods for CF is microcomputed tomography (micro-CT). It provides the complex 3D visualization of fibrotic processes in various organs of laboratory animals.…”

Section: Introductionmentioning

confidence: 99%

“…Also, the time intervals of taking penile samples for these purposes vary depending on the CF model because of different dynamics of fibrogenesis caused by pathology in NO‐cGMP, TGF‐β, SHH, VEGF, and ROCK signaling pathways 6 . For instance, while CF occurs rapidly following cavernous nerve injury and castration within 2–4 weeks, 7,8 in diabetes mellitus, similar morphological changes can be observed within 6–12 weeks, 9,10 and in aging signs of CF could be detected throughout the entire life of laboratory animals 11 …”

Section: Introductionmentioning

confidence: 99%

X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

et al. 2021

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Background: Current assessment methods of penile cavernous fibrosis in animal models have limitations due to the inability to provide complex and volume analysis of fibrotic alterations. Objective:The aim was to evaluate micro-computed tomography for assessment of cavernous fibrosis and compare it with histological, histochemical, immunohistochemical, and RT-PCR analysis. Materials and methods:A controlled trial was performed involving 25 New Zealand male rabbits with induced testosterone deficiency by orchidectomy. Penile samples were obtained before and after 7, 14, 21, and 84 days from orchidectomy. We consistently performed (a) gray value analysis of corpora cavernosa 3D models reconstructed after micro-computed tomography, (b) morphometry of smooth muscles/connective tissue ratio, collagen type I/III ratio, and area of TGF-beta-1 expression in corpora cavernosa, and (c) RT-PCR of TGF-beta-1 expression.Results: Micro-computed tomography allowed visualization of penile structures at a resolution comparable to light microscopy. Gray values of corpora cavernosa decreased from 1673 (1512-1773) on the initial day to 1184 (1089-1232) on the 21st day (p < 0.005). However, on the 84th day, it increased to 1610 (1551-1768). On 21st and 84th days, there was observed a significant decrease in smooth muscle/connective tissue ratio and a significant increase in collagen type I/III ratio (p < 0.05). TGF-beta1 expression increased on the 84th day according to immunohistochemistry (p < 0.005).RT-PCR was impossible to conduct due to the absence of RNA in obtained samples after micro-CT.Discussion and conclusions: Micro-computed tomography provided 3D visualization of entire corpora cavernosa and assessment of radiodensity alterations by gray value

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The Effects of Calorie Restriction and Exercise on Age-Related Alterations in Corpus Cavernosum

Cited by 8 publications

References 43 publications

The role of microRNAs in erectile dysfunction: From pathogenesis to therapeutic potential

The role of microRNAs in erectile dysfunction: From pathogenesis to therapeutic potential

Upregulated IGFBP3 with Aging Is Involved in Modulating Apoptosis, Oxidative Stress, and Fibrosis: A Target of Age-Related Erectile Dysfunction

X‐ray micro‐computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model

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