The effects of Biarum carduchrum hydroalcoholic extract on oxidative stress and catalepsy in the 6-hydroxydopamine-induced rat model of Parkinson's disease

Abstract

Background & Objective: Parkinson is a neurodegenerative disease that leads to incurable and debilitating conditions. Herbal extracts can afford protection against neurodegenerative diseases due to their bioactive compounds. In the present study, we investigated the effect of hydro-alcoholic extract of Biarum carduchrum on catalepsy and brain oxidative stress in rat's model of Parkinson's disease. Materials & Methods: Rats were randomly divided into five groups of eight animals. The control group was left intact. Parkinsonian group received an injection of 6hydroxydopamine (6-OHDA) in the right anterior mid-brain. Extract treated groups received hydro-alcoholic extract of B. carduchrum at doses of 100, 200 and 400 mg/kg by gavage seven days after 6-OHDA injection. 14 days after treatment, bar test was performed and lipid peroxide levels of different brain regions were determined. Data were analyzed by ANOVA followed by Tukey's test using SPSS22 software and P<0.05 was considered statistically significant. Results: In 6-OHDA-lesioned group bar time was increased significantly (P<0.05) when compared with the control group (122.50±90.12 versus 0.00±0.00). B. carduchrum at doses of 200 and 400 mg/kg significantly reduced 6-OHDA induced catalepsy (P<0.0.5). 6-OHDA treatment lead to significant increases in lipid peroxide levels of cerebellum, cortex, hippocampus and striatum (P<0.05). Administration of B. carduchrumextract at different doses caused significant reduction in the lipid peroxide levels of different brain regions (P<0.05). Conclusion: B. carduchrum extract ameliorated 6-OHDA-induced catalepsy and lipid peroxide level of brain in rat's model of Parkinson's disease.