The effects of betamethasone derivatives on endotoxin-induced uveitis in guinea pigs

Tsuji, Fumio; Sawa, Kenjiro; Ikuse, Toshimi; Shirasawa, Eiichi

doi:10.1007/s000110050230

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…In rabbits and rats, PAF receptor antagonists suppressed anterior uveitis (13)(14)(15), but the effects of PAF antagonists on anterior uveitis in other species have not been examined. We have established a guinea pig EIU model, which showed a good correlation with that in humans in structure-activity relationship studies of glucocorticoids (17,19,20). The binding affinities of PAF to the PAF receptors are almost the same between man and guinea pigs in the lung (1).…”

Section: Discussionmentioning

confidence: 98%

“…The cell number of both eyes of each animal was averaged for statistical analysis of the results. Cell infiltration into the anterior chamber was observed from 4 h through 24 h after LPS injection (about 2600 cells/ mm 3 at 8 h after LPS injection) (17). Intracameral injection of saline did not cause cell infiltration (17).…”

Section: Eiu In Guinea Pigsmentioning

confidence: 97%

“…The guinea pig EIU model was established according to the method reported previously (17). LPS solution (10 l) was injected intracamerally into both eyes of each animal using a 30-gauge needle.…”

Section: Eiu In Guinea Pigsmentioning

confidence: 99%

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The role of platelet-activating factor in cell infiltration in endotoxin-induced uveitis in guinea pigs

Tsuji

Shirasawa

1998

Current Eye Research

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Section: Discussionmentioning

confidence: 98%

Section: Eiu In Guinea Pigsmentioning

confidence: 97%

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The role of platelet-activating factor in cell infiltration in endotoxin-induced uveitis in guinea pigs

Tsuji

Shirasawa

1998

Current Eye Research

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“…Attenuation of inflammation was observed in most studies. In guinea pigs, betamethasone was shown to be a potent corticosteroid [12]. Banner et al .…”

Section: Discussionmentioning

confidence: 99%

Betamethasone prevents virus‐induced airway inflammation but not airway hyperresponsiveness in guinea pigs

Leusink-Muis¹,

Broeke²,

Folkerts³

et al. 1999

Clin Experimental Allergy

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In this study the effect of betamethasone was investigated in guinea pigs that demonstrate airway inflammation and airway hyperresponsiveness after a viral respiratory tract infection with parainfluenza-3 (PI3) virus. Guinea pigs were pretreated with saline or betamethasone 8 mg/kg intraperitoneally twice a day for five consecutive days, starting on day 0 and ending on day 4. On day 1, the guinea pigs were inoculated with either control solution (medium) or PI3 virus. On day 5, airway responsiveness was measured. Furthermore, a blood sample was taken, lungs were lavaged, blood leucocytes were counted, and bronchoalveolar lavage (BAL) cells were counted and differentiated. Accordingly, the activity of the bronchoalveolar cells was measured by lucigenin-amplified chemiluminescence. In virus-infected guinea pigs the total bronchoalveolar cell number was increased by 44% compared with medium-treated guinea pigs. This was mainly due to the increase in macrophages (70%, P < 0.05) and eosinophils (344%, P < 0.001). The increase in both total and differential (macrophages and eosinophils) cell numbers in virus-infected guinea pigs was completely abolished in animals treated with betamethasone. Moreover, betamethasone prevented the decrease in number of blood leucocytes in virus-infected guinea pigs. In contrast, betamethasone did not prevent the increase in airway responsiveness to both histamine (>200%) and methacholine (>100%) after the virus infection. In conclusion, betamethasone treatment prevents virus-induced airway inflammation but not airway hyperresponsiveness in guinea pigs.

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“…Betamethasone (BM) group: Rats were intramuscularly injected BM at a dose of 1 mg/kg [ 23 ], day after day for 3 successive weeks.…”

Section: Methodsmentioning

confidence: 99%

The Antioxidant, Anti-Apoptotic, and Proliferative Potency of Argan Oil against Betamethasone-Induced Oxidative Renal Damage in Rats

Orabi

Allam

Shawky

et al. 2020

Biology

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The present study aimed to investigate the protective effect of argan oil (AO) against nephrotoxic effects following overdose and long-term administration of betamethasone (BM). The phytochemical compositions of AO were assessed using GC/MS. Forty eight male Wister albino rats were divided into six groups and treated for 3 successive weeks. The control group was orally administrated distilled water daily, the BM group received BM (1 mg/kg, IM, day after day), AO/0.5 and AO/1 groups received AO (0.5 mL/kg, 1 mL/kg, orally, daily, respectively), BM + AO/0.5 group and BM + AO/1 group. The results revealed that BM induced hematological changes, including reduction of red blood cells with leukocytosis, neutrophilia, monocytosis, lymphocytopenia, and thrombocytopenia. Moreover, BM caused a significant increase of serum urea and creatinine levels, and renal malondialdehyde and nitric oxide contents with significant decrease of reduced glutathione content. BM also caused vascular, degenerative, and inflammatory histopathological alterations in kidney, along with an increase in the Bax/Bcl-2 ratio, activation of caspase-3, and decrease of proliferating cell nuclear antigen expression. Conversely, the concomitant administration of AO (0.5, 1 mL/kg) with BM ameliorated the aforementioned hematological, biochemical, pathological, and histochemical BM adverse effects. In conclusion, AO has protective effects against BM-induced renal damage, possibly via its antioxidant, anti-apoptotic, and proliferative properties.

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The effects of betamethasone derivatives on endotoxin-induced uveitis in guinea pigs

Abstract: Structure-activity relationships of glucocorticoids in the guinea pig EIL model may differ from those in the rat EIU model.

Cited by 7 publications

References 17 publications

The role of platelet-activating factor in cell infiltration in endotoxin-induced uveitis in guinea pigs

The role of platelet-activating factor in cell infiltration in endotoxin-induced uveitis in guinea pigs

Betamethasone prevents virus‐induced airway inflammation but not airway hyperresponsiveness in guinea pigs

The Antioxidant, Anti-Apoptotic, and Proliferative Potency of Argan Oil against Betamethasone-Induced Oxidative Renal Damage in Rats

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