Betamethasone prevents virus‐induced airway inflammation but not airway hyperresponsiveness in guinea pigs

Leusink-Muis, A.; Broeke, Robert Ten; Folkerts, Gert; Clerck, Fred De; Nijkamp, Frans P.

doi:10.1046/j.1365-2222.1999.00014.x

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…The binding of histamine to the H 1 receptor induces progress of the allergic symptom, which is particularly prevented using histamine H 1 -receptor blockers. These drugs, known as H 1 -antihistamines, are clinically used as anti-allergic or anti-emetic drugs (De Vos, 1999; Drábiková et al ., 2002). Results published by several authors ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Modulation of metabolic activity of phagocytes by antihistamines

Lojek¹,

Čı́ž²,

Pekarová³

et al. 2011

Interdisciplinary Toxicology

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The purpose of the study was to investigate the effects of H1-antihistamines of the 1st generation (antazoline, bromadryl, brompheniramine, dithiaden, cyclizine, chlorcyclizine, chlorpheniramine, clemastine) and the 2nd generation (acrivastine, ketotifen, and loratadine) on the respiratory burst of phagocytes. Reactive oxygen species generation in neutrophils isolated from rat blood was measured using luminol-enhanced chemiluminescence. Changes in nitrite formation and iNOS protein expression by RAW 264.7 macrophages were analysed using Griess reaction and Western blotting. The antioxidative properties of drugs in cell-free systems were detected spectrophotometrically, luminometrically, fluorimetrically, and amperometrically. The majority of the H1-antihistamines tested (bromadryl, brompheniramine, chlorcyclizine, chlorpheniramine, clemastine, dithiaden, and ketotifen) exhibited a significant inhibitory effect on the chemiluminescence activity of phagocytes. H1-antihistamines did not show significant scavenging properties against superoxide anion and hydroxyl radical, thus this could not contribute to the inhibition of chemiluminescence. H1-antihistamines had a different ability to modulate nitric oxide production by LPS-stimulated macrophages. Bromadryl, clemastine, and dithiaden were the most effective since they inhibited iNOS expression, which was followed by a significant reduction in nitrite levels. H1-antihistamines had no scavenging activity against nitric oxide. It can be concluded that the effects observed in the H1-antihistamines tested are not mediated exclusively via H1-receptor pathway or by direct antioxidative properties. Based on our results, antihistamines not interfering with the microbicidal mechanisms of leukocytes (antazoline, acrivastine and cyclizine) could be used preferentially in infections. Other antihistamines should be used, under pathological conditions accompanied by the overproduction of reactive oxygen species.

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Section: Introductionmentioning

confidence: 99%

Modulation of metabolic activity of phagocytes by antihistamines

Lojek¹,

Čı́ž²,

Pekarová³

et al. 2011

Interdisciplinary Toxicology

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“…H1 antihistamines downregulate expression of adhesion molecules [soluble intercellular adhesion molecule (ICAM-I) and VCAM-I, nuclear factor kappa B (NFκB), migration, accumulation and activation of eosinophils and other inflammatory cells, and decrease pro-inflammatory cell activation and generation of superoxide radicals, leukotrienes and eosinophilic cationic protein (ECP) at low concentrations. These receptordependent effects have been shown in patients with allergic disorders at therapeutic levels [4][5][6][7][8][9][10][11].…”

Section: The Effects Of H1 Antihistaminesmentioning

confidence: 99%

New Oral Antihistamines in Pediatrics and Safety of Antihistamines in Children

Öneş¹,

Tamay

2005

CMCAIAA

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H1 antihistamines are first line drugs in the treatment of allergic rhinitis and chronic idiopathic urticaria and widely used in children as well as in adults. Although first-generation antihistamines are effective in relieving allergic symptoms, they are not preferred because of their sedative side effects. The earliest "second generation" antihistamines, terfenadine and astemizole, non-sedating alternatives to the first generation counterparts are not commonly used due to their potential arythmogenic effects. The newer second-generation antihistamines such as loratadine, fexofenadine, mizolastine, ebastine, cetirizine, levocetirizine and desloratadine have been shown to be efficacious and well tolerated with additional anti-inflammatory effects and lacking cardiotoxic potential activity in adults. The early treatment of atopic children study, the long term clinical trial with cetirizine of infants with atopic dermatitis demonstrated that cetirizine delayed the onset of asthma in patients sensitized to grass pollen or house dust mite; and also reduced the duration and the amount of topical steroids used in the treatment of atopic dermatitis. In the Preventia I study, which was designed to evaluate the efficacy of loratadine in reducing the number of respiratory infections in young children at risk of recurrent infections, loratadine was not found to be significantly different from placebo. Both drugs were found to have a similar safety profile to that of placebo confirming their long-term use in infants and children. Pediatric formulation of desloratadine, which has favorable effect on nasal congestion, is marketed worldwide now. The effectiveness of new antihistamines in the treatment of urticaria in pediatric age group is based on extrapolation of adult studies performed in this area. Further studies with new antihistamines are needed for their evidence-based use in children with urticaria and atopic dermatitis.

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“…H 1 -antihistamine dithiaden (4[3-dimethylamino (propylidiene)]-4,9dihydrothieno-(2,3b)benzo-[e]thiepin) is a member of the 1 st class of histamine H 1 -receptor blockers and is used clinically as an anti-allergic or anti-emetic drug (De Vos, 1999). The suppresive effects of this group of drugs on histamine secretion from mast cells and basophils are well known.…”

Section: Introductionmentioning

confidence: 99%

The effects of dithiaden on nitric oxide production by RAW 264.7 cells

Kráľová¹,

Pekarová²,

Drábiková³

et al. 2008

Interdisciplinary Toxicology

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As reported in our previous studies, dithiaden (an antagonist of histamine H1-receptor, used clinically as an anti-allergic or anti-emetic drug) in a concentration range of 5×10−5–10−4 M decreased the production of reactive oxygen species by phagocytes. In this study we investigated the influence of dithiaden on nitric oxide (NO) production by LPS-stimulated macrophages.The cell viability in the presence of 10−4–5×10−5 M dithiaden was evaluated by an ATP-test. RAW 264.7 cells (2.5×106/well) were preincubated with dithiaden for 60 mins and subsequently stimulated with 0.1 µg/ml of bacterial lipopolysaccharide. After incubating for 24 hours the NO production was determined spectrophotometrically using Griess reaction as a concentration of nitrites (the end product of NO metabolism) accumulated in the cell supernatants. The expression of inducible nitric oxide synthase (iNOS) in cell-lysates was evaluated using Western blot analysis. Scavenging properties of dithiaden against NO were evaluated amperometrically.Our data demonstrate that dithiaden in the concentration of 5×10−5 M (approved by ATP test as non toxic) caused a significant decrease in the accumulation of nitrites, and in addition, this decline was followed by a marked reduction of iNOS protein expression. Amperometrical analysis did not show any scavenging properties of dithiaden against NO.From this data it can be suggested that the inhibition effect of dithiaden on macrophage NO production is caused exclusively by the suppression of iNOS protein expression.

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Betamethasone prevents virus‐induced airway inflammation but not airway hyperresponsiveness in guinea pigs

Cited by 5 publications

References 36 publications

Modulation of metabolic activity of phagocytes by antihistamines

Modulation of metabolic activity of phagocytes by antihistamines

New Oral Antihistamines in Pediatrics and Safety of Antihistamines in Children

The effects of dithiaden on nitric oxide production by RAW 264.7 cells

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