The Effects of ATP Depletion on the Response of Erythrocytes to Shear Stress

Tadano, Ken; Hellums, J. D.; Lynch, Edward C.; Peck, Ernest J.; Alfrey, Clarence P.

doi:10.1007/978-3-642-67059-6_10

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…When 75% of the interpillar channels are blocked, maximum shear stress goes up to ≈200 Pa. At this shear stress, 10 min of exposure will ensue hemolysis. [23,25] Our results are consistent with previous studies showing RBCs damage after interactions with fine fibrin strands. [20] The shear stress around RBCs increases with the reduction of interpillar channel size (Figure 2g) and can reach as high as ≈1268 Pa (in devices with 10 μm interpillar gap when 90% interpillar channels are blocked).…”

supporting

confidence: 92%

Trapped Chromatin Fibers Damage Flowing Red Blood Cells

et al. 2018

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Neutrophils are the most abundant white blood cells in the circulation and serve antimicrobial functions. One of their antimicrobial mechanisms involves the release of neutrophil extracellular traps (NETs), long chromatin fibers decorated with antimicrobial granular proteins that contribute to the elimination of pathogens. However, the release of NETs has also been associated with disease processes. While recent research has focused on biochemical reactions catalyzed by NETs, significantly less is known about the mechanical effect of NETs in circulation. Here, microfluidic devices and biophysical models are employed to study the consequences of the interactions between NETs trapped in channels and red blood cells (RBCs) flowing in blood over the NETs. It has been found that the RBCs can be deformed and ruptured after interactions with NETs, generating RBC fragments. Significant increases in the number of RBC fragments have also been found in the circulation of patients with conditions in which NETs have been demonstrated to be present in circulation, including sepsis and kidney transplant. Further studies will probe the potential utility of RBC fragments in the diagnostic, monitoring, and treatment of diseases associated with the presence of NETs in circulation.

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confidence: 92%

Trapped Chromatin Fibers Damage Flowing Red Blood Cells

et al. 2018

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“…The effect may be purely mechanical in many cases, due to bulkiness of the intercalated molecules causing lateral membrane stiffness or, perhaps, due to interactions with spectrin at the membrane endoface. A chemical mechanism may also be involved, although cell metabolism is not likely to be affected here as is probably the case when adenosine is added (Tadano et al, 1977). With theophylline, we might propose an alteration of the spectrin network by enhanced phosphorylation: theophylline inhibits catabolism of cyclic adenosine 3',5'monophosphate (cAMP) through inhibition of a critical enzyme.…”

Section: Resultsmentioning

confidence: 99%

“…Blood preservatives have been designed to preserve essential features of cell viability but not with attention to the tendency to hemolyze in subsequent shear flow. The introduction of adenosine into some blood preservatives did, however, reduce such hemolysis (Tadano et al, 1977). It seems likely that other additives could have similar or superior results, and this possibility was the principal motivation for our extended work on the effects of blood additives on shear-induced hemolysis.…”

Section: Introductionmentioning

confidence: 99%

Changes in shear-induced hemolysis due to blood additives: synthetic biopolymers and asthma drugs

Lijana¹,

Monroe²,

Williams³

1986

Ind. Eng. Chem. Fund.

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Mechanical fragility of human red blood cells was evaluated by shearing the blood between rotating polyethylene disks and measuring the timedependent release of hemoglobin. Several blood additives were tested for their effect on this hemolysis: (a) two synthetic polynucleotides (polyadenylate and polycytidylate) as biopolymers with purine and pyrimidine moieties, respectively; (b) two low-molecular-weight purine derivatives (the drug theophylline and uric acid). It was found that polyadenylate always increased hemolysis, polycytidylate often reduced it, theophylline always reduced it, and uric acid was always ineffective. Drug localization data on theophylline showed a large uptake of the additive by cell membranes, the degree of hemolysis protection being proportional to the mass of the drug absorbed. Supplementary cell characterization by resistive pulse spectroscopy documented that the protective drugs caused cell volumes to increase, deformability to decrease, and osmotic fragility to decrease. Chemical and mechanical mechanisms for changes in mechanical fragility are proposed.

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“…While the methods for calculating the "internal viscosity" by means of viscosimetry were substantiated and recognized [14], it is still not known what contribution to this parameter is made by a change in the membrane properties and by a change in the internal content of the erythrocyte around which the membrane rotates in the shear flow [15].…”

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Fundamental laws of the deformational behavior of erythrocytes in shear flow

Firsov

Priezzhev

Климова

et al. 2006

J Eng Phys Thermophys

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The dependences of the deformability parameter of erythrocytes on the shear stress in the Couette flow upon a change in their membrane rigidity or internal-content viscosity have been investigated by the diffractometric (ectacytometric) method. The results obtained suggest that the yield stress of cells measured by this method reflects the deformability of erythrocyte membranes, and the slope of the deformation curve rectified in the semilogarithmic coordinate system depends on the viscosity of the internal content of the cell. A parameter such as the limit deformation of erythrocytes is primarily determined by the viscosity of the membrane cytoskeleton.Introduction. There are several methods for investigating the deformational behavior of erythrocytes: membrane drawing in a pipette and filtration of erythrocytes through microfilters, especially with the use of nucleopores with d = 5 µm (for human erythrocytes) [1-3]. Again, the centrifugation method is worth mentioning [4]. At present, the better-substantiated diffractometric method is successfully competing with them [5,6]. This method relies on laserbeam diffraction by an erythrocyte suspension in the shear flow. Erythrocytes extended in the flow can be approximated with a good degree of accuracy by ellipsoids. A measure of erythrocyte deformation in the flow as a function of the shear stress is the deformability index defined by the relation DI = (L − H)/(L + H). With the aid of this method, Morris and Williams [7] investigated the hemolysis of erythrocytes in the presence of a shear stress and associated its value with the degree of "membrane fatigue." Phillips [8] proposed a physical substantiation of the method and showed that an increase in the ellipsoid length in the shear flow for normal erythrocytes occurs in accordance with the dependence ∆L ⁄ L = 0.19 ln (τ ⁄ τ 0 ), τ 0 = 0.38 N/m 2 .Results are independent of the erythrocyte size [9], which was used to reveal the features of the deformability of erythrocytes of various mammals. In particular, it was shown that ellipsoid-like erythrocytes (e.g., in the llama) do not deform and are only oriented in the flow. In [10], a dramatic decrease in the erythrocyte deformability upon the formation of Heinz bodies in them was revealed. The investigation of the effect of oxidative stress on the erythrocyte deformability showed that the greatest damage is caused by hydrogen peroxide [11], which was confirmed later in [12].In the rheology of erythrocytes, the problem of determining their "internal viscosity," introduced by Dintenfass as a deformability criterion [13], is still to be solved. While the methods for calculating the "internal viscosity" by means of viscosimetry were substantiated and recognized [14], it is still not known what contribution to this parameter is made by a change in the membrane properties and by a change in the internal content of the erythrocyte around which the membrane rotates in the shear flow [15].The aim of the present work is to find out to what extent the dependence of the deforma...

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The Effects of ATP Depletion on the Response of Erythrocytes to Shear Stress

Cited by 3 publications

References 10 publications

Trapped Chromatin Fibers Damage Flowing Red Blood Cells

Trapped Chromatin Fibers Damage Flowing Red Blood Cells

Changes in shear-induced hemolysis due to blood additives: synthetic biopolymers and asthma drugs

Fundamental laws of the deformational behavior of erythrocytes in shear flow

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