The effects of antioxidants on semen traits and in vitro fertilizing ability of sperm from the flat-headed cat (Prionailurus planiceps)

Thuwanut, Paweena; Chatdarong, Kaywalee; Bergqvist, Ann-Sofi; Söderquist, L.; Thiangtum, Khongsak; Tongthainan, Daraka; Axnér, Eva

doi:10.1016/j.theriogenology.2011.01.024

Cited by 41 publications

(31 citation statements)

References 44 publications

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“…The rate of embryo development after IVF for hybrid embryos revealed in the present study was comparable with the rate of in vitro development for homologous domestic cat embryos reported herein and elsewhere (Kunkitti et al, 2016). This rate of embryo development after heterologous IVF reported herein was close to or even higher than that after insemination of domestic cat oocytes with frozen-thawed spermatozoa of the fishing cat (Thiangtum et al, 2006) or the flat-headed cat (Thuwanut et al, 2011); the two species belonging to the same genus Prionailurus as the Far-Eastern cat.…”

Section: Discussionsupporting

confidence: 88%

“…Along with the Far-Eastern wildcat, the genus Prionailurus includes such endangered species as the fishing cat (Prionailurus viverrinus) (Thiangtum et al, 2006) and the flat-headed cat (Prionailurus planiceps) (Wilting et al, 2010). At present, the sperm of these two species has been successfully frozen (Thiangtum et al, 2006;Thuwanut et al, 2011). With the only exception of Ha et al (2011), who cryopreserved spermatozoa of two leopard cat males of unknown subspecies, there were no attempts to freeze sperm of the Far-Eastern wildcat.…”

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confidence: 99%

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Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Amstislavsky

Brusentsev

Kizilova

et al. 2018

Reprod Domestic Animals

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The Far-Eastern wildcat (Prionailurus bengalensis euptilurus) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far-Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far-Eastern wildcat captive-born males. Epididymal spermatozoa from five adult randomly bred domestic cat males were used as a reference. The viability of frozen-thawed spermatozoa evaluated by double staining with SYBR Green I and PI followed by the subsequent confocal laser scanning microscopy (CLSM) was 38.2% ± 3.0% for the domestic cat and 38.0% ± 10.2% for the Far-Eastern wildcat. The motility of frozen-thawed spermatozoa was 30.8% ± 9.8% for the domestic cat and 33.7% ± 15.1% for the Far-Eastern wildcat. Sperm morphology was assessed by light microscopy. The total percentage of normal spermatozoa after freezing and thawing was 51.9 ± 5.9 for the domestic cat and 55.0% ± 6.4% for the Far-Eastern wildcat. Defects of flagella were the most frequently observed abnormalities in both species (32.2% ± 4.8% and 30.8% ± 4.4% of all reported anomalies for the domestic cat and Far-Eastern wildcat, respectively). Domestic cat epididymal and Far-Eastern ejaculatory spermatozoa fertilized in vitro-matured oocytes of the domestic cat (30.0% ± 5.5% and 35.5% ± 15.0%, respectively). Taken together, these results suggest that the freezing of Far-Eastern wildcat spermatozoa with CPF medium is a suitable method for Felidae cryopreservation.

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Section: Discussionsupporting

confidence: 88%

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confidence: 99%

Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Amstislavsky

Brusentsev

Kizilova

et al. 2018

Reprod Domestic Animals

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“…The use of selection methods may represent an important tool for improving the quality of sperm samples and removing the extender [5, 6], allowing us to carry out other artificial reproductive techniques such as sperm sex sorting or in vitro fertilization (IVF) [7, 8]. For success with such techniques, which are now being employed in brown bear, a high quality of sperm samples and the absence of egg yolk and glycerol are mandatory.…”

Section: Introductionmentioning

confidence: 99%

Improving sperm banking efficiency in endangered species through the use of a sperm selection method in brown bear (Ursus arctos) thawed sperm

et al. 2017

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BackgroundSperm selection methods such as Single Layer Centrifugation (SLC) have been demonstrated to be a useful tool to improve the quality of sperm samples and therefore to increase the efficiency of other artificial reproductive techniques in several species. This procedure could help to improve the quality of genetic resource banks, which is essential for endangered species. In contrast, these sperm selection methods are optimized and focused on farm animals, where the recovery task is not as important as in endangered species because of their higher sperm availability. The aim of this study was to evaluate two centrifugation methods (300 x g/20 min and 600 x g/10 min) and three concentrations of SLC media (Androcoll-Bear −80, 65 and 50%) to optimise the procedure in order to recover as many sperm with the highest quality as possible. Sperm morphology could be important in the hydrodynamic relationship between the cell and centrifugation medium and thus the effect of sperm head morphometry on sperm yield and its hydrodynamic relationship were studied.ResultsThe samples selected with Androcoll-Bear 65% showed a very good yield (53.1 ± 2.9) although the yield from Androcoll-Bear 80% was lower (19.3 ± 3.3). The latter showed higher values of motility than the control immediately after post-thawing selection. However, both concentrations of colloid (65 and 80%) showed higher values of viable sperm and viable sperm with intact acrosome than the control. After an incubation of 2 h at 37 °C, the samples from Androcoll-Bear 80% had higher kinematics and proportion of viable sperm with intact acrosome. In the morphometric analysis, the sperm selected by the Androcoll-Bear 80% showed a head with a bigger area which was more elongated than the sperm from other treatments.ConclusionsWe conclude that sperm selection with Androcoll-Bear at either 65% or 80% is a suitable technique that allows a sperm population with better quality than the initial sample to be obtained. We recommend the use of Androcoll-Bear 65% since the yield is better than Androcoll-Bear 80%. Our findings pave the way for further research on application of sperm selection techniques to sperm banking in the brown bear.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1124-2) contains supplementary material, which is available to authorized users.

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“…The detrimental effects of LPO on spermatozoa include irreversible motility loss, inhibition of fructolysis and respiration, structural damage to the cell membrane, and finally loss of sperm viability (Dunnet, ; Maxwell & Watson, ). Previous studies indicated that the supplementation of ram semen with exogenous additives, such as taurine (Bucak et al, ), reduced glutathione and catalase (Câmara et al, ), ascorbic acid (Thuwanut et al, ) and omega‐3 (Rateb, ) could prevent oxidative toxicity of spermatozoa and reduce LPO during low‐temperature liquid storage or after freeze‐thawing process. However, ram semen enrichment with glutathione, oxidized glutathione or cysteine did not affect amounts of MDA compared to control samples (Bucak, Ateşşahin, & Yüce, ).…”

Section: Discussionmentioning

confidence: 99%

Influence of idebenone on ram semen quality stored at 4°C

Eslami

Jahan-Roshan

Farrokhi‐Ardabili

2018

Reprod Domestic Animals

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Contents The current study was designed to investigate the effect of idebenone (Id), an antioxidant on ram semen quality. Semen samples were collected, pooled and diluted in a Tris‐based extender supplemented with 0, 1, 2, 4 and 8 µM idebenone. Computer‐assisted sperm analysis was used to evaluate spermatozoa kinematics. Sperm viability and membrane functionality were assessed respectively, by eosin‐nigrosin staining and HOS test. Biochemical assays were carried out to measure different metabolites in spermatozoa and medium at 0, 24, 48 and 72 hr. Total and forward progressive motility were greater in 1, 2 and 4 µM idebenone treated groups compared to control at 24, 48 and 72 hr time points (p < 0.05). Semen supplementation with Id significantly increased viability and functionality of spermatozoa membrane during storage (p < 0.05). Lower amounts of lipid hydroperoxides in medium and spermatozoa were observed in Id‐treated groups compared to control one at 24 and 48 hr of storage (p < 0.05). Medium and spermatozoa amounts of malondialdehyde and nitric oxide were less in Id 4 µM group compared to the control at 72 hr (p < 0.05). Total antioxidant capacity values and superoxide dismutase activity of spermatozoa and medium were greater in 2 and 4 µM idebenone treated groups in comparison with the control at 72 hr (p < 0.05). Results of the current study indicated that ram semen supplementation with Id at 4 µM level improved quality by ameliorating nitrosative and peroxidative stress, hence could be considered as an antioxidant additive during storage at 4°C.

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The effects of antioxidants on semen traits and in vitro fertilizing ability of sperm from the flat-headed cat (Prionailurus planiceps)

Cited by 41 publications

References 44 publications

Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Improving sperm banking efficiency in endangered species through the use of a sperm selection method in brown bear (Ursus arctos) thawed sperm

Influence of idebenone on ram semen quality stored at 4°C

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