“…The detrimental effects of LPO on spermatozoa include irreversible motility loss, inhibition of fructolysis and respiration, structural damage to the cell membrane, and finally loss of sperm viability (Dunnet, ; Maxwell & Watson, ). Previous studies indicated that the supplementation of ram semen with exogenous additives, such as taurine (Bucak et al, ), reduced glutathione and catalase (Câmara et al, ), ascorbic acid (Thuwanut et al, ) and omega‐3 (Rateb, ) could prevent oxidative toxicity of spermatozoa and reduce LPO during low‐temperature liquid storage or after freeze‐thawing process. However, ram semen enrichment with glutathione, oxidized glutathione or cysteine did not affect amounts of MDA compared to control samples (Bucak, Ateşşahin, & Yüce, ).…”